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ThesisItem Open Access Determining the relative efficacy of vermicompost, FYM and chemical fertilizers on crop yield of some vegetable crops(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Kumar, Dinesh; Sihag, R. CThesisItem Open Access Studies on seasonal gonadal changes 1n common house rat, Rattus rattus Linnaeus(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Vir1nani, Shelly; Pasahan, S. CThesisItem Open Access Studies on the activities of insect-pollinators of cucurbit crops(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Kumar, Surender; Gahlawat, S. KThesisItem Open Access Studies on the nematophagy in acarine fauna and their feeding potential on seed gall nematode(College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Rani, Ranjana; Gulati, RachnaThesisItem Open Access Studies on Pesticide lnduced Changes in Soil Acarine Fauna and the Nutrients in Poplar (Populus sp.) Plantation(College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Neha; Jain, K.L.ThesisItem Open Access Survey, isolation and identification of bacterial diseases of fresh water fishes in district Hisar, Haryana(CCSHAU, 2005) Dahiya, Tejpal; Sihag, R.C.;The present investigation was carried out to identify fish pathogens causing fish diseases in catla, rohu, mrigal, common carp, puthi and magur. A number of biochemical tests were carried out for identification of bacterial fish pathogens. The bacterial diseases were identified viz. epizootic ulcerative syndrome, fin and tail rot, hemorrhagic septicemia and vibriosis in three selected fish farms (Mirka, Satrod and SLR, Hansi) from July 2004 to March 2005. These diseases were reported in July, August, October and November. No disease was found in September, December, January, Febuary and March. Ten gram negative ( Aeromonas hydrophila, Enterobacter cloacae, E. gergoviae, Pseudomonas sp., P. pseudomallie, Klebsiella pneumoniae subsp. aerogenes, Providencia sp., Serratia marcescens, Vibrio anguillarum, V. alginolyticus) and two gram positive (Micrococcus roseus, Streptococcus gp Q1) bacterial pathogens were identified from infected fishes. In EUS, fungal hyphae were found penetrating deep into muscles in catla. In fish farm of Satrod, vibriosis was reported in magur. The bacterial pathogens (Vibrio anguillarum, V. alginolyticus) were isolated from the skin lesions. These bacteria are generally found in marine, brackish and rarely in fresh water. Deviation of optimal range of hydrobiological parameters led to infection in fishes resulting in to disease outbreaks.ThesisItem Open Access Molecular characterization of bacterial pathogens of fresh water fishes(CCSHAU, 2006) Ravikant; Gahlawat, S.K.The present investigation was carried out to identify the fish pathogens causing diseases in (catla, rohu, mrigal, grass carp, puthi, murrels and Thai magur). A number of biochemical tests were carried out for identification of bacterial fish pathogens. The bacterial diseases were identified viz. EUS, fin and tail rot, gill rot and haemorrhagic septicemia in village ponds/ fish farms (Charanjeet fish farm, Hansi, Siswala village pond, Satrod village pond, Ishwar Singh fish farm, Dabra, Rajli village pond, Kirtan village pond) from July, 2005 to March, 2006. Diseases were observed in July, September, November, 2005 and January, 2006. No disease was found in August, October, December, 2005 and January, 2006. Two gram positive cocci (Staphylococcus aureus, Streptococcus grp.Q1) and seven gram negative rods (Aeromonas hydrophila, Enterobacter aerogenes, E. cloacae, Klebsiella oxytoca, Citrobacter koseri, Pseudomonas aeruginosa, P. fluorescens) bacterial pathogens were isolated and identified from affected fishes. Amplification of genomic DNA of seven isolates (S. aureus, Kl. Oxytoca, E. cloacae, A. hydrophila, P. fluroescens, P. aeruginosa, Streptococcus grp Q 1) was carried out and ARDRA profiles were prepared by using Hinf1 and SauIIIA restriction endonucleases, separately. Phylogenetic similarity of all seven isolates was analyzed by NTSYSpc 2.0 programme. On the basis of similarity coefficient the isolates were divided in five major groups (A, B, C, D, and E). Group A is most diverse, which had genotype S. aureus followed by group E and group C contained genotypes Streptococcus grp. Q1 and E. cloacae, respectively. The genotypes Kl. oxytoca and A. hydrophila had 83% similarity, in-group B whereas P. fluorescens and P. aeruginosa had 99% similarity in-group D.ThesisItem Open Access Isolation and characterization of disease causing agents in freshwater prawn (Macrobrachium rosenbergii)(CCSHAU, 2006) Jakhar, Vijayanti; Gahlawat, S.K.A variety of diseases have been observed in larval, juvenile and adult freshwater prawns viz. as tail rot, larval mid cycle, bacterial necrosis, black spot disease, body cramp, black gills, and vibriosis in three selected fish farms (Ladwa, Mirka farm, Hisar and Charanjeet farm, Hansi) from July to December,2005. Tail rot disease was found in all the months during the course of work. The emergence of disease is actually the result of the interaction between abiotic and biotic factors. Keeping in view the above facts, the present investigations were carried out to identify disease causing pathogens in freshwater prawn, M. rosenbergii. A number of biochemical tests were performed on sugars, alcohol and amino acid etc were carried out for the identification of bacterial pathogens. Eighteen gram - negative rods (Aeromonas hydrophila, Enterobacter aerogenes, Escherichia coli, Klebsiella oxytoca, Kl. pneumoniae subsp. aerogenes, Kl. pneumoniae subsp. ozaenae, Citrobacter freundii, C. koseri, Pseudomonas aeruginosa, P. alcaligenes, P. putida, P. stutzeri ,P. fluorescens,Vibrio alginolyticus, V. splendidus, V. logei, V. parahaemolyticus, V. diazotrophicus, V. fluvialis) three gram positive - bacilli (Lactobacillus casei, L. brevis, L. lactis) and five gram - positive cocci (Micrococcus luteus1, M. varians, Cellobiosococcus sciuri, Staphylococcus aureus, Streptococcus grp Q1) were found associated with these diseases. Fluctuations in optimum range of physicochemical parameters provide optimum environment for the growth of bacterial pathogens which resulted the prawns in stress conditions and hence, causes diseases.