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  • ThesisItemOpen Access
    Effect of Orchard floor management practices on growth, yield and quality of Assam Lemon (Citrus limon Burm.)
    (AAU, Jorhat, 2017-07) Roy, Jyaosi; Hazarika, D. N.
    An experiment on “Orchard Floor Management Practices on Growth, Yield and Quality of Assam Lemon (Citrus limon Burm.)” was conducted during 2015- 2017 at Instructional cum Research Farm, Department of Horticulture, B.N. College of Agriculture, AAU, Biswanath Chariali with three objectives to study (i) Effect of different orchard floor management practices on growth, yield and quality of Assam Lemon (ii) Effect of different treatments of orchard floor management on weed growth in Assam Lemon plantation, (iii) Effect of different treatments of orchard floor management on different properties of soil in Assam Lemon plantation. Eight treatment combinations were laid out in Randomized Block Design with three replications in the field. The treatments were T1 (clean cultivation-removal of all weeds manually), T2 (mulching with dry leaves -10 cm thick), T3 (mulching with black polythene sheet -100 micron thickness), T4 (cover cropping with cowpea-sown at 6 months interval), T5 (green manuring with dhaincha -sown at 3 months interval), T6 (cover cropping with sweet potato), T7 (sowing of sesame in two seasons), T8 (control i.e. without any treatment). The results revealed that treatment T3 (mulching with black polythene sheet -100 micron thickness) showed maximum plant height (190.40 cm), number of laterals per primary shoot (5.66), number of leaves (32.19) and total leaf area (961.20cm2) with average number of hermaphrodite flowers (60.06) during April, 2016, fruit set (32.93 % in May, 2016), fruit retention (93.38 % in June, 2016), total number of fruits (810.18 per plant) harvested during one year of study and fruit yield (138.6 t/ha) with average fruit juice (27.55 %), titratable acidity (5.39 %), ascorbic acid content (39.31 mg/100 ml) were found to be highest in T3 (mulching with black polythene sheet) while average fruit length (10.38 cm), diameter (5.47 cm), weight (155.30 g), volume (155.68 cc), pulp-peel ratio (3.46) in T2 (mulching with dry leaves). Assam Lemon fruits could be stored for 14 days by wrapping with shrink film under refrigerated condition with retention of green colour and minimum physiological loss in weight. Total chlorophyll content was highest (1.25 mg g-1 of fresh weight) in T3 (mulching with black polythene sheet). Soil moisture content in different treatments showed less variation among the treatments. However, moisture content was highest in July, 2016. Bacterial population increased significantly in different treatments over the bacterial population recorded before application of treatments whereas fungal population was found to be non-significant.
  • ThesisItemOpen Access
    EFFECT OF ORGANICS ON GROWTH, YIELD AND QUALITY OF TURMERIC (Curcuma longa.L) cv. TALL CLONE
    (AAU, Jorhat, 2017-07) Ronya, Takar; Gautam, B. P.
    An experiment was carried out at the Instructional cum Research Farm, Department of Horticulture, B.N. College of Agriculture, AAU, Biswanath Chariali during 2016-17 to study the “Effect of organics on growth, yield and quality of Turmeric (Curcuma longa L) cv. Tall Clone”. Experiment was laid out in an organic block where experiment was continued last two years (15.02.2014 to 15.02.2016) under NICRA, All India Coordinated Research Project for Dryland Agriculture, BNCA. The experiment was laid out in Randomized block design with seven treatments replicated four times. The treatments were T1: Recommended dose of fertilizer, T2: In-situ incorporation of Dhaincha, T3: FYM @ 10 t ha-1, T4: Enriched compost ≈ FYM @ 10 t ha-1 in the furrow at planting, T5: Vermicompost ≈ FYM @ 10 t ha-1+ Microbial consortia as slurry for treatment of rhizome at planting, T6: Microbial consortia as slurry for treatment of rhizome with vermiwash (1:7) + vermiwash spray (1:7) every month up to seven months of planting, T7: FYM @ 10 t ha-1+ Microbial consortia as slurry for treatment of rhizome at planting. Healthy rhizomes were planted in each plot with spacing of 45cm x 25cm in 4th week of April 2016. The treatments positively responded to the manures in respect to morphological, Physiological and yield attributing characters. Among all the treatment, the recommended dose of fertilizer (T1) registered highest plant height (167.23 cm), number of leaves per plant (23.46), number of tillers