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20131
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Subject: Agricultural Biochemistry × Author: Sandeep Kaur × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    ANALYSIS OF ABA-DEPENDENT ANTIOXIDANT RESPONSE OF WHEAT SEEDLINGS UNDER THE INFLUENCE OF EXOGENOUS SUGARS
    (Punjab Agricultural University, Ludhiana, 2013) Sandeep Kaur
    Sugar(Glucose; G and Sucrose; S) regulation of seed germination, growth and antioxidant potential, and involvement of ABA biosynthesis in these regulations were studied in two wheat cultivars varying in ABA sensitivity, where C306 was ABA-higher sensitive and PBW343 was ABA-lesser sensitive. Two concentrations (1.5% and 3%) of both sugars were used for germination and growth experiments where sodium tungstate was used as inhibitor of ABA biosynthesis. Germination inhibition was observed under both sugars supply but ABA biosynthesis was involved only under 3% S in PBW343 and 1.5% S in C306 otherwise not involved in such inhibition. Growth of seedlings was inhibited under both concentration of G and under 3% S in both cultivars where ABA biosynthesis contributed in such inhibition under 1.5% G in both cultivars and 3% S in PBW343. Growth was stimulated under 1.5% S, it involved ABA biosynthesis in both cultivars. Increases in antioxidant enzymes were higher under G than under S in both cultivars and were higher in C306 than PBW343 under both sugar supply. These increases involved ABA biosynthesis in both cultivars. Reduced state of ascorbate was affected under G while increased under S in roots and shoots of PBW343. Reduced state of ascorbate was increased under both G and S in shoots but decreased in roots of C306. Maintainence of reduced state of ascorbate under S involved ABA biosynthesis in both cultivars. Roots of C306 showed toxicity symptoms under G and S where there were concomitant increases in dehydroascorbate, malondialdehyde, H2O2 and decreases reduced state of ascorbate.