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20131
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Subject: Veterinary Pathology × Author: SUVANEETH P × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    COMPARATIVE HEALING RESPONSES OF DIFFERENTLY PROCESSED DECELLULARISED BOVINE PERICARDIUM IN RAT ABDOMINAL REGENERATION MODEL
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 2013) SUVANEETH P
    The efficacy of two different protocols for the decellularisation of bovine pericardium for induced regeneration has been evaluated in this experiment. The pericardia were decellularised using a non-detergent based enzymatic protocol and a biodetergent (deoxycholic acid) based newer protocol, and was evaluated in vitro and in vivo for comparison. The in vitro characterisation was done using tensile testing, contact angle measurement and collagenase susceptibility. The in vivo implantation studies were carried out in full wall abdominal defect model in Sprague-Dawley rats. The inflammatory, immune and regenerative responses were evaluated 15, 30 and 90 days post implantation. The H&E stained sections were evaluated semi-quantitatively for the cellular and tissue responses of inflammation, collagenisation and regeneration of skeletal muscles. Skeletal muscle formation and collagenisation were further confirmed by Masson trichrome stain. The inflammatory response to the implants was chronic with mononuclear cell infiltration, characterised by lymphocytes, plasma cells and macrophages. The autograft significantly differed and found advantageous to the implants. The implants were largely accepted by all the experimental animals as shown by increased neovascularisation, fibroblast proliferation, collagen deposition and striated muscle formation. Collagen deposition in the implants was found to be of similar extend which was analysed using image analysis. The results of the study showed that bovine pericardium decellularised with deoxycholic acid treatment and bovine pericardium decellularised using nondetergent based enzymatic process elicited comparable inflammatory and good regeneration responses and there were no differences in the responses of decellularised pericardium produced by two different protocols and both the materials were found to be equally effective in inducing regeneration.