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  • ThesisItemOpen Access
    Variation in root architecture of advance wheat lines under drought and irrigated conditions
    (Punjab Agricultural University, Ludhiana, 2021) Manpreet Kaur; Dhingra, Madhu
    In the present study, a set of thirty six advance wheat lines including four checks were evaluated for morpho-physiological and yield attributes under irrigated and restricted irrigation conditions in the field. Morphological and yield attributes revealed significant differences among the lines under both the moisture regimes, indicating genetic diversity in the set. Under restricted irrigation conditions, all the lines were affected in terms of plant height, days to flowering and maturity, number of tillers, spikelets per spike and yield attributes; though checks were capable of retaining the performance with minimum reduction in grain yield. Genotypes PBW-677, BWL-3504 and BWL-3498 recorded 0.38-1.99% reduction in yield and were comparable to checks PBW-175 (0.33%) and C-306 (0.53%). On the contrary, genotypes PBW-765, HD-2967, and BRW-3775 recorded 34.65, 38.36 and 39.47% reduction in grain yield under restricted irrigation condition. Genotypes PBW-677, BWL-3504, BWL-3498 and BWL-5233 also depicted lesser effect of water deficit on physiological and biochemical traits viz. chlorophyll content (0.29-2.36% reduction), NDVI (0-0.59% reduction), MSI (3.4-6.2%), RWC (1.0-5.6%), total soluble sugars (36-49% increase) and proline content (1-5%). Genotypes also showed variability in stomatal characteristics with lower stomatal frequency and higher stomatal size in checks. On the basis of percent reduction in grain yield, genotypes with minimum reduction (PBW-677, BWL3504, BWL-3498 and BWL-5233-category I) and maximum reduction (HD-2967, BRW-3775 and PBW-765-category II) along with checks were characterized for root architecture traits. Seminal root traits and root hair density varied under water stress, with higher number of roots and root hair density in checks and category I genotypes. Category I genotypes exhibited longer roots (78.60-88cm) with higher biomass (6.88-10g) at vegetative and reproductive stages. For shoot length and biomass, all the genotypes responded positively under irrigated regime but effect of drought was particularly significant for category II genotypes. Number of nodal roots was also higher in PBW-677 and BWL-3498 respectively. Thus higher number and length of seminal roots, length of nodal roots and total root length and biomass coupled with minimum reduction in grain yield in genotypes PBW-677, BWL3498 and BWL-3504 under restricted irrigation condition indicates the association of root traits with water use efficiency and their role in imparting drought tolerance.