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2013 - 20192
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Author: Anu × End date: 2019 × Start date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    Phenotypic and molecular analysis for morpho-physiological traits and yellow rust in wheat progenies derived from the cross between WH711/WH542
    (CCSHAU, Hisar, 2019-12) Anu; Vikram Singh
    The present investigation entitled “Phenotypic and molecular analysis for morpho-physiological traits and yellow rust in wheat progenies derived from the cross between WH711 / WH542” The experiments were conducted in the Department of Genetics and Plant Breeding, CCS Haryana Agricultural University (CCSHAU), Hisar, India during two successive years of Rabi seasons 2014-15 and 2015-16 with objective to determine variability, correlation path analysis and genetic divergence. Characterization of 238 progenies along with parent was done on the basis of 12 morpho-physiological traits which exhibited sufficient variation in all the traits. The differences between GCV and PCV values were less which showed little influence of environment. The correlation results revealed that plant height, number of tillers per plant, spike length, number of grains per spike, grain length, 1000 grain weight, biological yield per plant and harvest index expressed significant positive correlation with grain yield per plant across the generations and can be used for improving grain yield per plant. Path analysis of both generations revealed that biological yield per plant and harvest index had high direct effect on grain yield per plant while traits viz., number of tillers per plant, plant height and spike length contributed to grain yield per plant through biological yield per plant and harvest index. Based on D2 values, the progenies were grouped into 7 clusters in F3 and 8 clusters in F4 generation. The progenies included in clusters III, V and VI in F3 and V, VII and VIII in F4 generation were identified as divergent as well as having higher mean value for important yield component traits. The traits viz., grain breadth, number of grains per spike, spike weight, number of tillers per plant and 1000 grain weight contributed more than 60% towards total variability. Out of 438 SSR primers pairs (including Yr specific primers) used to characterize the parents for Yr gene and agronomic traits. Among 217 SSRs gave amplification. Out of these 217, SSR 145 markers were monomorphic and 72 SSR markers (including twelve Yr specific markers) were found polymorphic on parents. It was confirmed by NTSYS-PC UPGMA cluster tree and dendrogram that two parents were most genetically diverse and the progeny lines were interspersed between the two parents for yellow rust in both the generations. Single marker analysis indicated that markers Xgwm249 and Xgwm297 were linked with yellow rust resistance in F4 generation.
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    ThesisItemOpen Access
    Assessment of nutritional status and impact of nutrition education on renal calculi patients
    (CCSHAU, 2013) Anu; Boora, Pinky
    The present study entitled “Assessment of Nutritional Status and Impact of Nutrition Education on Renal Calculi Patients” was undertaken to study the etiological factors, dietary habits, nutrients intake of the males and females respondents (control group and renal calculi patients) and to study the impact of nutrition education on dietary assessment and biochemical profile of selected renal calculi patients (males and females) from Ambala and Hisar city of Haryana state. On the basis of survey of 400 respondents (200 control group and 200 renal calculi patients) it was found that majority of the respondents were in the age group of 30 to 40 years, belonged to general category, living in nuclear family, engaged in service and monthly income was `25, 001 to 50,000. Majority of the respondents suffered from urinary tract infections. Majority of the renal calculi patients consumed more than 4 cups of tea /day and drink less than 4 glasses of water/day. Majority of the respondents (control group and renal calculi patients) were vegetarian in both the cities. Mean daily intake of pulses, green leafy vegetables, fruits, milk and milk products, fats and oils and sugar and jaggery by renal calculi patients (male and female) were found higher than RDA. Majority of the respondents of control group consumed marginally adequate amount of pulses, green leafy vegetables, roots and tubers and fruits in their diet, whereas majority of renal calculi patients consumed adequate amount of cereal, green leafy vegetables and milk and milk products in their diet in both the cities. Majority of the renal calculi patients consumed adequate amount of energy, fat, calcium, iron, riboflavin, vitamin C and β- Carotene, whereas majority of the respondents of control group consumed marginally adequate amount of protein, fat, calcium, phosphorus, thiamine in both the cities. Out of 200 renal calculi patients, 120 renal calculi patients (60 renal calculi patients from each city) were selected. Nutrition education was imparted to renal calculi patients with the help of audio- visual aids. At the end of 3 months of imparting nutrition education, the impact was assessed in term of their dietary intake, water intake, biochemical profile and gain in knowledge. Before imparting nutrition education, majority of the renal calculi patients consumed adequate intake of green leafy vegetables, milk and milk products and pulses, while after imparting nutrition education majority of the renal calculi patients (male and female) consumed marginally adequate amount of green leafy vegetable, milk and milk products and pulses in Ambala and Hisar city. After imparting nutrition education majority of the renal calculi patients consumed 7 to 8 glasses of water/day. The significant reduction (P<0.05) was observed in calcium, uric acid and creatinine in blood and urine samples of renal calculi patients of both the cities. Majority of the renal calculi patients had positive for pus cell, RBC and crystal (calcium oxalate).Gain in knowledge scores was significantly (P<0.01) higher after imparting nutrition education by renal calculi patients in both the cities. Hence, it can be inferred from the present study that nutrition education can be effective measure for bringing the significant changes in the nutritional profile as well as nutrition knowledge of renal calculi patients.