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2020 - 20235
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Author: Pooja × End date: 2023 × Start date: 2020 × Thesis Type: M.Sc ×
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Now showing 1 - 5 of 5
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    ThesisItemOpen Access
    Computational studies of cytochrome P450 (CYPs) enzyme in pulses
    (CCSHAU, Hisar, 2023-10) Pooja; Ahalawat, Navjeet
    Cytochrome P450 (CYP450) is a large superfamily of enzymes found in all domains of life, including plants. It plays a crucial role in the metabolism of various compounds, including drugs, toxins, and endogenous substrates. Pulses are the richest source of fiber and complex carbohydrates, resulting in a low GI food. CYP450s enzymes are heme-containing monooxygenases that catalyze metabolisms of various endogenous and exogenous compounds. They constitute a superfamily of enzymes present in various organisms including mammals, plants, bacteria, and insects. CYP450s are diverse and metabolize a wide variety of substrates, but their structures are largely conserved. In this study, bioinformatics analyses of CYP450s enzyme performed in pulses. According to the conserved motifs obtained by MEME and MAST tools, ten motifs were common in all those pulses. The structural and functional analyses of CYP450s in pulses were done by using UniProtKB and InterProScan tools in ExPASy database. The tertiary structures of pulses were predicted by Phyre2 web server. The protein sequences were aligned with ClustalW and then the phylogenetic tree was constructed by MEGA11 using the neighbor-joining (NJ) method. By the successful analysis of CYP450s, we developed a productive database by using MYSQL and PHP. The database provides information related to 200 proteins of P450s in pulses with their predicted 3D structure in PDB format, functions and Fasta sequences. A Pulses Cytochrome P450 database serves as a centralized repository for curated information on CYP450 enzymes in pulses. It consolidates data from various sources, including sequence information, structural data, substrate specificity, and catalytic properties.
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    ThesisItemOpen Access
    Fabrication of bismuth telluride thin film and study of its electrical properties
    (CCSHAU, Hisar, 2023-05) Pooja; Paul Singh
    In this study, thin films of Bismuth Telluride were fabricated successfully by using thermal evaporation method onto different substrates (i.e., glass and silica) at ambient temperature. Their structural, morphological, optical and electrical properties were investigated by using X-ray diffraction (XRD), Field Emission-Scanning Electron Microscopy (FE-SEM), Photoluminescence (PL) sepectrooscopy and SourceMeter Instrument, respectively. The FT-IR study showed that the prepared Bismuth Telluride films do not show any transmittance spectra. The XRD analysis revealed that the films were crystalline in nature. The surface mimages showed that the prepared films have a homogeneous and compact grain surface. The calculated optical band gap was about 2 eV for both types of film. The I-V characteristics of prepared thin films were analyzed at temperature ranges from 30°C to 100°C. It was found that the films fabricated onto silica substrates showed larger electrical conductivity than that of the films fabricated onto glass substrates. Also, the increment in electrical conductivity was observed with the temperature showing that the prepared films have negative temperature coefficient of resistance and exhibit the semiconductor behaviour.
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    ThesisItemOpen Access
    Influence of manure and nano urea on productivity and quality of wheat (Triticum aestivum L.)
    (CCSHAU, Hisar, 2023-08-01) Pooja; Thakral, S.K.
    The present investigation entitled “Influence of manure and nano urea on productivity and quality of wheat (Triticum aestivum L.)” was carried out during Rabi 2022-23 at the Agronomy Research Farm, Department of Agronomy, CCS HAU, Hisar with the objectives to evaluate the effect of organic manures and nano urea on growth, quality, yield and economics of wheat. The research was carried out in factorial randomized block design with nine treatments with four replications. Factor A consisted of two wheat varieties (WH 1270 and DBW 303), assisted in main plot and while factor B comprised nine fertility levels, viz., 100% RDF, 125% RDF, 3 sprays of nano urea @ 500 ml/acre at 20-25, 40-45 and 60-65 DAS, 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS, 75% RDF + 2 sprays of nano urea @ 500 ml/acre at 20-25 and 40-45 DAS + PSB, 50% RDF + VC @ 4 t/ha + FYM @ 5t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS, 50% RDF + VC @ 4 t/ha + 2 sprays of nano urea @ 500ml/ acre at 20-25 and 40-45 DAS, 50% RDF + FYM @ 10 t/ha + Azotobacter @ 500 g/ha, 50% RDF + 2 sprays of nano urea @ 500 ml/acre at 20-25 and 40-45 DAS + Azotobacter @ 500g/ha + PSB in sub plots. Based on the present study it was be concluded that the variety WH 1270 recorded in significantly higher growth parameters such as plant height, dry matter accumulation, crop growth rate (CGR), relative growth rate (RGR) and yield attributing characters, viz., number of spikes per mrl-1 and 1000 grain weight as compared to the variety DBW 303. Higher grain yield (5154 kg ha-1) and straw yield (6099 kg ha-1) were registered by the variety WH 1270. Among the fertility levels, maximum plant height, crop growth rate, relative growth rate along with yield attributing like number of spikes per mrl-1, number of grains per spike and 1000- grain weight were noted by the treatment with application of 125% dose of fertilizer. This treatment resulted in dry matter accumulation, number of spikes per mrl-1,number of grains per spike and 1000- grain weight at par with the treatment with 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS. Maximum grains yield (5426 kg ha-1) was recorded with the treatment 125% RDF which was at par with the treatments 100% RDF (5350 kg ha-1) and 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS (5396 kg ha-1). Maximum straw yield (5422 kg ha-1) was observed with the treatment 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS were applied. Highest protein content (11.26%) was recorded with the treatment where 125% RDF was applied. It was 1.3% and 1.5% higher than the treatments with 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS (11.50) and 100% RDF (11.48). Highest net returns (₹ 86937 ha-1) and B: C (1.24) ratio were recorded with the treatment where 75% RDF + FYM at @ 5 t/ha with 1 spray of nano urea at 40-45 DAS was applied.
