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ThesisItem Open Access Effect of biomordants on dyeability of cotton fabric with natural dye(CCS HAU, Hisar, 2020-07) Neeta; Arya, NishaIncreasing concern about environmental pollution in all sphere of life has raised grave concern and heated debates around the world about the concept of ecology and environment. In the present time, commercially viable alternative methods for preparing and finishing cotton substrates based on the use of biomordants have emerged. Keeping in view the current scenario of environmental consciousness, the present study has been planned to assess the effect of biomordant on dyeing efficacy of cotton with natural dye substituting metal based mordant and salts. Standardization of biomordanting process for two biomordants i.e. harad and heena was done on the basis of dye absorption, colour strength and wash fastness rating for different concentrations and treatment conditions. The desized and scoured fabric were pre-treated with selected two biomordants and dyed with selected natural dye. Scanning Electron Microscopic (SEM) analysis of biomordanted fabric samples. SEM analysis demonstrated plain surface of biomordant treated sample. The colour coordinates, colour strength and colour fastness of the biomordant treated dyed samples were assessed. Various mechanical, performance and functional properties of the biomordant treated dyed fabric were also tested. Comparative analysis was also done between scoured fabric treated with biomordants and dyed with natural dye. The parameter optimized for biomordanting process of scoured fabric were pre mordanting stage with 5 and 10 per cent concentration, 1:30 M: L Ratio, 6 and 4 pH, 60° and 80°C treatment temperature and 60 and 45 minutes treatment time for heena and harad, respectively. Both the biomordant treated dyed samples exhibited good (4) to very good (4/5) colour fastness properties. The results revealed that among both the biomordants treated dyed fabrics, heena treated dyed fabric showed maximum increase in bending length (2.39 cm) and heena treated dyed sample showed highest increase in elongation (29.68%), crease recovery angle (113 degree), moisture regain (10 %) and wickability (3.86cm). The heena treated and Babool bark dyed fabric showed the highest UPF value (56.89) indicating excellent protection category while in harad treated dyed fabric UPF value was (48.76). The biomordants treated dyed fabrics showed bacterial resistance against E. coli (93.35%) and S. aureus (88.07%). Thus, it is concluded that biomordants treatment enhanced the dyeing efficacy of the cotton fabric with better colour fastness properties. The biomordant treatment and Babool bark dye improved the mechanical and performance properties of cotton fabric with very good to excellent protection from UV radiation and bacterial attack. Hence, the biomordants are suitable replacement materials for cotton fabric in textile wet processing due to their positive response towards environment and no harmful effect on fabric properties.ThesisItem Open Access Evaluation of advanced wheat lines for stripe rust resistance and grain quality(CCSHAU, Hisar, 2019-08) Bhawna; Bhawna; Yadav, Neelam R.; Yadav, Neelam R.Biofortification of staple foods like wheat without any yield penalty is of utmost importance. Advanced lines introgressed with Gpc-B1 allele were used in the study. The Gpc-B1 allele co-segregated with Yr36 yellow rust resistance gene in all the advanced lines. All WH711-derived advanced wheat lines confirmed for the Yr36/Gpc-B1 allele. Seven advanced lines (WL-8-1, WL-8-2, WL-8-3, WL-8-5, WL-8-6, WL-8-7, WL-8-8) were confirmed for Yr18 while six advanced lines (WL-8-1, WL-8-2, WL-8- 6, WL-8-7, WL-8-8, WL-8-9) were positive for Yr17 gene. All five PBW343-derived advanced lines confirmed for the Yr36/Gpc-B1 allele presence. Three advanced lines (PL-8-2, PL-8-3, PL-8-4) displayed the presence of the Yr18 gene. Two advanced lines (Pl-8-3, PL-8-5) confirmed presence of the Yr17 gene. All the advanced wheat lines derived from WH711 as well as from PBW343 displayed very high levels of resistance against yellow rust and better agronomic characteristics than the parents. Positive correlation was observed in yield and yield related traits. The hectoliter weights of PBW343-derived lines indicated their superiority in terms of good flour recovery than PBW343 and comparable to the checks. WH711- and PBW343- derived advanced wheat lines show better gluten quality and high quantity in sedimentation test. Gpc-B1 allele introgression provided high GPC in WH711 derived advanced wheat lines. Iron and zinc contents of both WH711-derived and PBW343-derived advanced wheat lines were at par when compared to either parent and checks, indicating successful bio fortification of both micronutrients. Two of the WH711-derived lines are being further tested in IPPSN/station trials.ThesisItem Open Access Development of an efficient protocol for micropropagation of strawberry (Fragaria ananassa Duch.)