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Subject: Biochemistry × Author: Charandeep Singh × End date: 2019 × Start date: 2018 × Type: Thesis ×
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    ThesisItemOpen Access
    Evaluation of vacuolar ATPase and identification of salt stress responsive genes in cotton
    (Punjab Agricultural University, Ludhiana, 2019) Charandeep Singh; Sangha, Manjeet Kaur
    The present study evaluated the effect of salinity stress on various ATPase activities i.e vacuolar (V)-ATPase, F-ATPase and plasma (P)-ATPase in salt tolerant (LH 2108 (Gossypium hirsutum) and LD 949 (G.arboreum)) and salt susceptible (F1378, F2228 (G. hirsutum) and FDK124 (G.arboreum)) cotton genotypes. The seeds were grown in pots at salinity levels of 9, 15 and 18 dS/m. The leaf and root samples were collected at 15 and 30 days after germination. In response to salinity the V-ATPase activity registered maximum increase followed by F-ATPase, while P-ATPase registered decline. LH2108 (G. hirsutum) showed maximum increase in V-ATPase and F ATPase activities followed by that in LD 949 (G.arboreum). LH 2108 (G.hirsutum), registered 27.05 to 35.26μmol Pi formed h-1 mg-1 protein increase in mean leaf V-ATPase activity followed by that in LD 949 (G. arboreum) (27.30 to 34.25 μmol Pi formed h-1 mg-1 protein) from control to 18EC. Similar trend was observed for root samples and for protein content in leaf and root tissues. Genes (19) of salinity tolerance pathways viz. SOS4, HKT, HDG, GhPLATZ, SCARECROW, ABR1, GbRLK1, WRKY33, lysophospholipase, β-ketoacyl CoA synthase, ROS scavenging activity including GhSOD1, udp glucosyltransferase, GhMT3a, GhAnn1, ZAT10, WRKY39-1, osmolytic regulation P5CS, sucrose phosphate synthase, sorbitol dehydrogenase and trehalose synthase, selected by in silico analysis, showed their presence (by PCR amplification) in the selected (19) cotton genotypes. The salt tolerant G.hirsutum genotype showed better salinity tolerance which might be due to higher V and F-ATPase activities and also might be due to the presence of the selective salinity stress responsive genes.