per plant (3.90), leaf area of a single leaf (648.33cm2), leaf area per plant (15209.82cm2), leaf area index (12.42), Chlorophyll content index (32.14) and they were followed by treatment T7 and T4, these parameters were lowest in treatment T6. The highest light intensity of (77.50 105 lux) was observed in treatment T6. On the other hand, the highest number of mother rhizome (2.15), number of primary rhizome (6.54), number of secondary rhizome (16.79), number of tertiary rhizome (6.12), length of mother rhizome (11.16cm), length of finger rhizome (11.35), girth of mother rhizome (6.62cm), girth of finger rhizome (3.57cm), fresh weight of rhizome (424.25 g), dry weight of rhizome (72.08 g), and yield (335.25 q ha-1) were highest in T1 which were followed by T7 and T4 and these parameters were lowest in treatment T6. The results revealed that the curing per cent and curcumin content was significantly influenced by different treatments. Amongst all the treatments (T7) showed better performance in terms of curing percentage (18.09%) and curcumin content (3.62%). No serious pests and diseases were observed in the experimental plot during the course of experiment. The treatment T5 showed the maximum soil PH of 5.50 while treatment T1 exhibited the maximum organic carbon (0.90%), available soil N (328.16 kg ha-1), P2O5 (36.28 kg ha-1) and K2O (215.17 kg ha-1). Soil moisture content showed highest of 14.22% in T7 and the maximum moisture content was recorded in the month of July. Studies on economics of production showed that the maximum benefit: cost ratio of 3.15 was obtained in the treatment T7. From the present investigation, it can be concluded that after three years of cultivation organic manures (T7 and T4) could also perform equally with inorganic fertilizers in terms of growth and yield rather, perform better than inorganic in respect of quality of turmeric and was found to be optimum for economic cultivation of turmeric.
  • ThesisItemOpen Access
    FOLIAR APPLICATION OF GIBBERELLIC ACID ON GROWTH, FLOWERING, YIELD AND QUALITY ATTRIBUTES OF GERBERA (Gerbera jamesonii Bolus)
    (AAU, 2017-07) Jamir, Abenla B.; Mahanta, Pradip
    The experiment entitled “Foliar application of Gibberellic Acid on growth, flowering, yield and quality attributes of Gerbera (Gerbera Jamesonii Bolus).” was conducted in the Experimental Farm, Department of Horticulture, Assam Agricultural University, Jorhat during the year 2015-2016 and 2016-2017 with 7 treatments viz., T0 = Control, T1 = 50 ppm GA3, T2 = 100 ppm GA3, T3 = 150 ppm GA3, T4 = 200 ppm GA3, T5 = 250 ppm GA3, and T6 = 300 ppm GA3 in Randomized Block Design with 3 replications. The variety that was used during the experiment was Red Gem and the total area of the experiment was 99.75 m2. Pooled data over the two years indicated that the growth and flowering characters were significantly influenced by the application of different concentrations of Gibberellic acid. The highest value for most of the growth as well as flower attributing characters viz., plant height (48.36 cm), number of leaves per plant (32.12), leaf length (19.59 cm), leaf breadth (8.71 cm) leaf area (117.57 cm2 plant-1), leaf area index (2.32), number of suckers (15.10), number of flowers per plant (32.38), diameter of flower (11.58 cm), number of ray florets (44.63), number of disc florets (291.50), stalk length (44.13 cm), girth of flowering stalk (0.97 cm), longevity of flower (18.67 days), vase life (12.84 days), fresh weight of flower (12.74 gm) and dry weight of flower (2.49gm) were recorded by treatment T3 (150 ppm). The early flowering in terms of days taken from planting to flowering (70.27 days), days to visibility of flower bud (61.35 days), days to bud opening from visibility (4.85 days) and days to full bloom from bud opening (4.00 days) were also exhibited by treatment T3 (150 ppm), respectively. Similarly, T3 (150 ppm) also recorded the highest value for chlorophyll content (11.56 mg g -1). Available soil nitrogen (247.56 kg ha-1), available soil phosphorus (39.78 kg ha-1) and available soil potassium (119.42 kg ha-1) was recorded highest by treatment T0 (Control). Economics of cultivation revealed that the highest benefit cost ratio of 5.49 is obtained by T3 (150 ppm) closely followed by T4 (200 ppm) of 4.98. It can be concluded that GA 3 at 150 ppm has been able to positively influence the vegetative growth and flowering in gerbera resulting to be the best treatment among all the treatments taken during the course of this experimentation.
