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20191
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Subject: Animal Genetics and Breeding × End date: 2020 × Start date: 2019 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    MOLECULAR GENETIC CHARACTERIZATION OF CERTAIN GROWTH RELATED GENES AND ANALYSE THEIR ASSOCIATION WITH SOME GROWTH TRAITS IN GADDI GOATS.
    (CSKHPKV, Palampur, 2019-07-22) Gitanjali; Thakur, Y.P.
    This study was undertaken for molecular genetic characterization of IGF-1 gene and four fragments of GH gene in Gaddi goats and analyse their association with growth traits. Blood samples from 63 genetically unrelated animals of Gaddi goat breed were taken from the migratory flocks under AICRP and Gaddi goat unit of CSKHPKV, Palampur and subjected to DNA isolation using Phenol Chloroform extraction technique. 363, 422, 116, 389 and 181 bp amplicons were generated on amplification of target loci of IGF-1 and GH gene. IGF-1 gene, GH1 and GH2 fragments were analysed using PCR-RFLP (HaeIII RE) that revealed two variants (AB and BB) for IGF-1 and GH2 whereas, three variants (AB, BB and AA) were revealed for GH1 fragment. GH3 and GH4 fragments were subjected to PCR-SSCP that detected three genotypes (AB, BB and AA) for GH3 however, GH4 was found to be monomorphic. Significant association (P≤ 0.05) was detected for GH1 with 9- month body weight, GH2 with 9 and 12 months’ heart girth and GH3 with 6-month body weight, body height and body length, respectively. Allele frequencies for ‘A’ allele were 0.25, 0.53, 0.62 and 0.67 and 0.75, 0.47, 0.38 and 0.33 for ‘B’ allele. The PIC values revealed median level of polymorphism in studied population. From the present study, it was concluded that SNPs and their association with some body measurements may be useful in selecting goats for higher growth traits.