Loading...
Theses
Browse
1 results
Search Results
ThesisItem Open Access STUDIES ON IN VIVO EFFECT OF PLANT BASED EXTRACT AGAINST EXPERIMENTAL SALMONELLOSIS(CSKHPKV, Palampur, 2017-06-29) Gautam, Hemlata; Asrani, R.K.The present study was planned with a view to observe the effects of methanolic extract of roots of the plant Saussurea lappa on growth, mortality, clinical signs, biochemical and pathological changes in mice infected with a local isolate of Salmonella enterica serovar Typhimurium 4,12:i:1,2.The LD50 dose of S. Typhimurium was determined in a pilot experiment which was found to be 1.35x103 cfu ml-1.Two hundred mice were randomly divided into 7 groups, Group 1:plain control, Group 2: plant control with methanolic extract of Saussurea lappa @2000mg, Group 3: S. Typhimurium infection alone, Group 4: S. Typhimurium infection with Ciprofloxacin @200mg/kg, Group 5: S. Typhimurium infection with plant extract @200mg, Group 6: S. Typhimurium infection and plant extract @1000mg, Group7: S. Typhimurium infection and plant extract @2000mg.The mice in group 1, 2, 3 and 4 served as control. The methanolic extract of Saussurea lappa was given in drinking water to mice, 4 days before the infection and continued up to 10th of experiment. Results of the present study showed that mice of S. Typhimurium infected group exhibited clinical signs of ruffled hair coat, laboured breathing, staggering gait, hunched position, soiled anal area, soft faeces, reluctant to move and discharge from the eyes. ALT activity was found to increase, whereas total protein and albumin concentration decreased in all the infected groups as compared to the control groups. Values of creatinine was non-significantly different except in Ciprofloxacin group at 7DPI. Biochemical parameters in case of the combination groups were lower as compared to the plain infection group. Grossly group 3 animal showed hepatomegaly, hepatic necrosis, liver paleness and splenomegaly. However, in the combination groups the gross lesions of less intensity were observed. Microscopically, the liver of the mice of group 3 revealed severe necrotic foci, inflammatory cell infiltration, dilatation of sinusoids, vacuolar changes and increased cytoplasmic granularity. Similarly, in this group spleen showed congestion, necrotic foci, lymphoid depletion, granulocyte infiltration and RE cell hyperplasia. At 3 and 7 DPI, maximum lesions intensity scores were obtained. Similar changes were noticed in the combination groups (group 5, 6 and 7), but the changes were less severe in intensity as compared to the plain S. Typhimurium infected group. Histopathologically liver and spleen showed preservation of hepatocytes and hepatic cord structure and white pulp hyperplasia in plant treated groups indicated its antimicrobial, hepatoprotective and immunomodulatory effect. S. Typhimurium found to be highly pathogenic and produced significant hepatic lesion in mice model during in vivo experimentation.