  • ThesisItemOpen Access
    Bunch Feeding of Nitrogen (N) and Potassium (K) in Banana, cv. Barjahaji (Musa AAA group)
    (AAU, Jorhat, 2017-07) Teron Millik, Thanuram; Baruah, Kartik
    The present investigation entitled “Bunch feeding of nitrogen and potassium in banana (Musa spp.) cv. Barjahaji” was carried out at the Experimental Farm, Department of Horticulture, Assam Agricultural University, Jorhat during March 2015 to June 2016. The experiment was laid out in Randomized Block Design and replicated thrice with nine treatments. The treatments comprised of T1 (500g fresh cowdung), T2 (500g fresh cowdung + 7.5g Urea), T3 (500g fresh cowdung + 7.5g KCl), T4 (500g fresh cowdung + 7.5g K2SO4), T5 (500g fresh cowdung + 7.5g KNO3), T6 (500g fresh cowdung + 7.5g Urea + 7.5g KCl), T7 (500g fresh cowdung + 7.5g Urea + 7.5g K2SO4), T8 (500g fresh cowdung + 7.5g Urea + 7.5g KNO3) and T9 (Control). The bunch attributes and quality parameters were studied. With respect to yield and yield attributing parameters, highest yield of (58.65 t ha-1), highest finger length (22.28 cm), finger girth (14.10 cm), volume of fingers (254.25 cc), weight of fingers (148.73 g), weight of second hand (4.00 kg), and bunch weight (19.00 kg), were observed with T7 (500g fresh cowdung + 7.5g Urea + 7.5g K2SO4). The highest pulp to peel ratio of 3.40 was recorded in T6 (500g fresh cowdung + 7.5g Urea + 7.5g KCl). Maximum rind thickness (3.56 mm) was observed in T3 (500g fresh cowdung + 7.5g KCl) and T5 (500g fresh cowdung + 7.5g KNO3) with regard to fruit quality parameters, less acidity (0.23), the highest total soluble solids (29.23 oBrix), total sugars (17.42%), reducing sugar (9.09%), and non-reducing sugar (8.33%), were observed in the treatment T7 (500g fresh cowdung + 7.5g Urea + 7.5g K2SO4). In respect to shelf life, bunch feeding with 500g fresh cowdung + 7.5g Urea + 7.5g K2SO4 had attained the highest shelf life (7.67 days). Bunch feeding of 500g fresh cowdung + 7.5g Urea + 7.5g K2SO4 was superior in terms of maximum gross returns (Rs. 169867.52 ha-1), net returns (416632.48 ha-1) and benefit cost ratio (2.45) were obtained. Hence bunch feeding of 500g fresh cowdung + 7.5g Urea + 7.5g K2SO4 can be recommended for banana cv. Barjahaji (AAA) for getting higher yields with best quality fruits of higher shelf life.
  • ThesisItemOpen Access
    EFFICACY OF BIOFERTILIZERS AND BIOREGULATORS IN MACROPROPAGATION OF BANANA
    (AAU, Jorhat, 2017-07) Narzary, Jonaki; Khangia, Bipin
    An experiment was conducted under shade net house at the experimental farm, Department of Horticulture, Assam Agricultural University, Jorhat, during October 2016 to May 2017 to study “Efficacy of biofertilizers and bioregulators in macropropagation of banana”. The experiment was laid out in Randomised Block Design with fourteen treatments comprising of two cultivars viz., V1 (Cheni Champa) and V2 (Kachkal) and seven different substrates consisting of T0 (Sawdust), T1 [Sawdust+ VAM (30 g)], T2 [Sawdust+ Trichoderma viride (30 g)], T3 [Sawdust + VAM (30 g)+ Trichoderma viride (30 g)], T4 [Sawdust+ IBA (2500 ppm)+ Azospirillum (30 g)], T5 [Sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)] and T6 [Sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] replicated three times. The results revealed that maximum growth and quality planting materials could be achieved by incorporation of additives like biofertilizers and bioregulators to the sawdust substrate. Among the treatments, V1T6 [Cheni Champa with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] had significant influence on time taken for bud initiation (23.22 days), number of secondary suckers (4.78), tertiary suckers (17.33) and percentage of suckers (90.33 %). It was observed that with the advancement of the hardening period, the growth characters of tertiary suckers increased from the initial stage to the end of the hardening. In terms of growth parameters of tertiary suckers, sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g) showed better result in both the cultivars. V2T6 [Kachkal with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] recorded significant influences on plant height (29.07 cm), stem girth (6.91 cm) and leaf length (35.63 cm) but V1T6 [Cheni Champa with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] gave best results for leaf breadth (15.33 cm), and weight of tertiary suckers (219.98 g), number of roots (19.56), root girth (1.45 cm) and the highest survival percentage (99.78 %). However, V1T4 [Cheni Champa with sawdust+ IBA (2500 ppm)+ Azospirillum (30 g)] recorded the highest number of secondary roots (84.89), total chlorophyll content (1.53 mg/g FW) and leaf relative water content (73.95 %). Economics of production revealed the highest B:C of 1.41 under V1T6 [Cheni Champa with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)]. Thus, application of biofertilizers and bioregulators exhibited positive impact on macropropagation of banana.
