Effect of chromium toxicity and its possible amelioration by putrescine in cauliflower (Brassica oleracea var. Botrytis)
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Date
2006
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Publisher
CCSHAU
Abstract
The present investigations were conducted on cauliflower (Brassica oleracea
var Botrytis) to study the accumulation of Cr in different plant parts and to evaluated
the causes of Cr toxicity in relation to antioxidant defence mechanism and its possible
amelioration by putrescine.
In the first experiment plants were grown in dune sand treated with 0, 75 and
150 mM K2Cr2O7. Cr caused significant but variable decline in fresh weight, dry weight
and calcium content of all the plant parts though it accumulated mainly in roots. No
flowering was observed with Cr treatments.
In second experiment the ameliorative effect of Purtrescine on oxidative
metabolism in chromium treated cauliflower plant was studied. Cr increased the lipid
peroxidation level. Decrease in protein and reduced glutathione content was also
observed with the Cr treatment. The toxic effect of Cr was more severe at 200 mM.
Putrescine was more effective in ameliorating the deleterious effects of Cr at lower
concentration on plant growth, total soluble protein, lipid peroxidation. Among the
antioxidant enzymes, putrescine had more positive effect on SOD enzyme and
isoenzymes in Cr treated plants. However, putrescine also increased the activity of
APX, GR and CAT enzyme and isoenzymes.