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  • ThesisItemOpen Access
    Mitigation of salt stress in maize (Zea mays L.) by glycine betaine and glutathione
    (Punjab Agricultural University, Ludhiana, 2021) Chhatwal, Himani; Gupta, Namarta
    In the present investigation, the effect of glycine betaine and glutathione on physiological and biochemical parameters was studied at different salt stress levels in maize (Zea mays L.) in two cvs. J 1006 and J 1007. The salt stress had adverse effects on seed germination and seed vigour parameters. Seeds of maize were subjected to different treatments viz. hydration, glycine betaine (10 mM, 20 mM) and glutathione (100 ppm, 500 ppm) at salt stress of 50 mM, 75 mM and 100 mM NaCl. Different parameters were recorded from seedlings after 10 days of germination. The seed treatments increased the physiological (percent germination, seedling length, seedling biomass, speed of germination, vigour index I and II) and biochemical parameters (total soluble sugars, total soluble proteins, proline content and activities of antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase) of seedlings as compared to control. However, malondialdehyde content decreased with seed treatments at all salinity levels in both the cultivars. The results showed that glycine betaine 20 mM and glutathione 500 ppm were more effective than glycine betaine 10 mM and glutathione 100 ppm, respectively. Thus, the application of glycine betaine and glutathione could play an important role in improving the salt stress tolerance by osmoprotection and scavenging the free radicals.