  • ThesisItemOpen Access
    POSTHARVEST STUDIES ON Lilium (ASIATIC LILY CV. BLACK OUT)
    (AAU, Jorhat, 2017-07) GOGOI, KONGKANA; Bora, Sunil
    The present investigation entitled “Postharvest studies on Lilium (Asiatic lily cv. Black Out)”, was carried out in the experimental laboratory of Department of Horticulture, Assam Agricultural University, Jorhat, during January-February, 2017. During the entire course of study, two experiments i.e. standardization of suitable pulsing and holding solutions were conducted on lily flowers. Lily stems were harvested when the first flower bud showed full colour. The first experiment was laid out in Completely Randomized Design with 6 treatments and 2 pulsing duration (12 and 24 hours), replicated 3 times. The pulsing treatments were P0 (distilled water), P1 (10% sucrose), P2 (100ppm AgNO3), P3 (10% sucrose+100ppm AgNO3), P4 (50ppm BA) and P5 (10% sucrose + 50ppm BA). After the treatments, each stem was maintained for postharvest evaluation in an individual 300 ml conical flask with 100 ml of 2% sucrose solution. Within each treatment, the number of buds per stem were kept constant as possible for ease of data recording. Pulsing of cut stems with 10% sucrose and 50ppm BA (P5) for 24 hours was observed to be best in terms of vase life (13.00 days), quality parameters and biochemical parameters viz., uptake of pulsing solution (6.21 ml/stem), higher rate of vase solution uptake and fresh weight, flower diameter (17.67 cm), total chlorophyll content (1.17 mg/g FW), relative water content (86.14%) and total soluble solid ( 4.45oBrix). However, P5 was statistically at par with P3, which gave the highest reducing sugar (5.18%) in tepals under 24 hours. The experiment on standardization of holding solutions was also laid out in Completely Randomized Design with 10 treatments replicated thrice. The holding treaments were T0 (distilled water), T1 (2% Sucrose + 100ppm 8-HQC+100ppm GA3), T2 (2% Sucrose + 200ppm 8-HQC+100ppm GA3), T3 (2% Sucrose + 300ppm 8- HQC+100ppm GA3), T4 (2% Sucrose + 20ppm AgNO3 + 150ppm Citric acid ), T5 ( 2% Sucrose + 30ppm AgNO3 + 150ppm Citric acid), T6 (2% Sucrose + 40ppm AgNO3 + 150ppm Citric acid ), T7 (2% Sucrose+ 500ppm Al2(SO4)3), T8 (2% Sucrose+ 500ppm Al2(SO4)3 +100ppm GA3) and T9 (2% Sucrose+ 500ppm Al2(SO4)3 + 150ppm Citric acid). Among the treatments, T5 resulted in highest vase solution uptake, fresh weight, delayed chlorophyll degradation, highest relative water content (84.24%), reducing sugar (5.53%) and minimum loss of dry weight (25.14%) which finally reduced the onset of petal senescence and recorded longest vase life (12.00 days). However, T2 exhibited the largest flower diameter (16.76 cm) and highest TSS content (4.95°Brix). Hence, considering the positive effects on post harvest quality, P5 (10% sucrose + 50ppm BA) for 24 hours and T5 (2% Sucrose + 30ppm AgNO3 + 150ppm Citric acid) could be considered as the best for pulsing and holding solution, respectively.
