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2011 - 20163
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Subject: Biochemistry × Author: Rana, Anita × Start date: 2007 ×
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    ThesisItemOpen Access
    Studies of biochemical constituents and fatty acids profile of pecan nut
    (CSKHPKV, Palampur, 2011) Rana, Anita; Singh, Nageswer
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    ThesisItemOpen Access
    CHLOROTIC LEAF SPOT DISEASE OF ARKA (Calotropis gigantea): MOLECULAR CHARACTERIZATION OF THE CAUSAL AGENT AND EFFECT ON PHYTOCHEMICAL CONSTITUENTS
    (CSKHPKV, Palampur, 2016-01) Rana, Anita; Singh, Nageswar
    ABSTRACT The present work entitled, ‘‘Chlorotic Leaf Spot Disease of Arka (Calotropis gigantea): Molecular Characterization of the Causal Agent and Effect on Phytochemical Constituents’’ is focused on the effect of disease on active phytochemical constituents in leaves of Calotropis gigantea and the molecular characterization of the causal agent. Based on the nature of symptoms i.e. systemic brilliant chlorotic to yellow spots on the leaves of C. gigantea may be the infection due to virus. Phytochemical constituents viz. antioxidant activity, quinones, flavonoids, total phenols, ascorbic acid, PPO activity have higher values in infected leaves whereas total chlorophyll and carotenoids had lower values in infected leaves. In case of PPO activity it was found maximum at same time interval for both of the samples. However, PPO activity was found higher in infected sample and lower in healthy sample. Molecular characterization studies revealed the presence of virus particles on the basis of results obtained from Virus Like Particle (VLP) purification and electron microscopy. Some icosahedral, enveloped entities of approximately 50-60 nm were observed which proved the presence of virus. The sequencing results showed similarity to insect infected virus Nucleopolyhedrosis (OpMNV) and Paramecium bursaria chlorella virus (PBCV) from ocean virome studies which were bacterial in origin. Irrespective of different strategies performed for cloning, ligation and PCR the sequencing results were similar. However, these viruses have not been reported in plants. Thus results are indicative of presence of a novel virus or new DNA which needs to be further investigated.
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    ThesisItemOpen Access
    CHLOROTIC LEAF SPOT DISEASE OF ARKA MOLECULAR CHARACTERIZATION OF THE CAUSAL AGENT AND EFFECT ON PHYTOCHEMICAL CONSTITUENTS
    (DEPARTMENT OF CHEMISTRY AND BIOCHEMISTRY, 2016-01-23) Rana, Anita; Singh, Nageswer
    ABSTRACT The present work entitled, ‘‘Chlorotic Leaf Spot Disease of Arka (Calotropis gigantea): Molecular Characterization of the Causal Agent and Effect on Phytochemical Constituents’’ is focused on the effect of disease on active phytochemical constituents in leaves of Calotropis gigantea and the molecular characterization of the causal agent. Based on the nature of symptoms i.e. systemic brilliant chlorotic to yellow spots on the leaves of C. gigantea may be the infection due to virus. Phytochemical constituents viz. antioxidant activity, quinones, flavonoids, total phenols, ascorbic acid, PPO activity have higher values in infected leaves whereas total chlorophyll and carotenoids had lower values in infected leaves. In case of PPO activity it was found maximum at same time interval for both of the samples. However, PPO activity was found higher in infected sample and lower in healthy sample. Molecular characterization studies revealed the presence of virus particles on the basis of results obtained from Virus Like Particle (VLP) purification and electron microscopy. Some icosahedral, enveloped entities of approximately 50-60 nm were observed which proved the presence of virus. The sequencing results showed similarity to insect infected virus Nucleopolyhedrosis (OpMNV) and Paramecium bursaria chlorella virus (PBCV) from ocean virome studies which were bacterial in origin. Irrespective of different strategies performed for cloning, ligation and PCR the sequencing results were similar. However, these viruses have not been reported in plants. Thus results are indicative of presence of a novel virus or new DNA which needs to be further investigated