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Theses

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2015 - 20193
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Subject: Animal Genetics and Breeding × End date: 2020 × Start date: 2015 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    MOLECULAR GENETIC CHARACTERIZATION OF CERTAIN GROWTH RELATED GENES AND ANALYSE THEIR ASSOCIATION WITH SOME GROWTH TRAITS IN GADDI GOATS.
    (CSKHPKV, Palampur, 2019-07-22) Gitanjali; Thakur, Y.P.
    This study was undertaken for molecular genetic characterization of IGF-1 gene and four fragments of GH gene in Gaddi goats and analyse their association with growth traits. Blood samples from 63 genetically unrelated animals of Gaddi goat breed were taken from the migratory flocks under AICRP and Gaddi goat unit of CSKHPKV, Palampur and subjected to DNA isolation using Phenol Chloroform extraction technique. 363, 422, 116, 389 and 181 bp amplicons were generated on amplification of target loci of IGF-1 and GH gene. IGF-1 gene, GH1 and GH2 fragments were analysed using PCR-RFLP (HaeIII RE) that revealed two variants (AB and BB) for IGF-1 and GH2 whereas, three variants (AB, BB and AA) were revealed for GH1 fragment. GH3 and GH4 fragments were subjected to PCR-SSCP that detected three genotypes (AB, BB and AA) for GH3 however, GH4 was found to be monomorphic. Significant association (P≤ 0.05) was detected for GH1 with 9- month body weight, GH2 with 9 and 12 months’ heart girth and GH3 with 6-month body weight, body height and body length, respectively. Allele frequencies for ‘A’ allele were 0.25, 0.53, 0.62 and 0.67 and 0.75, 0.47, 0.38 and 0.33 for ‘B’ allele. The PIC values revealed median level of polymorphism in studied population. From the present study, it was concluded that SNPs and their association with some body measurements may be useful in selecting goats for higher growth traits.
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    ThesisItemOpen Access
    MICROSATELLITE BASED MOLECULAR GENETIC CHARACTERIZATION OF CHEGU–PASHMINA GOAT BREED
    (CSKHPKV, Palampur, 2015) SHARMA, ASHISH; Thakur, Y.P.
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    ThesisItemOpen Access
    GENETIC STUDIES ON SOME ECONOMIC TRAITS IN RAMBOUILLET x GADDI (HIMTAL MERINO) CROSSBRED SHEEP UNDER SUBTEMPERATE CONDITIONS
    (CSKHPKV, Palampur, 2016-02) Thakur, Shalini; Thakur, Y.P.
    ABSTRACT A crossbred sheep population locally named as “Himtal Merino” with 75% Rambouillet inheritance have been evolved at Govt. Sheep Breeding Farm, Tal (Hamirpur) by crossing of Gaddi ewes with Rambouillet rams. Data on 1204 crossbred lambs for growth and wool production, 669 ewes for reproductive and 370 sheep for lifetime performance traits, progeny of 28 sires, were analysed by LSMLMW method using a fixed effect model to study mean performances and effect of some genetic and non-genetic factors on performance traits. The variance and co- variance components for genetic parameters were estimated by single trait animal model using WOMBAT software. The overall least squares means were 3.26±0.06, 16.11±0.03, 21.82±0.06 and 26.29±0.12 kg for lamb‟s weight at birth, 3, 6 and 12 months of age respectively, 141.44±0.18 g/day for pre weaning growth rate and 63.68±0.34 g/day during 3-6 months of age, 24.53±0.45 g/day during 6-12 months age and 36.64±0.38 g/day over 3-12 months of age post weaning body weight gain. The means were higher for HTM than R x G and R x RG lambs. Higher growth rate was achieved during pre weaning than post weaning stages. The mean age at first breeding and first lambing were 924.25±0.78 and 1070.51±0.48 days respectively. For wool production traits, the means for age at first shearing (AFS) was 184.62±0.14 days, shearing interval as 180.29±0.07 days, first and second annual greasy fleece were 1823.30±1.59 g and 2163.83±1.83 g respectively, second annual yield being higher than first. The mean longevity was 1210.72±26.39 days with productive life span of 945.84±26.39 days. The heritability for growth traits was low to medium being 0.19±0.05, 0.14±0.04, 0.20±0.05 and 0.06±0.03 for birth, 3, 6 and 12 months of age body weights. High heritability (0.62±0.01) was estimated for pre weaning body weight gain. For post weaning body weight gains, heritability were 0.14±0.04 for 3-6 months, 0.15± 0.04 for 6-12 months and 0.10±0.04 for 3-12 months of age. The heritability for various reproductive traits were low to moderate with estimates of 0.22±0.02, 0.02±0.10, 0.08±0.11, 0.07±0.10, 0.21±0.04, 0.31±0.06, 0.16±0.03, 0.18±0.05 for age at first breeding, age at first lambing, first lambing interval, second lambing interval, first service period, second service period, first gestation period and second gestation period respectively. For wool production traits, low heritability of 0.19±0.07 and 0.18±0.06 was estimated for age at first shearing and shearing interval and low to moderate heritability of 0.21±0.06, 0.14±0.04, 0.24±0.11, 0.23±0.09, 0.31±0.08 and 0.29±0.07 for fleece yield at first, second, third and fourth clips, first and second annual fleece yields respectively. Most genetic and phenotypic correlations among growth traits were positive and high. Positive genetic correlations were observed among various wool production traits and growth traits and wool production traits indicated scope for selection on basis of early expressed growth traits.