  • ThesisItemOpen Access
    GROWTH AND FLOWERING OF GERBERA UNDER HYDROPONICS
    (AAU, Jorhat, 2017-07) Handique, Dolly; Bora, Sunil
    An experiment was carried out at the experimental site, Department of Horticulture, Assam Agricultural University, Jorhat during September 2016 to May 2017 to study the “Growth and flowering of gerbera under hydroponics”. The experiment was laid out in two factor Completely Randomized Design with three replications comprising of eight different growth media viz., Control (untreated), T1 (Sand gravel), T2 (Coconut husk), T3 (Coco peat), T4 (Sand gravel+ Coconut husk), T5 (Sand gravel + Coco peat), T6 (Coconut husk+ Coco peat), T7 (Sand gravel+ Coconut husk+ Coco peat) and four duration of nutrient application viz., H1 (Once daily), H2 (Twice daily), H3 (Alternate days) and H4 (Two days interval). The study revealed that growth media significantly influenced the growth and development of gerbera plants. Maximum plant height (32.62cm),leaves per plant (15.17), leaf breadth (9.04cm), leaf area (108.18cm2), suckers per plant (4.63), early bud visibility (69.24 days), full bloom (17.79 days), flowers per plant (16.36), flower stalk length (51.90cm), flower diameter (8.59cm) and flower stalk dry weight (1.75g), self life (19.52 days), vase life (9.65 days), root fresh weight (10.72g), root dry weight (2.35g), root length (28.14cm), leaf relative water content (64.10%) and total chlorophyll content (1.405mg g-1) were obtained in T3. However, maximum leaf length (15.00cm), flower stalk fresh weight (10.68g) and roots per plant (14.62) was observed in T6. Regarding nutrient application, twice daily applied nutrient solution (H2) significantly influenced the plant height (31.29 cm), leaves per plant (14.60) leaf length (14.32cm), leaf breadth (8.33cm), leaf area (100.49cm2) suckers per plant (4.40), early bud visibility (75.43 days), full bloom (20.96 days), flowers per plant (14.46), flower stalk length (50.46 cm), flower diameter (8.23cm), flower stalk fresh weight (9.87g), flower stalk dry weight (1.42 g), self life (18.66 days) and vase life (9.02 days), root fresh weight (8.47g), root dry weight (1.63g) and root length (22.44cm), roots per plant (10.59), leaf relative water content (55.73 %) and total chlorophyll content (1.068mg g- 1).Treatment combination T3xH2 (Coco peat with twice daily applied nutrient solution) was found best for all the recorded characters except leaf length (15.67 cm),flower stalk fresh weight (11.48 g) and roots per plant (15.31 g)which were maximum in T6xH2 (Coconut husk+ coco peat and twice daily applied nutrient solution) while dry weight of flower (1.80 g) was maximum in T3xH3 (Coco peat and nutrient solution applied at alternate days). Economics of production resulted that the highest B:C of 4.01 observed in treatment combination T3xH2. Thus, it can be concluded that growth media and duration of nutrient application significantly affect the overall growth and flowering in gerbera. Coco peat with twice daily applied nutrient solution could be recommended for better effect in gerbera.