(CCSHAU, Hisar, 2020-07) Swati Rani; Upendra KumarStrawberry (Fragaria × ananassa Duch.) is the most important fruit worldwide. Micropropagation is mainly for the clonal multiplication.Strawberry cvs. Grenada and Petaluma were micropropagated for rapid shoot and root multiplication. Meristematic part and leaf primordia is source of explants. Foe the sterilization process, the explants were dipped in two to three drops of Tween 20 per 100 ml for 10 minutes with distilled water, 0.5% Bavisitin & Streptocycline for 30 mintues with antimicrobial supplement and 0.1% HgCL2 for 2 minutes gave the maximum aseptic cultures. After the surface sterilization of meristemetic part of plants 3-5 mm long was used as a explant. Multiplication stage results indicate that highest auxiliary buds were observed when MS medium supplemented with 1 mg/l and 1.5 mg/l BAP in Petaluma and Grenada. At rooting stage, it was clear visually that MS medium supplemented with 0.5 mg/l of BAP with 1.5 mg/l of IBA in both the cultivars gave the best results of enhanced number roots with higher length and number shoots with higher length per explants. The best result for root multiplication indicating the use of IBA with ( 1.0, 1.5 mg/l) concentration as compared to other treatments.The highest response for the shoot multiplication was obtained with MS containing 1.5 mg/l and 1.0 mg/l BAP respectively. The present research is very useful in commercializing the new cultivars of strawberry into north Indian conditions which gives higher yield of fruits.ThesisItem Open Access ―Phenotypic and molecular characterization of HD 2967 x Kharchia 65 F5 and backcross generations for salt tolerance in wheat (Triticum aestivum L. em. Thell”(CCSHAU, Hisar, 2019-08) Saini, Pooja; Yashveer, ShikhaSoil salinity is emerging environmental stress that reduces rate of germination, delays emergence and affects almost all stages of growth and development of wheat plants which ultimately leads to reduction in grain yield of crop.The present investigation focused on phenotypic and molecular characterization of selected wheat (Triticum aestivum L. em. Thell)genotypes of F5 and backcross (BC1F4, BC2F3, BC3F1) generations derived from HD 2967 X Kharicha 65 for salinity tolerance on the basis of presence of both Nax loci and their agronomic performance at salt stress level 8 dS/m. A total of 92 plants confirmed the presence of Nax1 locus at a band size of 210 bp consisting of 16 F5, 41 BC1F3, 9 BC2F2 and 27 BC3F1, a total of 53 plants confirmed the presence of Nax2 locus at a band size of 225 bp comprising of 6 F5, 35 BC1F4, 8 BC2F3 and 4 BC3F1.Overall, plants comprising of 6 F5,26 BC1F4, and 4 BC3F1 of HD 2967 x Kharchia 65were found to have both the Nax loci. On the basis of phenotypic and genotypic variations, 56 high yielding plants were selected for genotyping using polymorphic SSR markers. A total of 180 SSR primers were used to study the polymorphism between parents HD 2967 and Kharchia 65, out of which 27 SSR primers showed polymorphism. Polymorphism information content (PIC) value in the studied genotyped ranged from 0.191 (xgwm480) to 0.425 (wmc3) with an average value of 0.339. Cluster tree analysis using NTSYS-pc software, F5 and backcross (BC1F4, BC2F3, BC3F1) generations showed that all the selected plants were inclined toward recurrent parent HD 2967 representing higher similarity with HD 2967. From above analysis, high grain yielding and salt tolerant plants were selected such as plant number 259Q3c, 271Q3c (F5); 202P9b, 303P2b and 202P3b (BC1F4); 241R2c and 243R1a (BC2F3); 25(3)1b and 24(1)1c (BC3F1) and these plants could be further backcrossed with the parent HD 2967 to develop salt tolerant wheat lines.ThesisItem Open Access In silico identification and physical mapping of gene(s) responsible for β-glucan in Bread Wheat (Triticum aestivum. L)(CCSHAU, Hisar, 2020-07) Dhamija, Aryan; Upendra KumarDietary fibers from plant cell wall are