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20151
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Subject: Molecular Biology and Biotechnology × End date: 2018 × Start date: 2010 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Micronutrient and SSR marker analysis of pearl millet [Pennisetum glaucam (L.) R. Br.] inbred lines
    (CCSHAU, 2015) Sangwan, Sonali; Yashveer, Shikha
    A set of thirty-six pearl millet inbred lines was used to study genetic variability and correlation analysis for seven morphological characters namely days to 50% flowering, plant height (cm), ear length (cm), ear diameter (cm), 1000 grain weight (g), grain yield per plant (g) and dry fodder yield per plant (g) along with micronutrient (Fe and Zn) status. The pearl millet inbred lines were evaluated in randomized block design with two replications in two environments during 2014. Analysis of variance for all the characters under study showed significant differences indicating the presence of genetic variability among the inbreds. The estimates of variability parameter for grain yield, contributing traits and Fe and Zn content revealed the high PCV value as compared to GCV value that suggests the role of environment in the expression of all these characters. An additive gene action was indicated for dry fodder yield per plant, grain yield per plant, Fe and Zn content by high to moderate heritability and genetic advance as per cent of mean values. Correlation analysis showed highly significant correlation between grain Fe and Zn content which signifies simultaneous improvement in the two traits. Grain yield per plant showed no significant negative correlation with Fe and Zn that suggests improvement in nutrient value without sacrificing yield. A set of 30 SSR primers was also used for molecular diversity assessment in 36 pearl millet inbred lines. Mean allele per locus and PIC obtained was 10.5 and 0.796, respectively. Cluster analysis distributed these genotypes into 9 clusters where 4 genotypes failed to fall in any of these clusters.