Thumbnail Image

Thesis

Browse

2001 - 20093
Reset filters
Subject: Biochemistry × End date: 2009 × Start date: 2001 × Type: Thesis × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    ThesisItemOpen Access
    A Study of the Toxicity of Nitrosamines under hypercholesterolemic Conditions and Role of Dietary Fibre
    (Department of Biochemistry College of Basic Sciecnes & Humanities, PAU-Ludhiana, 2001) Mittal, Gaurav
  • Thumbnail Image
    ThesisItemOpen Access
    Effect of fibre digesting enzymes on in vitro and in sacco degradation of various feedstuffs in buffalo calves
    (Department of Biochemsitry, College of Basic Sciences and Humanities PAU, Ludhiana, 2001) Chopra, Smily; Sareen, V. K
  • Thumbnail Image
    ThesisItemRestricted
    Identification of drought tolerance related biochemical indices in a wheat recombinant population
    (Punjab Agricultural University, Ludhiana, 2009) Gulati, Ankit; Asthir, Bavita
    The present research was aimed at evaluation of biochemical indices of drought tolerance in wheat. Root and shoot tissue of six wheat genotypes viz.C306, C273, PBW550, PBW534, PBW175 and PBW343 were evaluated at 6 day post germination for growth parameters, relative water content (RWC), membrane stability related parameters, enzyme of carbohydrate metabolism, carbohydrate content and osmolytes accumulation under drought stress (simulated using 8% polyethylene glycol). Stress causes short stature in shoot and elongated roots in all genotypes. C306, C273, PBW534 and PBW175 were found to have longest roots under drought stress. C273, C306 and PBW343 maintained higher RWC under stress. Lowest membrane injury index (MII) and thiobarbituric acid reactive substances (TBARS) were found in C273 and PBW343. Lowest decrease in chlorophyll content (about 21%) was found in PBW175 and PBW343 under stress. The activity of sucrolytic enzymes (acid and neutral invertase) and sucrose synthase (cleavage) decreased whereas sucrose synthesizing enzymes (sucrose phosphate synthase and sucrose synthase synthesis) increased under stress in all the genotypes. Lowest sufficient sucrolytic activity was found in C273 and PBW343 whereas C306 and PBW343 maintained higher activity of sucrose synthesizing enzymes. Amylase activity decreased under stress in endosperm to have lower mobilization of starch. PBW175 and C273 were found to have highest sucrose content showing greater osmotic adjustment. Protein content decreased in contrast to amino acid under stress. C273 was found to have highest proline and amino acid content under stress. To establish cause and effect relationship between biochemical traits and drought tolerance a large randomly derived backcross recombinant inbred line (BCRIL) population derived from (C273/PBW343//PBW343) was studied at vegetative stage. Recombinant lines showing drought tolerance in the field showed an average lower proline content compared to sensitive lines. BCRIL population with low MII were drought sensitive. Drought tolerance however, showed a positive association with membrane stability. The other indices (TBARS, RWC and chlorophyll content) were not related to drought tolerance in field.