an essential component of healthy foods. Higher intake of dietary fiber reduces the risk of diet related chronic disease like type 2 diabetes, obesity and also improves gastrointestinal health. In human diets, cereal fiber is the largest contributor to total dietary fiber consumption. The soluble dietary fiber (1-3) (1-4) mixed linked β-D-glucan from cereal grains is a valuable component of a healthy diet. In the present investigation, the gene responsible for β-glucan in hexploid wheat was identified using both in silico and molecular methods.The in silico analysis confirmed that CslF6 gene in wheat was located on the chromosome 7A. Sequence similarity search was conducted between HvCslF6 and wheat survey sequences displayed a similarity of 96% with chromosome 7A. The structure of this gene had 3 exons and codes for a protein of 945 amino acids. Homology modeling of putative CslF6 protein was described as Probable cellulose synthase A catalytic subunit 8.Different cytogenetic stocks of Chinese spring revealed that CslF6 gene was located on centromeric region of 7AL (FL=0.29). Identified CslF6 in wheat can be utilized to make β-glucan efficient wheat and can be used in biofortification program.ThesisItem Open Access Effect of Zinc sulphate on fruit drop, yield and quality of Kinnow mandarin(CCSHAU, Hisar, 2020-09-28) Ayoub, Mohammad; Rana, G.S.The study entitled “Effect of zinc sulphat on fruit drop, yield and quality of Kinnow mandarin” was conducted on nine years old ear marked plants at Experimental Orchard in Post-harvest Technology Laboratory of the Department of Horticulture, CCS Haryana Agricultural University, Hisar during the year 2019-20 to study the effect of Zinc sulphate on fruit drop, yield and quality of Kinnow mandarin. The experiment comprised of three levels soil application of Zinc sulphate (50,100,150 g per plant) and foliar application various concentration of Zinc sulphate( 0.5and 0.75%) and urea(1and1.5%). There experiment was conducted in randomized block design. The results of the experiment indicate that the foliar application of Zinc sulphate and Urea had a considerably affected most of the recorded parameters. Soil application of 150 g Zinc sulphate followed by the foliar spray of the 0.5 per cent of Zinc sulphate along with 1 per cent urea resulted into maximum fruit retention (53%), number of fruits per plant (533.87), size of fruits [fruit length (6.04 cm) and diameter (7.10 cm), average fruit weight (165 g), fruit yield per plant (83.87 kg), juice content (44.45%), total soluble solids (9.80%) and TSS to acid ratio (12.09 ) in Kinnow mandarin plants. The same treatment successfully resulted in minimum June (39.08%) and pre-harvest fruit drop (11.7%), acidity (0.81%), rag (31.89%) and peel content (22.69%) and peel thickness (2.83 mm).ThesisItem Open Access To evaluate the compatibility and efficacy of post-emergence herbicidal combinations tank mixed with zinc and iron sulphate against weeds in wheat (Triticum aestivum L.)(CCSHAU, Hisar, 2020-07) Jitender; AmarjeetWheat (Triticum aestivum L.) is one of the major cereal food crops of the world. Weeds are one of major biotic factors affecting wheat production significantly and now a days, micronutrients deficiency is also being observed in mostly wheat growing areas. Very less information is available regarding compatibility of herbicides with micronutrients. Field experiment was conducted at RRS, Bawal during Rabiseason of 2018-19 to evaluate the compatibility of post-emergence herbicidal combinations with Zn or/and Fe. Experiment consisted of 18 treatments replicated thrice, was laid outin Randomized Block Design. Four herbicidal combinations viz. clodinafop + metsulfuron@ 60 g/ha, sulfosulfuron + metsulfuron @ 32g/ha, mesosulfuron + iodosulfuron @ 14.4 g/ha, pinoxaden + carfentrazone @ (50 + 20g/ha) were evaluated for compatibility with Zn {ZnSO4(0.5%) + urea (2.5%)}, Fe {FeSO4 (0.5%)} and with both Zn as well as Fe {ZnSO4 (0.5 %) + urea (2.5 %) + FeSO4 (0.5 %)}. The results of experiment revealed that all four herbicidal combinations studied were found compatible with Zn and Fe as well. Tank mixing of herbicidal combinations with Zn or Fe exhibited a bit improvement in growth and yield of wheat. While, tank mixing of both Zn and Fe with herbicidal combinations showed significant increase in growth parameters and subsequently grain as well as biological yield of wheat as compared to sole application of respective herbicidal combination. Among