Thumbnail Image

Thesis

Browse

20231
Reset filters
Subject: Botany × Author: Arshdeep Kaur × End date: 2024 × Start date: 2020 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemEmbargo
    Effect of biochar and bacterial endophytes on salinity tolerance in oats (Avena sativa L.)
    (Punjab Agricultural University, 2023) Arshdeep Kaur; Gupta, Namarta
    The present investigation was carried out to study the effect of biochar and plant growth-promoting bacterial endophytes for salt tolerance in oats (Avena sativa L.). Forty four morphologically distinct salinity-tolerant endophytic bacteria were isolated from the leaf, stem and root samples of oats on nutrient agar with 2.5% NaCl and screened for the production of ammonia, auxins, siderophore and HCN; solubilization of Ca3(PO4)2, ZnO, Zn3(PO4) and ZnCO3 and salt tolerance up to 12% NaCl. Bacterial isolates showing high salt-tolerance and plant growth-promoting traits were selected and tested for growth promotion of oats on 1% H2O agar plates. Three potential isolates were identified based on polyphasic approach as Enterobacter quasiroggenkampii O1SE4, Bacillus inaquosorum O3SE2 and Bacillus safensis O4SE1. The SEM analysis showed the colonization of the plant tissues by all the three bacterial strains. Total 25 genotypes were screened for germination parameters viz. percent germination, shoot length, root length, seedling length, fresh weight, dry weight, vigour index I and II, proline, SOD, POD and CAT under salt stress conditions and were grouped into highly salt tolerant, moderately salt tolerant, moderatly salt sensitive and highly salt sensitive. Among seed treatments, the combination of biochar (2%) and Bacillus safensis maximally improved the above parameters as compared to the other treatments of Enterobacter quasiroggenkampii, Bacillus inaquosorum and biochar (1%). The evaluation of biochar and bacterial strains was done in two salt tolerant and two salt sensitive genotypes grown in the pots. The highest plant height, SLW, NAR were observed with the bacterial treatment (Bacillus safensis) + biochar (2%). Among biochemical parameters chlorophyll, carotenoid, total soluble sugar, starch, total soluble protein and free amino acids along with activities of antioxidant enzymes SOD, POD and CAT were enhanced by the application of Bacillus safensis and biochar (2%). Na+/K+/Ca2+/Mg2+ homeostasis was maintained by biochar and bacteria application. In conclusion, it is stated that the combined use of biochar and halotolerant endophytic bacteria can be used for improving the crop growth under salt stress in oats.