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Subject: Veterinary Public Health × Author: Neena Kumari × End date: 2020 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STUDIES ON INCIDENCE OF AEROMONAS SPP. IN READY-TO-EAT FOODS, BEVERAGES AND WATER FROM DIFFERENT TOURIST PLACES OF HIMACHAL PRADESH
    (CSKHPKV, Palampur, 2018) Neena Kumari; Panda, A.K.
    The present study was designed with the aim to determine the incidence of Aeromonas species in ready-to-eat foods, beverages and water from different tourist places of Himachal Pradesh. A total of 220 samples; RTE milk products (n=80), RTE meat/fish products (n=40), beverages (n=40) and water (n=60) were collected from 12 different tourist places. In addition, 50 stool samples from hospitalized patients were also screened. Aeromonas isolates were characterized by cultural and biochemical methods and reconfirmed by amplifying 16S rRNA (953 bp) through polymerase chain reaction (PCR). Enterotoxin genes alt (encoding heat-labile cytotonic enterotoxin), ast (encoding heat-stable cytotonic enterotoxin) and act/hlyA/aer (encoding cytotoxic/β-haemolysin/aerolysin) complex present in characterized Aeromonas isolates were detected by PCR. A total of 20 Aeromonas spp. isolates were recovered from tested samples; 16 from RTE foods, beverages, water and 4 from human stool samples. RTE milk products had highest level of contamination (n=12/80, 15%) followed by beverages (n=3/40, 7.5%) and water (n=1/60, 1.67%). These isolates were identified as A. hydrophila (n=7/20, 35%), A. sobria (n=6/20, 30%), A. schubertii (n=4/20, 20%) and A. veronii (n=3/20, 15%). All Aeromonas isolates carried alt and 25 per cent were positive for act/hlyA/aer complex. None of the isolates was carrying ast. High level of susceptibility was observed for antibiotic classes; nitrofurans, phenicols and tetracyclines followed by quinolones and aminoglycosides. However, all the isolates were resistant to ampicillin. Nine (45%) isolates were multidrug resistant. Highest number of MDR isolates were recovered from RTE milk products (25%) followed by stool samples (15%) and beverages (5%).