all the treatments, application of mesosulfuron + iodosulfuron (14.4 g/ha) + ZnSO4 (0.5%) + urea (2.5%) + FeSO4 (0.5%) was observed as most productive with highest grain yield (5655 kg/ha) and profitable with highest net returns (Rs. 60973/ha) and B:C (1.88).Satisfactory weed control was observed with all the treatments of herbicidal combinations Tank mixing of Zn and Fe improved the efficacy of herbicidal combinations by improving competitive ability of wheat through better growth resulting into higher weed control efficiency. The uncontrolled weeds caused 34 per cent loss in grain yield.ThesisItem Open Access Performance evaluation of tractor drawn straw reaper for paddy straw(CCSHAU, Hisar, 2019-08) Ujala; Saroha, AnilRice (Oryza sativa) is one of the leading and widely cultivated cereal crops in the world. India has the world‟s largest area under rice with 43 million ha and is the second largest producer next only to China with production of 104 million tonnes during 2015-16. The productivity of rice was around 24q ha-1 during the agricultural year 2015-16 (Anonymous, 2017). The tractor drawn paddy straw reaper was evaluated at farmers field in Ludas village of Hisar district for optimization of machine parameters viz., cylinder speed (550, 750 and 950 rpm), forward speed (1.0, 1.5 and 2.0 km h-1) and crop parameter viz., straw moisture content (20.4 to 49.5 %) in relation to field capacity, field efficiency, fuel consumption, straw size, straw recovery and straw split percentage for paddy varieties Pusa-1121 and Pusa-44. For the paddy variety Pusa-1121 the field capacity, field efficiency, fuel consumption and power consumption at optimum conditions were 0.23 ha h-1, 66.37%, 5.33 l ha-1 and 21.82 kW, respectively. The straw size, straw recovery and straw split at optimum condition were 3.30 cm, 65.11 % and 89.11 %, respectively. Similarly for paddy variety Pusa-44, the field capacity, field efficiency, fuel consumption and power consumption at optimum conditions were 0.20 ha h-1, 59.45%, 5.67 l ha-1 and 23.70 kW, respectively. The straw size, straw recovery and straw split at optimum condition were 3.46 cm, 62.89 % and 86.23 %, respectively. Moisture content of 20.4-20.8 per cent, forward speed of 1.5 km h-1 and cylinder speed of 950 rpm was found optimum for paddy varieties Pusa-1121 and Pusa-44. The cost of operation was Rs 5190 ha-1and Rs 6112 ha-1 in Pusa-1121 and Pusa-44 variety, respectively. The machine was found economically feasible for the farmer‟s.ThesisItem Open Access Effect of nitrogen levels under different spacing on coriander (Coriandrum sativum L.)(CCSHAU, Hisar, 2020) Chaman; Malik, T.P.The present study entitled, “Effect of nitrogen levels under different spacing on coriander (Coriandrum sativum L.)” was conducted during the Rabi 2019-20 at research farm, Department of Vegetable Science, Chaudhary Charan Singh Haryana Agricultural University, Hisar. The nitrogen doses and different row to row spacing plays important role in growth, yield and quality of coriander crop. In the present investigation four nitrogen levels (0, 25, 50 and 75 kg ha-1) and three different row to row spacing (20 x 15 cm, 30 x 15 cm and 40 x 15 cm) were taken into consideration. The nitrogen was applied in two split doses i.e. 50 per cent at sowing and remaining dose at 40 days after sowing (DAS). Vegetative growth parameters i.e. days to emergence, plant height, primary branches and secondary branches (45, 60, 90 DAS and at harvest), yield and yield attributes were recorded. The quality parameters i.e. test weight, electrical conductivity, standard germination and vigour indices were also recorded in in-vitro condition after harvesting. It was observed that nitrogen dose at 75 kg ha- 1 and row to row spacing 40 x 15 cm was well suited for vegetative growth except plant height that was best in 20 x 15 cm spacing. The seed yield (2119 kg ha-1) and other yield attributes were found highest at 75 kg N ha-1 with spacing 40 x 15 cm and lowest in control. The quality parameters were recorded highest with 75 kg N ha-1 and spacing 40 x 15 cm and the highest cost of cultivation (Rs. 48445 ha-1) was occurred in coriander sown at higher nitrogen levels under different spacing. Among different treatments, highest gross returns of Rs. 158925 ha-1 and highest net returns of Rs. 110480 ha-1 were obtained at 75 kg N ha-1 with 40 x 15 cm. While comparing the, benefit cost ratios, highest B: C (2.29) was recorded at 50 kg N ha-1 with 30 x 15 cm.
