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2019 - 201912020 - 20231
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Subject: Seed Science and Technology × Author: Nisha × Type: Thesis ×
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    ThesisItemOpen Access
    Seed quality assessment in natural and artificial aged seed of tomato and eggplant
    (CCSHAU, Hisar, 2023-01) Nisha; Bhuker, Axay
    The seed of two important solanaceous crops tomato (Solanum lycopersicum L.) and eggplant (Solanum melongena L.) was procured from Department of Vegetable Science and study was conducted to assess the effect of natural and artificial ageing on seed quality parameters and their correlation and to find out the effect of priming and storage containers on seed quality at Department of Seed Science and Technology, CCS HAU Hisar during 2020-2022. Under natural and artificial ageing storage period most of the seed quality parameters showed decreasing trend as the storage period increased in both cloth and polythene bag. Cloth bag maintained the germination upto 12 months while polythene bag maintained germination up to 15 months above Indian Minimum Seed Certification Standards (>70%). The polythene bag maintained 60.67 & 64.00% germination while in cloth bag it was 55.67 & 60.00% after 18 months of storage under ambient conditions in tomato and eggplant seeds respectively. The other seed quality parameters i.e. seedling length, seedling dry weight, vigour indices, viability percentage, speed of emergence index, seedling establishment, DHA, SOD, CAT, POD were found superior in polythene bag as compare to cloth bag. The mean emergence time and fungus infection was observed less in polythene as compare to cloth bag.The germination percentage after 24 h (72.67, 74.00%) of artificial ageing were found at par with the values of germination after 12 months (73.67, 77.00%) of natural ageing in polythene bag for tomato and eggplant respectively. Seeds primed with GA3 @ 50 ppm can enhance 24.6 percent germination in tomato and 21.2% in eggplant over unprimed seed in 18 months old seed. GA3 @50 ppm is also effective in controlling fungal infection and enhancement of other seed quality parameters i.e. seedling length, seedling dry weight, seed vigour indices, viability percentage, catalase activity, peroxidase activity, dehydrogenase activity, superoxide dismutase activity, speed of emergence index and seedling establishment. It is concluded from the study that polythene bag is a suitable container and GA3@50ppm is suitable priming agent for tomato and eggplant seeds. Germination percentage after 12 months of natural storage can be estimated through accelerated ageing test for 24 hours in tomato and eggplant crop.
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    ThesisItemOpen Access
    Seed quality assessment in naturally aged seed of sorghum [Sorghum bicolor (L.) Moench]
    (CCSHAU, 2019) Nisha; Jakhar, S.S.
    Two sorghum variety produced during kharif seasons were evaluated for their performance for different viability and vigour parameters viz. test weight, seed density, standard germination, seedling length, seedling dry weight, vigour Index-I, vigour Index-II, tetrazolium test, electrical conductivity, dehydrogenase activity test, seed mycoflora, speed of emergence index, mean emergence time and seedling establishment (%). Significant amount of variation was observed in both of the varieties and all the seed lots for all characters. In natural aged seed lots, only HJ541 variety of sorghum sustained their germination up to one year showed that sorghum seed can be stored at ambient condition up to one year without loosening its viability and after that the germination falls below standard. HJ541 was found superior variety based on majority of the viability and vigour parameters results whereas HC136 was found inferior. During natural ageing, standard germination, seed density, test weight, seedling length, vigour indices, viability, speed of emergence index and seedling establishment decreased significantly and progressively with the ageing period. The activity of DHA decrease significantly with storage period in both the varieties. Electrical conductivity was found significantly & negatively correlated with standard germination, indicating that the more the EC value lower will be the germination in laboratory as well as in the field and vice- versa. Priming of the seeds with various treatments was found effective to improve the seed quality in fresh as well as marginal seed lot i.e. one year seed lot. GA3 (50ppm for 6 hrs.) was found better priming treatment for enhancing the quality followed by hydration- dehydration (6hrs.) + 0.25% thiram treatments. All the priming treatments showed maximum effect on HJ541 followed by HC136. Therefore, from present investigation it is concluded that the standard germination, vigour indices and EC could be used as reliable predictors of seed quality because of easy, quick and accurate in their execution. Various seed priming treatments can be used for enhancing seed quality of marginal seed lot.