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  • ThesisItemOpen Access
  • ThesisItemOpen Access
    STUDIES ON GENETIC AND NON GENETIC VARIATIONS OF ANGORA RABBITS REARED IN KANGRA VALLEY OF HIMACHAL PRADESH
    (CSKHPKV, Palampur, 2010-07) SHARMA, Loopamudra; Katoch, Sanjeet
    ABSTRACT This study was conducted to evaluate the phenotypic profiles, genetic and non genetic effects on wool production and reproduction traits in 588 and 361 German Angora rabbits maintained at Angora Rabbit Breeding Farm, Kandwari, Kangra (Himachal Pradesh) from 2000 to 2009 for reproduction traits and from 2000 to 2007 for wool production traits. The data was analyzed by Harvey’s least squares method of fitting constants. The overall least squares means were 4.62 1.64 k, 30.39 0.58 d, 58.77 35.10, 169.77 30.38, 181.00 21.30, 184.73 23.49, 594.22 67.13, 591.82 193.67 and 1185.96 202.96 g respectively for litter size at birth, gestation period, wool yield at first, second, third, fourth clip, first year, second year and cumulative yield. Year and season of kindling significantly affected litter size at birth, wool yield at first, third, fourth clip and first year yield. Gestation period, wool yield at second clip, second year and cumulative yield were affected by year. Heritability estimates were 0.09, 0.29 0.030, 0, 0.312 0.021, 0.27 0.017, 0.412 0.028 and 0.116 0.013 respectively for wool yield at first, second, third, fourth clip, first year, second year and cumulative yield. Genetic correlations of wool yield at second clip with second year and cumulative yield, wool yield at fourth clip with second year and cumulative yield and first year with second year and cumulative yield were negative. Wool yield at first clip with cumulative yield, second with third clip and third with fourth clip, first year, second year and cumulative yield were zero. Wool yield at second clip with fourth clip had the highest genetic correlation (1.226 0.398). Phenotypic correlations of second clip with second year and third clip with second year yield were negative. Second year and cumulative yield had the highest significant phenotypic correlation (0.942). Winter appeared to be the most favorable season for breeding of Angora rabbits. Improvement can be achieved with proper selection and improved management practices. Pure breeding stock of Angora rabbits can be introduced to maintain superior genotypes and variability.
  • ThesisItemOpen Access
    GENETIC CHARACTERIZATION OF HILL CATTLE OF HIMACHAL PRADESH USING MOLECULAR MARKERS
    (CSKHPKV, Palampur, 2008) Kumar Mahajan, Kailash; Kaila, O.P.
  • ThesisItemOpen Access
    GENETIC STUDIES ON INHERITANCE OF PART LACTATION MILK YIELDS IN RED SINDHI X JERSEY CROSSBRED COWS
    (CSKHPKV, Palampur, 2010-06) Verma, Nishant; Thakur, Y.P.
    ABSTRACT Records on 136 Red Sindhi x Jersey crossbred cows, maintained at Dairy Farm, Himachal Pradesh Krishi Vishva Vidyalaya, Palampur for 24 years (1981-2004) were analyzed for the effect of genetic group, season of calving, period of calving and parity of cow on cumulative part lactation milk yields. Heritabilities and genetic and phenotypic correlation coefficients were also estimated for these traits. The overall least square means estimated in this study for cumulative milk yields up to 30, 60,120,240 days were 267.46 ± 10.34kg, 548.82 ±21.57kg, 1061.27 ± 40.01kg and 1877.52 ± 59.98 kg respectively. The overall average for other traits viz. 305-days milk yield, total milk yield of lactation, lactation length, peak yield, calving interval, average milk yield per day of lactation length and average milk yield per day of calving interval were 2192.26±65.12kg, 2522.87±80.48kg, 375.11±4.02 days, 459.4±35.17days,12.76±0.36kg, 6.78±0.17kg and 5.61±0.16kg respectively. Cumulative part lactation milk yield traits were only affected by parity of cow. Genetic group, season of calving and period of calving were found to have no significant effect on these traits. The other traits viz. 305-days milk yield, total milk yield of lactation, lactation length, peak yield, calving interval, average milk yield per day of lactation length and average milk yield per day of calving interval, were not affected by genetic group, season of calving and period of calving and parity of cow except 305 days milk yield which was significantly affected by parity of cow. High heritability (0.54±0.19 of 30 days yield to 0.70±0.23 of 120 days yield) and high genetic and phenotypic correlations were observed among different part lactation milk yield traits. The correlation coefficients were moderate to high between part lactation milk yield traits and complete lactation milk yield traits. The high heritability of 60 days milk yield (0.68±0.22), 120 days milk yield (0.70±0.23) and moderate heritability of peak yield (0.324±0.15) and correlations of these traits with other complete lactation milk yield traits indicates that these traits can be utilized as reliable tools for early selection of dairy cows. Low heritabilities for calving interval and lactation length indicates that the length of these traits is much influenced by management and husbandry practices, which can be improved by better management practices.
  • ThesisItemOpen Access
    GENETIC CHARACTERIZATION OF NATIVE CHICKEN OF HIMACHAL PRADESH USING MOLECULAR MARKERS
    (CSKHPKV, Palampur, 06-07-11) REEN KOUR, JAGISH; Katoch, Sanjeet
    ABSTRACT Sixty five (65) blood samples at random from birds procured from different locations of the state and maintained at CSKHPKV Poultry farm, Palampur were collected. Fifteen microsatellite markers (MCW073, MCW005, MCW004, MCW051, MCW049, MCW048, MCW001, MCW014, ADL-158, ADL-172, ADL-267, ADL-210, ADL-171, ADL-176, ADL136) which gave amplification were included in the analysis. The PCR products for different microsatellite loci were resolved on 6% denaturing (urea) polyacrylamide gels along with 50 and 100 bp DNA ladders at 40-45V. Microsatellite alleles were visualized by silver staining. The observed alleles, effective number of alleles, gene frequency, observed heterozygosity, expected heterozygosity were estimated through POPGENE software. The mean observed and effective numbers of alleles were found to be 5.87 ± 0.28. and 4.19 ± 0.17 respectively across all loci. Total 88 alleles were observed with maximum alleles (8) contributed by locus MCW073, ADL-176 & and the least alleles (4) by (ADL-171, ADL-136, MCW014). The average heterozygosity was observed to be 0.76 ± 0.01 across all loci. The average observed and expected heterozygosity 0.56 ± .03 and 0.77 ± 0.01 respectively. The observed heterozygosity (0.8900) was highest for ADL-176, and least (0.4082) for ADL-267. The Expected heterozygosity (0.8278) was highest for MCW073 and least (0.6890) least for MCW014. All the microsatellites had shown high levels of genetic variability.The screened loci were not found to be in accordance with Hardy-Weinberg equilibrium for several microsatellite frequencies. Genes ChB6 and IL-2Rγ were used to study nucleotide polymorphism related to immunocompetence The PVU-II restriction enzyme digestion of 215bp amplicon of ChB6 gene generated fragments of 215,147 & 68 bp and revealed highly polymorphic patterns. The Hph1 restriction enzyme digestion of 600bp amplicon of IL-2Rγ gene generated fragments of 465,454,134,104,42bp and revealed highly polymorphic patterns. Fragment pattern revealed all the genes have high level of polymorphism in DNA samples of Native Chicken of Himachal Pradesh.