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  • ThesisItemOpen Access
    Assessment of Nutritional Status of Pregnant Women With Special Reference to Anaemia and Obesity in District Hisar ( Haryana)
    (CCSHAU, 2016) Sethi, Divya; Dahiya, Saroj
    The present study was conducted in Hisar district of Haryana state to assess the nutritional status of pregnant women and impart nutrition education to the selected pregnant women. A total of 250 subjects having pregnancy of third trimester selected randomly from different hospitals of district Hisar. Respondents from both govt. and private hospitals selected so as to draw a sample of both urban and rural women.Nutritional status of pregnant women was assessed in terms of dietary assessment, anthropometric measurement (height, weight and skin fold thickness) and clinical assessment of signs and symptoms of various nutrient deficiency diseases. Dietary assessment was done by 24 hour dietary recall method for three consecutive days of 100 respondents. The results of the study revealed that consumption of all food stuffs like cereals, pulses etc were lower in the diets of pregnant women. Nutrients like energy, fat, β- carotene, B-complex vitamins, Vitamin C, Iron and Calcium were found limiting in the diets of pregnant women. It was found that caste, family size, income, working status and education of respondents and husband‘s education had significant (P<0.05) effect on food intake of selected pregnant women. Effect of caste, family size,family type, income, respondent‘s occupation and education, husband‘s occupation and education were found to be significant (P<0.05) on nutrient intake of the pregnant women. Result indicated that out of 250 pregnant women 79.2 per cent pregnant women were anaemic while 20.8 per cent were non anaemic. Effect of type of family and income were significant (P < 0.05) on prevalence of anaemia. Majority 47.2% women were overweight, 25.2 percent obese, 22.8 percent normal weight and 4.8 percent underweight.Nutrition education was imparted to 50 selected pregnant women. It was found that majority of the responents had inadequate knowledge regarding nutrition before imparting nutrition education. After imparting nutritional education there was significant (P<0.01) improvement in knowledge of pregnant women in all the characteristics.
  • ThesisItemOpen Access
    Genetical, Biochemical and Molecular Basis of Cotton Leaf Curl Virus Disease in Gossypium hirsutum L.
    (CCSHAU, 2016) Sonika; Sangwan, R.S.
    The inheritance of cotton leaf curl virus disease indicated the complementary type of gene interaction (9:7) which indicated that this trait was digenically controlled. Generation mean analysis revealed significant differences for some traits where the A, B, C and D individual scales were also significant for some characters indicated thereby the presence of non-allelic interactions. Additivedominance model was found fit for days to flower in crosses (I, II and III), boll number in crosses (II, III and IV), boll weight in crosses (I, II and IV), GOT in all the four crosses, seed index in cross (IV), lint index in all the four crosses and seed cotton yield in crosses (I, III and IV). Dominance component was significant for most of the characters. Duplicate type of interaction was apparent for days to flower (cross IV) and plant height in crosses (I, II and IV). Among biochemical parameters, sugar content was significantly higher in susceptible parents than resistant parents. Phenol, tannin, gossypol content and enzymatic activities of peroxidase and polyphenoloxidases were significantly higher in resistant parents as compared to susceptible parents. Oil content did not show any particular trend. The correlation matrix among different biochemical parameters revealed that cotton leaf curl virus disease (CLCuD) grading (0-6) at 60 DAS, showed positive significant correlation with sugar content while other biochemical parameters viz. phenol, gossypol, tannin, crude protein and enzymes peroxidase (PO) and polyphenoloxidase (PPO) showed significant negative correlation. CLCuD grading did not show any correlation with oil content. The same trend was observed in all of the four crosses at 60 and 90 days after sowing (DAS) except in sugar, phenol, polyphenoloxidase (PPO) and protein (90 DAS). Twenty eight ISSR primers were used to study molecular variation among parental genotypes and their F1s. A total of 175 alleles were amplified unambiguously by 28 ISSR primers, of which 127 alleles were polymorphic (72.57 per cent). Polymorphic information content (PIC) value was found in the range of 0.495 to 0.907. The ISSR primer UBC 834 was found to have maximum PIC value (0.907) which indicated that this primer is more informative and can be further used to identify resistant genotypes from the germplasm or breeding material. The cluster analysis led to the distribution of parents and their F1s in to different groups at the similarity coefficient value of 0.54-0.85. Cluster A, included parent HS 6, cluster B bifurcated further into different sub-clusters indicated that parents GCH 3 and H 1353 and their F1s were present on the upper side of the dendrogram and parents HS 6 and RST 9 on the lower side of the dendrogram. Genetic similarity by ISSR analysis showed that parental genotypes GCH 3 and HS 6 were quite distinct from each other.
