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ThesisItem Open Access Mineral profiling with standardization of acid digestion procedure of locally available milk in cow and buffalo.(CSK HPKV, Palampur, 2023-02-16) Kaushal, Karan; Katoch, ShivaniThe study was conducted to perform mineral profiling with standardization of acid digestion procedure of locally available milk in cow and buffalo breeds. For this purpose, whole milk samples (n=220) from Jersey, Sahiwal, Zebu and Murrah breeds were collected from different districts of H.P namely Kangra, Hamirpur and Mandi. Each milk sample collected was digested with three different methods viz. dry ashing, wet (open vessel) and microwave based. Acid recipes T0 (1:1, HCl 5mL), T1 (7:3, 0.7mL HNO3 + 0.3mL H2O2) and T2 (4:1:1, 0.8mL HNO3 + 0.2mL H2O2 + 0.2 mL HClO4) were used for acid digestion of milk samples in all the digestion techniques. The mineral elements (Ca, Fe, Zn, Cu, Mn and P) in acid digested milk were analysed in Atomic Absorption Spectroscope (AAS) and then compared on the basis of the digestion method and acid recipe used. Significantly (P<0.05) higher release and recording of elements viz. Ca (162.29 ppm), Fe (8.46 ppm) and Cu (0.741 ppm) were recorded with acid recipe T2 employing microwave digestion method and for mineral elements Zn (13.11 ppm) and Mn (0.430 ppm), acid recipe T2 by dry ashing exhibited significantly (P<0.05) higher mineral content. Further, P (0.32mg/100mL) content recorded was significantly (P<0.05) higher for acid recipe T1 in dry ashing digestion technique. Analysis of the milk samples of different breeds of cow and Murrah buffalo revealed significantly (P<0.05) higher content of Ca (215.05 ppm), Cu (0.970 ppm), Mn (0.471 ppm) and P (0.33 mg/100mL) in Murrah buffalo while the trace elements Fe (11.04 ppm) and Zn (15.5 ppm) were recorded to be significantly (P<0.05) higher in milk of Sahiwal breed.
