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20121
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Subject: Biochemistry × Author: Arvind Kumar × End date: 2018 × Thesis Type: Ph.D ×
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    Response of sulfur and nitrogen nutrition on storage protein quality in developing seeds of mungbean [Vigna radiata(L.) Wilczek]
    (PAU, 2012) Arvind Kumar; Sharma, Sucheta
    The present study was conducted to evaluate the response of sulfur and nitrogen nutrition on storage protein quality in developing seeds of mungbean [Vigna radiata (L.) Wilczek] varieties PAU 911 and ML 818. Crops were sown in the experimental fields of Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana following recommended package of practices. Before sowing, different treatments of sulfur and nitrogen @40kg/ha were given to the fields i.e. T 1 (control), T2(single super phosphate), T3 (urea), T4 (single super phosphate and urea), T5 (gypsum), T6 (ammonium nitrate) and T7 (gypsum and ammonium nitrate). Maximum biomass accumulation was observed at 16 DAF and maximum dry weight at 20 DAF in both the varieties. Crude protein, total soluble protein, sulfur containing amino acids (methionine and cysteine) contents in developing seeds increased maximally with T5 as compared to control and other treatments. The free amino acids increased upto 20 DAF then declined towards maturity. The albumin and globulin content in developing seeds increased with various treatments of nitrogen and sulfur sources. Among the legumins and vicilin, legumin showed better response to sulfur and nitrogen nutrition, which is desirable as legumin are rich in sulfur containing amino acids. Nitrate reductase (NR), glutamate dehydrogenase (GDH), O-Acetyl serine thiol lyase (OSTL) and glutathione reductase (GR) activities were highest at 18 DAF and then declined towards maturity. NR and GDH activities were maximally increased with urea (T 3) whereas gypsum (T5) showed higher increase in OSTL and GR activities as compared to other treatments in both the varieties. Highest incorporation of C 14 leucine in albumin and globulin protein fraction of developing seeds at 16 DAF was due to gypsum treatment after 12 h and 24 h incubation periods in both the varieties. In mature seeds, gypsum (T5) gave maximum response towards protein quality parameters i.e. total sulfur, total soluble protein, sulfur containing amino acids and seed storage protein fractions. Electrophoretic studies revealed more distinct and intense banding pattern of globulin with T5 as compared to control and other treatments.