  • ThesisItemOpen Access
    Molecular mapping of bacterial blight resistance gene, drought tolerant QTL(s) and genetic diversity analysis in clusterbean {Cyamopsis tetragonoloba(L) Taub}
    (CCSHAU, 2011) Bajaj, Anshu; Boora, K.S.
    The present investigation was carried out to screen genetic diversity using RAPD markers and to identify and map the QTL for resistance to bacterial blight and drought tolerance in clusterbean .The plant material was procured from Forage section . For bacterial blight, the resistant (HG 75) & susceptible(PNB) genotypes were selected & crossed to generate F3 population. The F3 population was used to map QTLs. For drought tolerance studies genotypes HG 563 (tolerant) & PNB (susceptible) were used to generate the mapping population (F 2:3).The genetic diversity studies were carried out in 48 genotypes of clusterbean using 130 RAPD primers and 97 primers produced amplification of 705 alleles ;out of which 644 were polymorphic. The similarity ranged from 0.11 to 0.97.The dendrogram grouped the genotypes into two major clusters at a similarity coefficient of 0.68. A variety FS277 was out grouped which is a forage variety. The genotype GP96 and F5277 were found to be most diverse (89%).The QTLs were identified for bacterial blight resistance using parental genotypes (HG75 & PNB) & their F3 population. The F 3 population was screened artificially for disease resistance along with the parent genotypes. Polymorphic primers were used to screen the F3 population & the major QTLs were identified for disease resistance using parental genotypes, HG75, PNB and their F 3 population. A total of 83 RAPD primers were used to screen the parental genotypes out of which nine primers were polymorphic. These primers were used to screen the F 3 population and the QTLs found were L19 , D1, AB7 and QLTY 3. Parental genotypes HG563 and PNB and their F2-3 genotypes were screened at field and net house for resistance to drought. Out of fifty genotypes, 12 were highly tolerant, 23 were moderately tolerant and 15 were susceptible to drought. The parents were screened with eighty seven primers out of which, sixteen were found polymorphic and nine QTLs were found linked to drought tolerance. Loci OPQ 20,OPD10, OPD14,OPQ 12,OPAC 8 and OPF 9 were major contributing QTLs and OPAC2,OPL3 and OPF17 are minor QTLs The diverse lines may be used for breeding programs to improve the production of agronomically superior genotypes of guar. The major QTLs for bacterial blight and drought tolerance may be used for introgression into agronomically superior genotypes of guar.
  • ThesisItemOpen Access
    Comparative impact of insecticides on bacterial and acetylcholinesterase activity of Eisenia fetida (Savigny) and Pheretima posthuma (Kinberg)
    (CCSHAU, 2017) Yadav, Jyoti; Dharambir Singh
    The present study was carried out to analyze the toxicity of chlorpyrifos and carbofuran on E. fetida and P. posthuma. Paper contact toxicity test was used to determine LC50. LC50 of chlorpyrifos against adult E. fetida and P. posthuma was 0.25% and 0.11%, respectively. While LC50 of carbofuran against adult E. fetida and P. posthuma was 5.14% and 4.11%, respectively that confirms the greater sensitivity of P. posthuma towards insecticide induced toxicity in comparison to E. fetida. Lower LC50 value of chlorpyrifos confirms its higher toxicity as compared with carbofuran. Total 52 bacterial isolates were isolated from the gut of worms, out of which six resistant gut bacterial species having agricultural importance were identified as Sphingobacterium sp., Citrobacter freundii, Citrobacter murliniae, Bacillus australimaris and Burkholderia paludis. Inhibition in AChE activity due to chlorpyrifos and carbofuran exposure was observed at 24h. While E. fetida exposed to carbofuran tried to maintain AChE level, which may be seen as strategy to cope up with toxicity after prolonged exposure.
  • ThesisItemOpen Access
    Effect of nitrogen levels on wheat varieties under agri-silvi-horticultural system
    (CCSHAU, 2017) Johar, Vishal; Dhillon, R.S.
