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ThesisItem Open Access H2O2-Induced Anti-Oxidant defense mechanism in wheat (Triticum aestivum L.) under salt and boron stress(College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Devi, Sarita; Angrish, RajivThesisItem Open Access Physiological and biochemical studies in mothbean [Vigna aconitifolia (Jacq.) Marechal] genotypes under soil moisture stress(CCSHAU, 2005) Sunil Kumar; Sharma, K.D.The study entitled “Physiological and biochemical studies in mothbean [Vigna aconitifolia (Jacq.) Marechal] genotypes under soil moisture stress” was conducted during Kharif season of 2004 at crop physiology research area of Agronomy Department CCS Haryana Agricultural University, Hisar. The experiment was laid out in factorial RBD with four replications. The treatment consisted of two environments of moisture stress (water stress and irrigated control) and four genotypes viz. RMO 40, RMO 257, MB 99-1 and CZM 10. Water stress decreased the maturity duration and profile moisture content consistently. Genotype RMO 40 was shortest in maturity duration and CZM 10 used maximum water. Development of moisture stress enhanced the phonological development significantly. Growth of mothbean in terms of plant height, dry matter accumulation, LAI, LAD, CGR, SLA, and RGR decreased with increase in moisture stress. However, root depth and SLW increased under moisture stress. All Plant water status parameters i.e. w, s, RWC and CTD decreased from morning to afternoon and with moisture stress. Gaseous exchange studies i.e. rate of photosynthesis, transpiration and stomatal conductance get reduced under water stress. Genotype MB 99-1 maintained higher biomass, plant water status and gaseous exchange under moisture stress. Biochemical metabolites i.e. chlorophyll, starch and protein content declined under water stress. However, free proline, total soluble carbohydrates, and free amino acids content showed sharp rise under moisture stress. Seed and biological yield reduced significantly by decreasing yield attributing characters i.e. number of effective pods, seeds per pod and test weight under water stress. Genotype MB 99-1 recorded highest seed yield and observed most drought tolerant in terms of having higher plant water status; gaseous exchange and biochemical metabolites.ThesisItem Open Access Effect of chromium toxicity and its possible amelioration by putrescine in cauliflower (Brassica oleracea var. Botrytis)(CCSHAU, 2006) Madaan, Sukham; Siddiqui, SaleemThe present investigations were conducted on cauliflower (Brassica oleracea var Botrytis) to study the accumulation of Cr in different plant parts and to evaluated the causes of Cr toxicity in relation to antioxidant defence mechanism and its possible amelioration by putrescine. In the first experiment plants were grown in dune sand treated with 0, 75 and 150 mM K2Cr2O7. Cr caused significant but variable decline in fresh weight, dry weight and calcium content of all the plant parts though it accumulated mainly in roots. No flowering was observed with Cr treatments. In second experiment the ameliorative effect of Purtrescine on oxidative metabolism in chromium treated cauliflower plant was studied. Cr increased the lipid peroxidation level. Decrease in protein and reduced glutathione content was also observed with the Cr treatment. The toxic effect of Cr was more severe at 200 mM. Putrescine was more effective in ameliorating the deleterious effects of Cr at lower concentration on plant growth, total soluble protein, lipid peroxidation. Among the antioxidant enzymes, putrescine had more positive effect on SOD enzyme and isoenzymes in Cr treated plants. However, putrescine also increased the activity of APX, GR and CAT enzyme and isoenzymes.