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Effect of chromium toxicity and its possible amelioration by putrescine in cauliflower (Brassica oleracea var. Botrytis)
(CCSHAU, 2006) Madaan, Sukham; Siddiqui, Saleem
The present investigations were conducted on cauliflower (Brassica oleracea var Botrytis) to study the accumulation of Cr in different plant parts and to evaluated the causes of Cr toxicity in relation to antioxidant defence mechanism and its possible amelioration by putrescine. In the first experiment plants were grown in dune sand treated with 0, 75 and 150 mM K2Cr2O7. Cr caused significant but variable decline in fresh weight, dry weight and calcium content of all the plant parts though it accumulated mainly in roots. No flowering was observed with Cr treatments. In second experiment the ameliorative effect of Purtrescine on oxidative metabolism in chromium treated cauliflower plant was studied. Cr increased the lipid peroxidation level. Decrease in protein and reduced glutathione content was also observed with the Cr treatment. The toxic effect of Cr was more severe at 200 mM. Putrescine was more effective in ameliorating the deleterious effects of Cr at lower concentration on plant growth, total soluble protein, lipid peroxidation. Among the antioxidant enzymes, putrescine had more positive effect on SOD enzyme and isoenzymes in Cr treated plants. However, putrescine also increased the activity of APX, GR and CAT enzyme and isoenzymes.