  • ThesisItemOpen Access
    Hydroponic systems for growth, development and quality flower production
    (AAU, Jorhat, 2017-07) Sarmah, Ruby; Bora, Sunil
    An investigation was carried out at Department of Horticulture, Assam Agricultural University, Jorhat during the year 2016 to 2017 to find out the best hydroponic system, EC of hydroponic nutrient solution and growing media for quality flower production in Tuberose, Gladiolus and Marigold. The experiment was laid out in two factor completely randomized design with 15 treatment combinations and with three replications comprising of five different levels of hydroponic systems viz. S1 (NFT or Nutrient Film technique), S2 (Water culture system) , S3 ( Aggregate or drip system with coco peat ), S4 (Aggregate or drip system with sand ), S5 (Aggregate or drip system with sawdust) and three different levels of hydroponic nutrient solution concentration viz. N1 (EC 1.0 dS/m), N2 (EC 1.5 dS/m), N3 (EC 2.0 dS/m) and one control (soil and water). Statistical analysis of the data revealed that most of the growth, flowering, root, bulb characters and physiological parameters were significantly influenced due to differences in systems of cultivation, media and nutrient concentrations (EC). Among the various levels of hydroponic systems, S2 (water culture) has shown the best results for most of the growth characters including root, bulb characters and physiological parameters in Tuberose. While, S1 (NFT) was significantly superior in most of the flower characters. The nutrient treatment N3 (EC 2.0 dS/m) has significantly improved all growth parameters except leaf length and plant height which were best in N1 (EC 1.0 dS/m). N3 treatment (EC 2.0 dS/m) was found to be the best in terms of flower and bulb characters including physiological parameters under study. However, the results of root characters were significantly superior in N1 (EC 1.0 dS/m). Hence, S1N3 (NFT + EC 2.0 dS/m) was found to be the best for Tuberose in terms of growth and flowering. In case of Gladiolus, most of the growth characters were found to be significantly superior in S2 (water culture). This treatment was also found to be effective in terms of root, corm and physiological parameters under study. In Gladiolus, a remarkable growth, flowering and corm production was observed in the nutrient treatment N2 (EC 1.5 dS/m). It can be concluded that, the treatment combination S2N3 (water culture + EC 2.0 dS/m) was the most suitable treatment for Gladiolus. In case of marigold, experimental data revealed that the growth and flower characters have shown significant superiority in S1 (NFT). Whereas, S2 (water culture) has shown the best results in root and physiological characters. Among the nutrient treatments, N2 (EC 1.5 dS/m) has shown the best response in marigold in most of the growth characters. Early emergence of bud and flower was noticed in N3 (EC 2.0 dS/m), whereas other flower characters were found to be significantly better in N2 (EC 1.5 dS/m). The root characters of marigold were expressed at its best in N1 (EC 1.0 dS/m) whereas, the physiological characters were found to be significantly improved in N3 (EC 2.0 dS/m). Thus, from the floriculture perspective conclusion can be drawn that treatment combination of S2N2 (NFT + EC 1.5 dS/m) is optimum for quality flower production of marigold. The experimental findings also deduced that for all crops under experiment, S3 (coco-peat) and S4 (sand) are the best media. However, S5 (sawdust) has shown the inferior result among media under study and it was at par with control in most of the observations. The statistical analysis of control vs. rest was shown to be highly significant in most of the observations under study. Thus, it can be concluded that different hydroponic systems, potting media along with their combinations among them exert effect on overall growth, flowering, root, bulb & other physiological parameters in different flowering crops.
  • ThesisItemOpen Access
    DEVELOPMENT AND QUALITY EVALUATION OF ‘READY-TO-COOK’ STEAKS FROM CATLA FISH (Catla catla)
    (AAU, Jorhat, 2017-07) SHARMA, PRIYAM
    Good quality fish is not available throughout the year to meet the demand of people preferring fish as a part of their daily diet. Processing of raw fish to make it pan-ready is also time-consuming and not convenient for consumers particularly for the urban areas where the people are leading a very fast paced life. The present investigation was carried out to develop a ‘ready-to-cook’ fish product (fish steak) from Catla fish with good nutritional quality and longer shelf life. The work was conducted at Fisheries Research Center and Department of Biochemistry and Agricultural Chemistry, Assam Agricultural University, Jorhat, during 2016-2017. Catla fish steaks treated with different preservatives viz., dry salt (T1), vinegar (T2), salt + turmeric (T3), salt+ turmeric+ vinegar (T4) and vacuum packed, sealed and stored for a period of 21 days at -18°C was studied for different biochemical properties at different interval of time. A control set was kept without any preservative under vacuum packed condition. Out of the different treatments, T4 was found the best treatment in view of the highest crude protein content (20.11%), overall acceptability (8.94), highest taste value (8.94) and flavor (8.44) and highest texture (8.81). Analysis of the microbial load revealed that all the treatments showed negative results, i.e., no microbial load in any of the treated samples were present. The control, although showed highest moisture content (78.30%), highest ash content (0.218%), highest fat content (2.63%), there was the presence of microbial load (7.08 cfu/g) and lowest protein content (17.90%) in all the replications. From the findings of the study an economically viable protocol for commercial production of ‘ready-to-cook’ fish steaks from Catla fish (Catla catla) that is marketable and usable up to 21 days of storage, can be developed for commercial production and development of entrepreneurship.