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2018 - 201912020 - 20221
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Subject: Genetics and Plant Breeding × Author: Thakur, Garima ×
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    ThesisItemOpen Access
    AMMI biplot analysis and phenotypic stability for seed yield and its component traits in linseed (Linum usitatissimum L.) under conventional and natural production systems
    (palampur, 2022-04-29) Thakur, Garima; Paul, Satish
    The present investigation entitled, “AMMI biplot analysis and phenotypic stability for seed yield and its component traits in linseed (Linum usitatissimum L.) under conventional and natural production systems” was undertaken during rabi 2019-20 and rabi 2020-21 to study the GE interactions for thirty linseed genotypes comprising of advanced breeding lines, elite cultivars and exotic lines across four different locations in Himachal Pradesh with varying altitudes viz., Palampur Bajaura, Kangra and Dhaulakuan with two production systems viz., conventional and Zero Budget Natural Farming (ZBNF) at each location. Therefore, the genotypes were evaluated over a total of sixteen environments (sites-years-production systems) for different yield and component traits. Stability analysis was carried out using Eberhart and Russell and AMMI model. Pooled analysis of variance as per both the models revealed significant differences among the genotypes, environments and GE interaction.As per Eberhart and Russell (1966) model the genotype identified as stable for seed yield was Surbhi. None of the genotypes were found stable for primary branches per plant, secondary branches per plant, capsules per plant, 1000 seed weight and oil content. Whereas, as per AMMI model stable genotypes were identified for all the traits. The most stable genotypes identified with respect to seed yield per plant were Giza-7, KL-278, Him Alsi-2, Janki and KL-279 and the most promising genotypes for oil content were Nagarkot, KL-257, KL-269, Him Alsi-1 and KL-279. Genotypes identified stable as per both the models were Him Alsi-2 for plant height, technical plant height and seeds per capsule, KL-285 and Nagarkot for plant height and Himani for harvest index. Genotypes showing specific adaptations were also identified. For site Palampur genotype Giza-8, for location Bajaura Him Palam Alsi-1(KL-241) and KL-311, for Kangra location KL-317 and Surbhi; for site Dhaulakuan genotypes KL-236, KL-315 and KL-284 showed specific adaptation under conventional system. However, under natural farming system for site Palampur genotypes Baner and KL-285, for site Bajaura genotype KL-284; genotypes KL-257 and KL-285 for location Kangra and Bhagsu, Nagrakot, Him Palam Alsi-2 (KL-263) and KL-284 for site Dhaulakuan showed specific adaptation. However, cross-overs were observed across years. Therefore, further evaluation for more years is recommended. Genotype KL-284 showed consistensy in its rank at location Bajaura under both production systems and hence, could be recommended for conventional as well as natural production systems with respect to site Bajaura. In terms of the environments, natural production system was observed as unfavourable environment for seed yield due to its consistent poor performance across years in comparison to the conventional system. It was also identified as poor discriminating environment. Genotypes KL-314 and Him Palam Alsi-2 (KL-263) were found to be resistant in all four locations for both powdery mildew and rust under epiphytotic conditions
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    ThesisItemOpen Access
    STUDIES ON MUTAGENIC EFFECTIVENESS AND EFFICIENCY OF ETHYL METHANE SULPHONATE (EMS) MUTAGEN IN LINSEED (Linum usitatissimum L.)
    (CSKHPKV, Palampur, 2018-07-24) Thakur, Garima; Paul, Satish
    The present investigation entitled, “Studies on mutagenic effectiveness and efficiency of ethyl methane sulphonate (EMS) mutagen in linseed (Linum usitatissimum L.) was undertaken to assess the mutagenic effects of mutagen EMS. The experimental material comprised of two linseed varieties viz., Him Alsi-2 and Kangra Local, seeds of which were treated with eight (0.10%, 0.20%, 0.30%, 0.40%, 0.50%, 0.60%, 0.70%, 0.80%) different concentrations of EMS to generate M1 generation. On the basis of survival percentage in M1 generation under lab conditions the LD50 dose for both varieties was estimated i.e. 0.70 per cent for Him Alsi -2 and 0.60 per cent for Kangra Local. Plants harvested from only three concentrations (one higher and one lower dose to LD50) in M1 were carried forward to M2 generation. Data were recorded for various qualitative and quantitative traits along with reaction to diseases viz., powdery mildew and rust on individual plant basis. Mutagen EMS was effective and efficient in producing chlorophyll mutations and viable macromutations in M2 generation. EMS was also found effective in producing variability in range and mean values for different quantitative characters for which significant differences were obtained in both positive and negative direction. The EMS concentrations showing maximum effectiveness and efficiency in both varieties were different indicating varietal sensitivity towards the mutagen. The most effective dose for producing chlorophyll mutations between both varieties was 0.60 per cent and the most efficient was 0.50 per cent. For viable as well as total macromutations most effective concentration recorded was 0.70 per cent and 0.60 per cent as the most efficient. The dose found effective was not necessarily found efficient in producing mutations in both varieties.