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20211
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Subject: Chemistry × Author: Arora, Aayushi × End date: 2021 × Start date: 2020 ×
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    Profiling of Micronutrients and Heavy metals in Ocimum species of Punjab
    (Punjab Agricultural University, Ludhiana, 2021) Arora, Aayushi; Tandon, Ritu
    Medicinal plants are the foundation stones of Ayurveda and Siddha system of medicine as they help in curing a variety of ailments. Genus, Ocimum represents a major member of medicinal plants of family Lamiaceae. Ocimum, also known as Tulsi, is indigenous to the Indian continent and is highly revered for its medicinal uses. Scientific examinations of the medicinal plants w.r.t elemental analysis especially heavy metals are lacking to ensure development of their safe formulations. Such studies are needed to ensure the standardization and quality control of the developed products and approval for use in primary health care. This need has greatly emphasized on the development of analytical methods for their elemental analysis to produce reliable results and hence to guarantee the quality. In present study, ICP-MS method was validated for the simultaneous determination of essential micronutrients (Co, Ni, Se, Cu, Mn, Zn, Al and Fe) and toxic elements (Cr, As, Cd, Pb and Hg) in Ocimum leaves. The extraction of samples was performed by a microwave assisted digestion with HNO3 to stabilize these elements. The validated method fulfilled all the acceptance criteria (Correlation coefficient (R) ≥ 0.995, Recoveries ranging between 70-130 % and RSD ≤ 20 %) for the parameters: linearity, limit of detection/quantification, accuracy and precision at three levels of concentrations. LOQ for all the elements varied from 0.01160.55 µg/kg. Three Ocimum species (Ocimum kilimandscharicum, Ocimum basilicum and Ocimum sanctum) collected from two different locations (PAU, Ludhiana, Punjab and Fragrance and Flavour Development Centre, Kannuaj, Uttar Pradesh) were analyzed for their micronutrient and heavy metal contents by using the validated method on ICP-MS. Analytical results demonstrated that O. kilimandscharicum contained the highest levels of micronutrients especially Fe and Al than other two species. However, among various heavy metals, concentration of Cd (in O. basilicum) and Hg (in all Ocimum species) were found to be higher than their permissible limits of 0.3 and 1.0 mg/kg respectively. The validated method, thus, can be effectively used in laboratory for routine determination of these micronutrients (8) and heavy metals (5) in Ocimum species with acceptable analytical performance.