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    ThesisItemOpen Access
    Chemical profiling and biological evaluation of Vetiver (Vetiveria zizanioides N.) essential oil
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-08) Pooja; Ravi Kumar
    The present study was undertaken for chemical profiling and evaluation of Vetiver (Vetiveria zizanioides N.) essential oil of promising Sugandha variety from different samples of roots (sample 1, sample 2, sample 3 and sample 4). Commercially available VEO was obtained from St. Botanica pharmacy store, Panchkula, Chandigarh which was ordered online. Sample 1, sample 2, sample 3 and sample 4 were harvested after 10 months, months, 18 months and 5 years of sowing. The EOs used in the study, appeared as pale yellow viscous volatile liquids, obtained in 0.55, 0.50, 1.16 and 0.26 % yield from sample 1, sample 2, sample 3 and sample 4, respectively. The yield was highest in the roots harvested after 12 months of sowing and lowest in roots which was harvested very late approximately after 5 years. A significant variation was observed in chemical composition of these VEOs. VO3 had more diversity and VO1 had lesser diversity. However, the chemical composition of VO5 was totally different from the other studied oils. The antimicrobial activity of VEOs was studied against various bacterial and fungal strains. VO3 showed higher antibacterial activity against K. aeruginosa, X. axonopodis pv. cyamopsidis, S. aureus and VO1 showed higher antifungal activity against F. oxysporum and M. grisea. Whereas, VO5 displayed least activity against tested strains.
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    ThesisItemOpen Access
    Impact of Happy Seeder on Socio-economic status of farmers in Haryana
    (CCSHAU,HiSAR, 2020-08) Pooja; Tyagi, Rashmi
    Wheat is major cropping system in North West India with cropping intensity of nearly 200 %. The huge quantity of paddy straw after the combine harvesting of paddy is to be managed in 15-20 days to plant next succeeding wheat crop. Keeping in mind this short window of time, farmers prefer to burn rice residue after combine harvesting of crop because residues interfere with tillage and seeding operations for the next crop. According to a report by the Ministry of Agriculture & Farmers welfare (2018), total paddy straw produced in Haryana and Punjab together is 28.10 million tonnes (2018 estimates) out of which 11.3 million tonnes was burnt in the fields.. Though a number of machines are available for straw management, Happy Seeder is one of the unique techniques which is used for sowing wheat in standing stubbles of crop residue. The study was conducted in two agro-climate zones of Haryana namely dry zone and wet zone. Two districts i.e. Kaithal and Fatehabad were randomly selected from both the zones. From each of two selected districts, one block was i.e. Pundri from Kaithal & Ratia from Fatehabad were randomly selected for the purpose of the study. After the selection of blocks, a cluster of villages were randomly selected for the purpose of the study. 30 adopters and 30 non-adopters respondents were selected from each block on the whole 120 respondents were selected for the purpose of the study. The findings of the study revealed that Knowledge level of adopters was more as compare to non -adopters. From the discussion and data it was also found out that the major reasons for adoption were that happy seeder is a environment friendly technology, reduce fuel and labor cost, improves soil health & increase yield than conventional method whereas shortage of information, more hiring charges, more complex technology, not beneficial and not affordable were the reasons for non-adoption. Regarding socio-economic impact expenditure on social ceremonies increased after adoption of Happy Seeder such as expenditure on marriage, expenditure on children education. To increase the rate of adoption of Happy Seeder technology the extension activities should be increase among farmers.