    The present investigation entitled “Effect of nitrogen levels on wheat varieties under agri-silvihorticultural system” was carried out in research area of Forestry Department, CCS Haryana Agricultural University, Hisar during 2015-16 and 2016-17 to study the effect of nutrient levels on yield attributes, yield and quality parameters of different wheat varieties (WH-1105, HD-2967, WH-711 and DPW-621- 50) in already established five year old agri-horti and agri-silvi-horti systems. The growth of eucalyptus and kinnow under both agri-silvi-horti and agri-horti system increased sharply with the advancement of age. Eucalyptus attained maximum (7.2 cm) CAI for basal diameter followed by DBH (5.1 cm) during the age of six year which shows that the eucalyptus achieved sufficient biomass during 6th year of growth. The additional dose of nitrogen (10, 20 and 30 per cent) to the recommended dose of fertilizer (RDF) in both the agroforestry based systems increased the growth, yield attributes and yield of different wheat varieties however; the difference between further additional doses of N to the RDF was nonsignificant. Among different wheat varieties, HD-2967 was found the most shade tolerant and exhibited higher plant height, number of tillers, number of spikes, spike length, number of grains per spike, grain and straw yield etc under both the agroforestry based systems. Whereas, the variety WH-711 was found be the most sensitive to shade and exhibited poor growth, yield attributes traits and yield. However, grain, straw and biological yield of different wheat varieties was found higher in agri-horti system over agri-silvi-horti system due to more competition among the annuals and perennials in agri-silvi-horti system. Different quality parameters viz. protein content, gluten content, sedimentation value, grain hardness etc. in different wheat varieties was observed higher in agri-horti and agri-silvi-horti system over sole cropping system. Higher protein content, grain hardness and grain luster were recorded in HD- 2967 while, variety WH-711 exhibited higher gluten content and sedimentation values under both the agroforestry based systems. Soil EC and pH decreased considerably under both the agroforestry based systems. However, soil organic carbon, available nitrogen, phosphorus and potassium increased significantly over the control (sole crop). After six year of experimentation the higher B:C ratio was recorded in agri-silvi-horti (2.06) followed by agri-horti (1.66) and sole cropping (1.39). Hence, agrisilvi- horti and agri-horti systems are economically viable, improve the soil health and provide nutrient security. Therefore, agroforestry based systems are eco-friendly and a way to increase the farmer’s income.
  • ThesisItemOpen Access
    Livelihood security of small and marginal farm families in rohtak division of haryana state
    (CCSHAU, 2017) Punia, Anusha; Kaushik, Sushma
    The present study was conducted in Rohtak division of Haryana state. Two districts viz. Sonipat and Karnal and one block from each district were selected randomly. Seventy five small and marginal farm families from each selected village were drawn randomly making total 300 respondents. Sixteen independent variables and three dependent variables were selected. Data was collected with the help of well-structured interview schedule. The inferences were drawn on the basis of frequency, percentage, weighted mean score and coefficient of correlation. The study revealed that majority of respondents belonged to 35-50 years of age, educated upto secondary, general caste, married, having nuclear family with medium family size, had farming as their main occupation. Majority of the respondents had negligible social participation, used high localite sources, medium cosmopolite and medium mass media source of information utilization. Majority of farm families used double cropping patterrn, had medium cropping intensity. Physical capital in both the districts was found high.Human capital, social capital and financial capitals were found of medium level while natural capital was low in Karnal district and medium in Sonipat district. Overall livelihood security index showedfood security was reported to be high while nutrition security, economic security, shelter/water and sanitation security, health security, education security and access to institution were found to be of medium level. Farm families faced lack of capital, high labour cost as their main livelihood problems. Video film on ‘Swachh Jal Behtar Kal’ was developed by using standard procedures. The effectiveness of Video Compact Disc (VCD) was assessed through several parameters. Effectiveness of Video Compact Disc (VCD) in terms of audio quality, visual quality, content importance and presentation of message was rated as high.Family size, family education status, occupation, annual income and information source utilization were positively and significantly correlated with all livelihood capitals except natural capital. Age, family size, family education status, occupation, annual income and information source utilization were positively and significantly correlated with all livelihood securities.
  • ThesisItemOpen Access
    Studies on the biology of onionwead (Asphodelus Tenuifolius cav.) and its herbicidal control in chickpea
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Sahai, Bhagwan; Khanna, S. S
  • ThesisItemOpen Access
    Assessing phosphate requirements based on intensity/capacity relationship in Haryana soils
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Yadav, Hanuman Dass; Karwasra, S.P.S.
  • ThesisItemOpen Access
    Studies on the incidence and control of Sarcoptic ange in Buffaloes with some systemic Insecticides
    (College of Veterinary Sciences Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Tikaram, Samuel Manly; Ruprah, N. S