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20171
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Author: ABHILASH R. S × Start date: 1981 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    YIELD AND QUALITY OF TRANSVAGINALLY RETRIEVED OOCYTES IN NORMAL AND REPEAT BREEDING CATTLE
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2017-12-01) ABHILASH R. S; ABHILASH R. S; Metilda Joseph; Metilda Joseph
    The study was conducted to assess the efficacy of transvaginal oocyte recovery (TVOR) and to evaluate the quality of oocytes recovered from 12 normal and 12 repeat breeder crossbred cows stationed at the University Livestock Farm and Fodder Research Station, Mannuthy. Both normal and repeat breeder cows further divided into two groups of six animals each based on the TVOR frequency. Transvaginal oocyte recovery was performed at a frequency of once weekly in groups I and III and twice weekly in group II and IV for a period of two months (Group I and II- normal cows, and group III and IV- repeat breeders, six animals each). Frequency of TVOR or reproductive status of animal was not found to influence ovarian biometry. Number of small ( 9 mm) follicles were significantly lower (p ≤ 0.05) in normal and repeat breeder cows subjected to twice weekly TVOR. The number of follicles aspirated, number of oocytes retrieved, culture grade oocytes, matured oocytes, fertilized oocytes, cleaved oocytes per session and oocyte recovery rate (%) were siginicantly higher (p ≤ 0.05) in normal animals and those animals subjected to twice weekly TVOR. However, there was no significant difference between maturation rate, fertilization rate and cleavage rates between normal and repeat breeder animals and animals subjected to once and twice weekly TVOR. Evaluation of oocyte maturation rate using Hoechst 33342 and FDA also revealed that maturation rate was higher in normal breeders. No significant difference in the serum progesterone and blood urea nitrogen level were observed when normal and repeat breeder cows were subjected to TVOR at varying frequencies. A significantly higher follicular fluid progesterone concentration was observed in normal animals when compared to repeat breeders. The present study revealed that oocyte quality in repeat breeders is significantly lower than normal breeders, and TVOR at twice weekly interval was found to be an effective tool for harvesting maximum number of oocytes from crossbred cows.