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Subject: Biochemistry × Author: THAKUR, NITASHA × Start date: 2003 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    STUDIES ON THE EFFECT OF METSULFURON METHYL ON BIOSYNTHETIC PATHWAY OF WHEAT AND ITS FATE IN SOIL
    (Department of Chemistry and Biochemistry, 2016-01) THAKUR, NITASHA; Sharma, Neelam
    A field experiment was laid out in randomized block design (RBD) consisted of four treatments viz. metsulfuron methyl 2 g ha-1, metsulfuron methyl 4 g ha-1, metsulfuron methyl 8 g ha-1 and control to study the effect of metsulfuron methyl on biosynthetic pathway of wheat and its degradation behaviour in the Department of Agronomy, Forages and Grassland Management, CSK HPKV, Palampur during Rabi 2013-14 and Rabi 2014-15. Wheat plant and soil samples were collected at 0 (4 hr), 1, 3, 7, 10, 15, 30, 45, 60, 75 and 90 days after herbicide application for biochemical and degradation studies. A significant decrease in biochemical attributes of wheat leaves i.e. acetolactate synthase activity and total soluble protein was observed during both the years of study upto 10 days after metsulfuron methyl application @ 2 g ha-1, 4 g ha-1 and 8 g ha-1. Whereas, amino acid content in wheat leaves was not influenced very distinctly with applied treatments. However, in general an increase in amino acid content was noticed in metsulfuron methyl treatments over control. Over the period of time, total chlorophyll and total sugar content also increased significantly in wheat leaves. Quality indices (protein, sugar, amino acid and gluten content) of wheat grain increased significantly with applied metsulfuron methyl treatments. The degradation data generated in the present investigation during both years indicated that higher dose of metsulfuron methyl i.e. 8 g ha-1 persisted in soil upto 45 days after herbicide application. The degradation of herbicide was rapid and more than 90 per cent of applied metsulfuron methyl in soil dissipated within 15 days of herbicide application. The logarithmic plots of herbicide concentration at different doses versus time fitted first order kinetics decay curves during both years. At the time of harvest, the metsulfuron methyl residues were non-detectable in wheat straw and wheat grain.