Thumbnail Image

Theses

Browse

20151
Reset filters
Subject: Veterinary Physiology × Author: Chandel, Sumit × Start date: 2007 × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    STUDY ON EXPRESSION OF ANTIMICROBIAL PROTEINS IN BOVINE MILK
    (CSKHPKV, Palampur, 2015-08) Chandel, Sumit; Singh, Geetanjali
    AMPs are host defense molecules and important part of the innate immunity in animals. This study was carried out to investigate and compare the expression of AMPs particularly, cathelicidins viz CATHL1, CATHL2, CATHL3 and CATHL4 in the milk of Jersey crossbred and desi pahari cows and also to compare the expression with somatic cell count. For this, 40 milk samples of Jersey crossbred cows and 10 milk samples of desi pahari cows were collected and evaluated for somatic cell counts, MCMT, BTB pH indicator test and pH. Based primarily on varying somatic cell counts, milk samples were further selected for studying the expression of these AMPs. Liver and udder tissue samples of Jersey crossbred cow were also collected for studying the expression of these AMPs. gDNA was isolated from liver tissue sample of Jersey crossbred cow. Pairs of CathL primers viz CathL1, CathL2, CathL3 and CathL4 were tested initially on gDNA. Total RNA was isolated from the liver and udder tissue samples of Jersey crossbred cow and the selected milk samples of Jersey crossbred and desi pahari cows. cDNA was then prepared from total RNA for studying the expression of these AMPs. Expression of CATHL genes viz CATHL1, CATHL2 and CATHL3 was found in liver tissue sample of Jersey crossbred cow but no expression of CATHL4 gene was detected in liver tissue sample of same Jersey crossbred cow. Expression of CATHL2 gene was found in udder tissue sample of Jersey crossbred cow but expression of CATHL1, CATHL3 and CATHL4 genes was not found in udder tissue sample of same Jersey crossbred cow. Expression of CATHL1, CATHL2, CATHL3 and CATHL4 genes was not found in the selected milk samples of Jersey crossbred and desi pahari cows. The non-expression of these AMPs might be due to either insufficient amount of mRNA specific to these proteins or alternative splicing.