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20171
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Subject: Veterinary Public Health × Author: Kashyap, Ishan × Start date: 2000 ×
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    ThesisItemOpen Access
    DETECTION OF BUFFALO SPECIES IN MEAT AND MEAT PRODUCTS EMPLOYING SEROLOGICAL AND DNA BASED TECHNIQUES
    (CSKHPKV, Palampur, 2017-10-16) Kashyap, Ishan; Khurana, S. K.
    The aim of the present study was evaluation of serological and DNA based methods for detection of buffalo meat adulteration in meat and meat products. The AGID and buffalo specific PCR assay using two primer pair based on mitochondrial d-loop and 16S rRNA was employed for detection of buffalo species successfully. The serological methods i.e. AGID, the proteins were extracted from different tissues and then utilized for immunization into rabbit to produce antibodies. Antibodies were reacted for antigen detection using agarose gel immunodiffusion. The results showed serological assay can detect presence of buffalo proteins after extraction and react optimally at a distance of about 7-15 mm. However, further more and specific studies are required for immunological based study for final conclusion for authentic detection of buffalo meat. The buffalo species PCR assay was found to be specific and authentic for detection of buffalo meat adulteration in meat and meat products processed under different processing and heating conditions without any effect in less than 1 percent level of admixing with other meat species used in this study. The PCR assay were found to be specific and repeatable each and every time and can be useful tool for quality assurance of food products containing meat as ingredients. The market survey and laboratory analysis based on the buffalo specific PCR assay revealed that no adulteration of meat and meat products with buffalo species during the study periods.