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ThesisItem Open Access Genetic studies in Lolium-Festuca species and development of Lolium-Festuca hybrids(palampur, 2023-02-23) Kumari, Anjali; Sood, V.K.The present investigation entitled “Genetic studies in Lolium-Festuca species and development of Lolium-Festuca hybrids” was undertaken to assess genetic variation among four Lolium and 10 Festuca genotypes for various morphological and quality traits viz., plant height, leaf length, leaf width, tillers per plant, leaf area, green forage yield per plant, dry matter yield per plant, acid detergent fiber, neutral detergent fiber, crude protein content, and crude protein yield per plant, to develop intergeneric hybrids between Lolium and Festuca species and to identify good general combiners using polycross breeding in 10 fescue grass genotypes under field and polyhouse conditions at CSKHPKV, Palampur. A drought stress of 10 days was given to 45 days old plants to evaluate for various physiological traits such as root length, shoot length, root weight, shoot weight, root: shoot, leaf relative water content, chlorophyll a, b, a/b, total chlorophyll, proline content under well watered and drought stress conditions. On the basis of mean performance during drought stress conditions, among Festuca genotypes, Hima-3, Sel-63, Hima-15, Sel-88 and EC178182 were observed as drought tolerant genotypes whereas among Lolium genotypes, Palam ryegrass was observed as drought tolerant one. Pooled analysis of variance over cuts revealed significant differences among genotypes for all the traits studied viz., plant height, leaf length, leaf width, tillers per plant, leaf area, GFY, DMY, ADF, NDF, CP content and CPY. PCV and GCV were found to be high to moderate for most of the traits. High heritability coupled with high genetic advance, correlation studies and path analysis showed that selection for plant height, leaf length, tillers per plant, GFY and DMY would be effective. On the basis of polycross analysis, genotype Hima-3, Hima-1, Sel-71, Hima-15, EC-178182 and EC178184 were found to be the good general combiners for tillers per plant, GFY, DMY, ADF, NDF, CP%, CPY, leaf length, leaf width, plant height, leaf area and various physiological parameters that could further be used for the development of synthetics in fescue grass. Out of 12 crosses between four Lolium and three Festuca genotypes, only two intergeneric hybrids have been successfully developed which could further be used for analysis in Lolium- Festuca breeeding programmes.
