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ThesisItem Open Access Studies on some aspects of bovine mastitis in Kangra valley of Himachal Pradesh(CSKHPKV, Palampur, 2010) Kaushik, Manoj; Pal, B.ThesisItem Open Access STUDIES ON SOME ASPECTS OF BOVINE MASTITIS IN KANGRA VALLEY OF HIMACHAL PRADESH(CSKHPKV, Palampur, 2010-06-22) Kaushik, Manoj; Pal, B.Mastitis is the most serious disease of lactating animals especially in high yielders. In India, losses due to mastitis amounting to the tune of rupees 2809.32 crores per annum were reported. The present investigation was carried out for a period of 1 year and 3 months. A total of 60 animals (54 cows and 6 buffaloes) comprising of 98 quarters, suffering from various types of mastitis, were included in this study. Clinical appraisal of mastitic cows and buffaloes; various direct and indirect tests viz., Strip cup test, MCMT, BTB indicator card method, WST and SCC were applied for the diagnosis. The incidence was also recorded. Microbial cultures of milk samples from affected quarters and in vitro drug sensitivity were also done. The status of micronutrients (zinc and copper), Vitamin E and acute phase proteins viz., ceruloplasmin, fibrinogen and albumin were estimated at pre and post- treatment. Treatment was done either with appropriate antibiotic alone or in combination with either tri-sodium citrate or zinc sulphate or vitamin E and selenium as per treatment schedule assigned. The overall incidence of the disease was observed to be 12.83% in College Veterinary Clinics & 6.94% in University Livestock Farm during the study period. On the basis of animals affected, the maximum incidence of mastitis was observed in the sub-acute form (46.67%) followed by acute (18.33%); whereas on quarters basis, the highest incidence was observed in sub-acute (44.90%) followed by acute (18.36%). Highest incidence was observed in crossbred (75.00%) and it was in the 4 th parity (38.33%). High yielders (30.00%) in the early stage of lactation (60.00%) and in rainy season (40.00%) were more prone to mastitis. Besides, left side quarters (60.20%) especially the fore quarters (51.02%) were mainly affected.SCC was significantly higher (p<0.01) in all types of mastitic milk. Staphylococcus spp. (58.06%) was the major isolate followed by Streptococcus spp. (19.36%), E.coli (12.90%), Bacillus spp. (5.38%) and Pseudomonas spp. (4.30%) associated with mastitis. Serum level of ceruloplasmin and plasma level of fibrinogen were found significantly higher (p<0.01) whereas albumin decreased (p<0.01) in mastitic animals and the mean values of these parameters became almost normalized at post-treatment. Zinc concentration was decreased but copper concentration was increased and Vitamin E was also decreased in mastitic animals. These values almost normalized at post-treatment. Clinically, intramammary administration of cefoperazone alongwith parenteral administration of Vit. E & Se was most effective in mastitis.