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Subject: Biotechnology and Molecular Biology × Author: Arora, Puneet × Start date: 1991 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Fingerprinting satawar (Asparagus racemosus) genotypes using RAPD markers
    (CCSHAU, 2005) Arora, Puneet; Dhillon, Santosh
    Satawar (Asparagus racemosus) is a medicinal plant growing in tropical climates and is useful in curing a wide array of ailments. This study was thus undertaken to prepare a DNA fingerprint database of selected varieties of Asparagus racemosus and to assess genetic diversity among them using randomly amplified polymorphic DNA (RAPD) markers. Twenty five RAPD primers were used to assess molecular polymorphism in fifteen Asparagus racemosus genotypes. A total of 211 amplified products were obtained out of which 50 were monomorphic and 161 were polymorphic. Average polymorphism across fifteen genotypes was found out to be 78.650%. For the genotypes tested, 5 to 17 bands were obtained, with an average of 10.55 bands per primer. The size of amplified fragments ranged from 230-2250 bp. Some primers also produced unique alleles in specific Asparagus genotypes which could be used to distinguish them. Analysis of this polymorphism profile, generated using suitable statistical programmes, grouped the fifteen genotypes into two major clusters at a similarity coefficient of 0.680. Varieties HAR-6 and Wild-3 were found out to be the most diverse and distant from other varieties. The second cluster again divided into two minor clusters, out-grouping the Nepali variety. The next large sub-cluster contained all the other genotypes. Varieties Wild-1 and Indian Yellow were genetically most similar. Genetic similarity matrices of the genotypes ranged from 0.630 to 1.00, indicating a moderate genetic variability among the genotypes. Wild-1 and Indian Yellow, showed a genetic similarity value of 1.00 while the genotypes HAR-1 and Wild-3 were found out to be genetically most diverse, at a value of 0.630. All other genotypes varied between these two extreme values. The results indicated that RAPD markers are efficient for identification of Asparagus racemosus genotypes and for determination of the genetic relationships among them. Fingerprint data obtained in this study can be further utilized in identification and development of improved Asparagus varieties.
  • Thumbnail Image
    ThesisItemOpen Access
    Fingerprinting satawar (asparagus racemosus) genotypes using RAPD markers
    (CCSHAU, 2005) Arora, Puneet; Dhillon, Santosh
    Satawar (Asparagus racemosus) is a medicinal plant growing in tropical climates and is useful in curing a wide array of ailments. This study was thus undertaken to prepare a DNA fingerprint database of selected varieties of Asparagus racemosus and to assess LITERATURE CITED -xivgenetic diversity among them using randomly amplified polymorphic DNA (RAPD) markers. Twenty five RAPD primers were used to assess molecular polymorphism in fifteen Asparagus racemosus genotypes. A total of 211 amplified products were obtained out of which 50 were monomorphic and 161 were polymorphic. Average polymorphism across fifteen genotypes was found out to be 78.650%. For the genotypes tested, 5 to 17 bands were obtained, with an average of 10.55 bands per primer. The size of amplified fragments ranged from 230-2250 bp. Some primers also produced unique alleles in specific Asparagus genotypes which could be used to distinguish them. Analysis of this polymorphism profile, generated using suitable statistical programmes, grouped the fifteen genotypes into two major clusters at a similarity coefficient of 0.680. Varieties HAR-6 and Wild-3 were found out to be the most diverse and distant from other varieties. The second cluster again divided into two minor clusters, out-grouping the Nepali variety. The next large sub-cluster contained all the other genotypes. Varieties Wild-1 and Indian Yellow were genetically most similar. Genetic similarity matrices of the genotypes ranged from 0.630 to 1.00, indicating a moderate genetic variability among the genotypes. Wild-1 and Indian Yellow, showed a genetic similarity value of 1.00 while the genotypes HAR-1 and Wild-3 were found out to be genetically most diverse, at a value of 0.630. All other genotypes varied between these two extreme values. The results indicated that RAPD markers are efficient for identification of Asparagus racemosus genotypes and for determination of the genetic relationships among them. Fingerprint data obtained in this study can be further utilized in identification and development of improved Asparagus varieties.