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Theses

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2019 - 20203
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Subject: Veterinary Gynaecology and Obstetrics × Start date: 2018 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    EVALUATION OF INTRA-VAGINAL PROGESTERONE RELEASING INSERT IN REPEAT BREEDER COWS
    (CSHHPKV Palampur, 2020-10-05) CHANDEL, ANSHUL; Singh, Madhumeet
    The study was carried out on 43 repeat breeder cows selected from Teaching Veterinary Clinical Complex and Instructional livestock farm complex Chaudhary Sarwan Kumar Himachal Pradesh Agricultural University, Palampur along with nearby field veterinary institutions for the evaluation of controlled internal drug release (CIDR) insert. The selected cows were divided into four groups viz. Group 1 (G1; n=12) CIDR-PGF2α synchronization protocol and post-insemination CIDR insertion from day 4 to 13, Group 2 (G2; n=13) CIDR-PGF2α synchronization protocol and post-insemination CIDR insertion from day 12 to 21, and Group 3 and 4 control (G3 and G4; n=9 each) in which no treatment was given to cows and artificial insemination (AI) was done on the day of spontaneous estrus. Blood samples were collected at the time of post-insemination CIDR insertion, 72 h after CIDR insertion and 48 h after CIDR removal. Effect of treatment on follicular growth and corpus luteum development was assessed by trans-rectal ultrasonography (TRUS). Pregnancy diagnosis was done 45 days post AI by rectal examination and TRUS. Intra-vaginal CIDR insertion led to a higher conception rate in repeat breeder cows as compared to untreated cows under control group. Estrus synchronization with CIDR based protocols resulted in significantly lower (p<0.01-0.05) diameter of corpus luteum albicans as compared to nonsynchronized (control) cows at estrus. No significant increase (p>0.05) in progesterone concentration and corpus luteum size was recorded at different days of observations following post-insemination CIDR insertion in both the treatment groups as compared to control group. Subsequently pregnant repeat breeder cows following post-insemination CIDR insertion had a non-significantly (p>0.05) higher progesterone concentration and corpus luteum size at different days of observations in both the treatment and control groups as compared to non-pregnant cows. Also, mean pre-ovulatory follicular diameter was found to be non-significantly higher (p>0.05) in subsequently pregnant as compared to non-pregnant cows in treatment and control groups.
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    ThesisItemOpen Access
    ETIO-PATHOLOGY AND MANAGEMENT OF UTERINE TORSION IN BOVINES OF HIMACHAL PRADESH
    (CSK Himachal Pradesh Krishi Vishvavidyalaya Palampur, 2019) Abrol, Ankur; Singh, Madhumeet
    The present study was planned with the objectives of recording of incidence of uterine torsion in cattle and buffaloes of Himachal Pradesh and to determine the success rate of different procedures adopted to treat uterine torsion. The study was conducted in two parts. First part comprised of field survey of cases presented under field conditions of Himachal Pradesh and conducted through questionnaires. Second part comprised of recording of clinical observations, obstetrical interventions, haemato-biochemical and Doppler sonographic studies of middle uterine artery of torsion affected bovines presented in Teaching Veterinary Clinical Complex (TVCC) of college of Veterinary & Animal Sciences, Palampur, Himachal Pradesh. In the field conditions of Himachal Pradesh buffaloes were more commonly presented with uterine torsion as compared to cows and right side and post cervical torsion was encountered more frequently as compared to left side and pre cervical uterine torsion. Modified Schaffer’s method was used more often than Caesarean section under field conditions to correct the condition and has a better dam and calf survival rate as compared to Caesarean section method. Among the uterine torsion affected bovines presented in TVCC, haematological analysis of uterine torsion affected cows and buffaloes showed a significant decrease (p<0.05) in haemoglobin and lymphocyte concentration while there was a significant increase (p<0.05) in granulocyte and cortisol concentration as compared to eutocial cows and buffaloes, respectively. Resistance index was significantly higher (p<0.05) in middle uterine artery ipsi-lateral to uterine torsion affected cows as compared to eutocial cows. Blood flow volume and blood vessel diameter of ipsi-lateral middle uterine artery was significantly reduced (p<0.01) in uterine torsion affected cows and buffaloes as compared to the eutocial cows and buffaloes, respectively. Significantly higher blood flow volume on TAMAX basis (p<0.01; p<0.05) was discernible in middle uterine artery ipsi-lateral to the gravid horn as compared to the contra-lateral artery in normal eutocial cows and buffaloes.
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    ThesisItemOpen Access
    ULTRASONOGRAPHIC MONITORING OF EARLY PREGNANCY AND EMBRYONIC MORTALITY IN CATTLE
    (CSK Himachal Pradesh Krishi Vishvavidyalaya Palampur, 2019) Mahajan, Mohit; Singh, Madhumeet
    The study was conducted at Instructional Livestock Farm Complex of CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Himachal Pradesh. Trans-rectal ultrasonography of the genital tract and the middle uterine arteries was performed in twenty-nine Jersey and Jersey crossbred cows (N=29) using portable ultrasound machine with a linear rectal probe at frequency 7.5 MHz, in brightness (B-mode), color Doppler and spectral modes from day 21 to day 42 post insemination at weekly intervals. Diagnosis of the pregnancy was based on sonographic visualization of a viable embryo within uterine horns. Cows with embryonic mortality were diagnosed on the basis of embryonic debris and remnants. Whereas, cows diagnosed as non-pregnant during ultrasonographic examination were only scanned up to day 28 post-insemination. Spectral Doppler analysis of the middle uterine artery (MUA) for determination of pregnancy and embryonic mortality as performed on Day 21. Parameters such as Pulsatality index (PI), Resistivity index (RI), Vascular Perfusion Index (VPI), Mean blood flow volume (Mean BFV), Maximum blood flow volume (Maximum BFV), Time Average Mean Velocity (TAMEAN), Time Average Maximum Velocity (TAMAX), Cross sectional area of MUA and Systole-Diastole ratio were calculated. In pregnant cows, sexing of the foetus was done on Day 60 of gestation, based on migration of the genital tubercle. Out of 29 cows taken for the study, 20 were found pregnant and 9 were non-pregnant. Among 20 pregnant cows, 5 were recorded to undergo embryonic mortality subsequently. Ultrasonography was found to be an efficient tool for diagnosis of pregnancy as early as 21 days with Corpus luteum parameters and with visualization of embryo as early as 28 days of gestation. Pregnant cows were diagnosed with sonographic visualization of embryo proper within an anechoic uterine horn. Whereas, cows with embryonic mortality revealed, reduced intra-uterine allantoic fluid, with hyperechoic free-floating structures, representing embryonic debris and remnants and distorted allanto-chorionic membrane within the uterine horn. Spectral Doppler ultrasonography of middle uterine artery was also found to be a suited tool for non-invasive accurate diagnosis of pregnancy, non-pregnancy and embryonic mortality. The overall incidence of embryonic mortality evident on ultrasonographic examination was 25 per cent, with 15 per cent embryonic mortality between Day 21-28 and 10 per cent between Days 28-35 of gestation. The percentage of male and female foeti were recorded to be 57.14 and 42.86 per cent, respectively at Day 60 of gestation.