Thumbnail Image

Theses

Browse

20201
Reset filters
Subject: Agricultural Biotechnology × Author: ANKITA × Start date: 1980 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON CRYOPRESERVATION AND REGENERATION OF IN VITRO RAISED CULTURES OF PICRORHIZA KURROA
    (CSK HPKV Palampur, 2020-09-28) ANKITA; Shashi, Bhushan
    Picrorhiza kurroa is an endangered Himalayan perennial herb and important ingredient of traditional medicinal systems of Asian region. It’s medicinal value primarily depends upon presence of picrosides in its roots. These bioactives are used for preparation of hepatoprotective phytopharmaceuticals. This herb is uprooted from wild for extraction of bioactive ingredients, which is a major reason for its depletion from natural habitat. Considering the high demand and low supply of P. kurroa population, efforts for its conservation through biotechnological tools is urgently required. In current research, cryopreservation protocol of high picroside producing cell lines of Picrorhiza kurroa were developed. P. kurroa shoot cultures incepted with 0.50 mg/L of Kn. Highest in vitro average shoot number was observed at 0.50 mg/L Kn strengthened MS medium with 3.18 shoots per shoot tip whereas 4.29 cm shoot length with 11.46 average number of leaves recorded on MS basal medium. Highest callus induction (100%) was found on MS medium augmented with 0.0125 mg/L IBA + 0.125 mg/L TDZ from leaf and root explants. Calli multiplied on the same medium with 6.89 fold increase in fresh weight. Leaf and root explants of in vivo and in vitro grown plants were analysed for identification of high metabolite producing cell lines. Among in vivo leaf and root explants, highest P-I content (5.65 %) was found in leaves whereas highest P-II content (8.37 %) was found in roots. In case of in vitro leaves and roots, leaves contained higher P-I (1.160 %) & P-II (0.280 %) content. Callus derived from leaves also showed highest total picroside content i.e 0.2 per cent, therefore, leaf derived callus was used for cryopreservation. Among all the cryopreservation methods used i.e encapsulation-vitrification, vitrification and desiccation, only encapsulation-vitrification showed 60 per cent regeneration at 15 minutes of dehydration time with PVS2 solution. Encapsulation-vitrification protocol would be useful for ex-situ conservation of P. kurroa callus cultures.