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2019 - 201912020 - 20231
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Subject: Vegetable Science × Author: Tejinder Singh × Start date: 2003 ×
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    ThesisItemOpen Access
    Molecular diversity, combining ability and heterosis studies in tomato (Lycopersicon esculentum Mill.)
    (CCSHAU, Hisar, 2023-08) Tejinder Singh; Bora, Lila
    The experiment was conducted at Regional Research Station, Uchani, Karnal, CCS Haryana Agricultural University, Hisar during Rabi 2021 and Rabi 2022. The analysis of variance for the conducted study revealed highly significant differences among lines, testers and crosses for all morpho-phenological, yield, and fruit quality characters studied, revealing the presence of highly significant variability in these tomato genotypes. GCV were slightly lower than respective PCV for almost all the characters under study, indicating that these were primarily consequence of genotypic differences. High heritability coupled with high genetic advance was found for all the traits except then the days to last picking, equatorial diameter, acidity, ascorbic acid and specific gravity etc. GCV as well as PCV was found high for the traits plant height, number of branches per plant, weight of marketable fruits per plant, weight of unmarketable fruits per plant, yield per plant, yield per hectare and number of locules per plant. The genotype K. Aman in lines and genotype K. Hemant in tester were found to be the best general combiner for yield and its attributing traits while for the quality traits A. Vikas in lines and Sel-7 in tester were found to be the best general combiners. Therefore, these lines and testers might be used as promising parents for hybridization programmes. The maximum average heterosis for the yield per hectare was showed by the F1 genotype K. Aman x Sel-7 followed by K. Aman x Sel-7 and K. Aman x K. Hemant. Maximum heterobeltiosis for the yield was showed by the genotype K. Aman x K. Hemant followed by the K. Aman x PHS. Out of 30 cross combinations, 21 crosses showed the positive economic heterosis over national standard check A. Apeksha, maximum economic heterosis was showed by the K. Aman x K. Hemant followed by K. Aman x PHS and P. Gourav x K. Hemant. The crosses viz., K. Aman x PHS, K.Aman x K. Hemant showed desirable heterosis for weight of marketable fruits per plant (g), weight of unmarketable fruits per plant (g), yield per plant and yield per hectare (q) etc. The yield per plant (g) and yield per hectare (q) were found to be significantly positively correlated with days to 50% flowering, days to first picking, days to last picking, weight of unmarketable fruits per plant and weight of marketable fruits per plant. The trait total soluble solids is significantly negatively correlated with plant height, days to 50% flowering, days to last picking, weight of marketable fruits per plant, weight of unmarketable fruit per plant and early fruit yield per plant. All the 10 primers were found polymorphic and amplified overall 30 alleles in which 22 alleles were polymorphic displaying clear bands size differences.
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    ThesisItemOpen Access
    In vitro propagation and genetic fidelity testing in potato (Solanum tuberosum L.)
    (CCSHAU, 2019) Tejinder Singh; Panghal, V.P.S.
    The present experimental study was conducted to standardize an efficient protocol for rapid multiplication of two important cultivars (Kufri Pushkar and Kufri Ganga) and to check genetic fidelity using molecular markers. The findings showed that the surface sterilization with 0.2% Bavistin + 0.4% streptocyclin for 45 minutesfollowed by 0.1% HgCl2 treatment for 60 seconds was optimum for in vitro culture establishment and maximum survival percentage. In cv. Kufri Pushkar,the minimum number of days (3.3) required for bud initiation and highest number of buds/explant (3.6) were obtained on MS medium fortified with 0.25 mg/l BAP + 0.5 mg/l KIN, while, in cv. Kufri Ganga, 3.2 days were required for bud initiation and 3.4 buds were obtained on the same medium. Maximum number of shoots/explant (4.4) were recorded when auxins were used in combination with cytokinins at a concentration of 0.01 mg/l IAA + 0.25 mg/l BAP in cv. Kufri Pushkarand 4.7 shoots per explant were observed in cv. Kufri Ganga. Number of days required for root initiation (2.3) was recorded to be minimum on rooting medium consisting of MS medium+2.5 mg/l NAA and MS medium + 1.5 mg/l NAA in cv. KufriPushkarand 2.2 days in cv. Kufri Ganga. Maximum number of roots/explant (14.0) were observed on MS medium fortified with 2.0 mg/l IBA in cv. Kufri Pushkarwhile 14.2 number of roots were recorded in cv. Kufri Ganga on 21th day of subculturing. Maximum 100 % rooting was observed in all MS media supplemented with different concentrations of auxins. The highest survival per cent (100%) of in vitro raised plants was recorded in potting media containing cocopeat, perlite and vermiculite in 3:1:1 ratio in both the cultivars. In vitro raised plants were assessed for genetic fidelity by using twenty RAPD primers. Out of twenty primers screened, four primers produced amplification while sixteen primers did not show any results in both the cultivars Kufri Pushkar and Kufri Ganga.DNA banding pattern of all tissue culture raised plants and mother plant was found similar and monomorphic explaining that all the plants raised through tissue culture using shoot tips were true to type and genetically identical to the mother plant.