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Author: ANISH RAJAN × End date: 2015 × Start date: 2015 ×
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    ThesisItemOpen Access
    EVALUATION OF B-MODE AND COLOUR DOPPLER ULTRASONOGRAPHY FOR DETECTION OF IN VIVO OVULATION IN CANINES
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 2015-09) ANISH RAJAN
    With the objective of evaluation of B-mode and colour Doppler ultrasonography for detection of in vivo ovulation in canines, six apparently healthy, post pubertal bitches of different breeds were subjected to B-mode and colour Doppler ultrasonography daily after the detection of pro-oestrus. The study was conducted at University Veterinary Hospital, Kokkalai. Methods like vaginal cytology, vaginal pH, cervico-vaginal mucus arborisation and vaginoscopy were performed in an attempt to relate the ultrasonographic findings with the oestrous cycle stages. The mean interval between ovulation and anuclear cell index peak was 1.5 ± 0.224 days. Vaginal pH was lowest on the day of ovulation in all the bitches. Cervico-vaginal mucus arborisation and vaginal mucosal shrinkage peaked two days after ovulation. Serum progesterone estimation was done using radioimmunoassay. The mean progesterone levels at day of LH surge, day of ovulation and first day of metoestrus were 1.323 ± 0.299 ng/ml, 2.070 ± 0.454 ng/ml and 8.655 ± 0.558 ng/ml, respectively. The mean ovarian surface area increased from 1.048 ± 0.116 cm2 and 1.151 ± 0.160 cm2 , respectively, for left and right ovaries on the day of LH surge to reach 1.166 ± 0.185 cm2 and 1.205 ± 0.454 cm2, respectively, on the day of ovulation. The mean follicular diameters, when measured 3 days preceding ovulation was 0.386 ± 0.032 cm and 0.370 ± 0.048 cm for left and right ovaries, respectively and it reached maximum size on the day before ovulation to 0.501 ± 0.030 cm and 0.470 ± 0.026 cm, respectively. The increase in ovarian blood flow after the occurrence of LH surge was detected by colour Doppler ultrasonography. The mean number of follicles detected by ultrasonography was 5.33 ± 0.558. There was no statistical difference between the number of follicles detected by ultrasonography and the litter size of 5.00 ± 0.577 obtained. It was concluded that by performing daily ovarian ultrasonography in vivo ovulation could be precisely detected.