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ThesisItem Open Access EFFECT OF CHRONIC STRESS ON REPRODUCTIVE CYCLE AND EXPRESSION PATTERN OF RFRP-3 GENE IN ADULT FEMALE RATS(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) TZUDIR, AKUMMENLA; Banerjee, D; Bag, S.; Das, K.; Mukherjee, J.; Mondal, S.; Polley, S.The present study was conducted to analyze the effect of chronic stress on the reproductive cycle and expression pattern of the RFRP-3 gene in adult female rats. The experiments were performed on adult female Wistar rats which were divided into two groups viz. Control and the Stressed groups, and were housed individually in polypropylene cages. The rats of the Stressed group were subjected to 3 hrs. of restraint or immobilization stress daily in a modified rat restrainer for 28 days. The reproductive cycle of the female rats was monitored and the different stages of the oestrous cycle (proestrus, estrus, metestrus and diestrus) were determined through the visual assessment of the vagina of the rats and the vaginal exfoliated cell cytology. All rats from both the Control and the Stressed groups were tested for behavioural traits after the completion of the trial period. Different tests viz. open field locomotion test, Porsolt’s forced swim test, Light/Dark Transition test and the Elevated plus maze test were performed to observe the anxiety/depressive behaviour of the rats.The experimental animals of both groups were sacrificed at the end of the trial period. Plasma was separated from the collected blood samples and was estimated for haematological parameters (Hb, PCV, TEC, TLC and DLC), biochemical parameters (Blood glucose, Total antioxidant, T3, T4 and Cortisol) and reproductive hormones (LH, FSH, Progesterone and Estradiol). The tissue samples collected for histological studies were ovary and for the study of the expression of the RFRP-3 gene were brain (hypothalamus). During the visual assessment of the vagina, the vaginal opening of the rat appeared to be wide and moist and the tissues appeared pink during proestrus. During estrus, the vagina appeared similar to proestrus but was less pink and moist. In metestrus, a pale and dry vaginal opening was seen and during the proestrus, the vaginal opening was very moist, small and closed. The result of the vaginal cell cytology showed that a mixture of epithelial cells was seen in the proestrus. The estrus stage was characterized mainly by the presence of anucleated cornified (keratinized) cells. Metestrus shows the presence of leucocytes and some keratinized cells. Polymorphonuclear leucocytes were seen in diestrus. The behavioural analysis of the rats showed that the rats of the stressed group showed more depressive/anxiety-like behaviour when compared to the control group. The PCV and Hb of the stressed rats were highly significantly(P<0.01)lower than the control rats. The TLC and the TEC between the two groups were found to be not statistically significant (P>0.05). A significant (P<0.05) variation was observed in the blood glucose, T3, T4 and Cortisol between the two groups. The variation in the total antioxidant was found to be non-significant (P>0.05). A significant (P<0.05) variation was observed in the LH of the two groups. The variation in the mean with standard error of FSH, Estradiol (E2) and Progesterone of the two groups viz. the Control and the stressed group was found to be not statistically significant (p>0.05). A significant (P<0.05) increase in the expression of the RFRP-3 gene was seen in the hypothalamus of the stressed rat. The results lead us to conclude that chronic stress causes significant changes in the normal blood physiology of the rat. Chronic stress also alters the concentration of the reproductive hormones which confirms the relationship between stress-induced female infertility and the expression of RFRP-3.ThesisItem Open Access EFFECT OF DIETARY SUPPLEMENTATION OF VITAMIN E AND SELENIUM ON THE GROWTH, HEMATOBIOCHEMICAL, ENDOCRINE AND IMMUNE STATUS OF GROWER GHUNGROO PIGS(West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) Hussain, Rubayat; Mukherjee, J.; Das, P.K.; Banerjee, Dipak; Batabyal, Subhasis; Bag, SadanThe focus of the present investigation was to highlight the findings of effectiveness of dietary supplementation of vitamin E and selenium in grower Ghungroo pigs on growth, hematobiochemical, endocrine, immunity and antioxidant status. Eighteen healthy female grower Ghungroo pigs (Susscroffa domestica) of 2-3 months were selected randomly for the experiment and divided into three groups. The control group (n=6) fed only the basal diet, animals of treatment-1 (T1) group (n=6) were supplied with vitamin E (in form of α tocopherol acetate) @11IU/Kg feed and animals of treatment-2 (T2) group of animals (n=6) were supplemented with selenium (as sodium selenite) @0.15mg/Kg feed daily as per NRC recommendations. The body weight, morphometric parameters of growth and physiological responses were recorded at the interval of 15 days. Blood samples were collected from all the experimental animals on day 15, day 30, day 45 and day 60 of supplementation. Vitamin E and selenium were estimated in the plasma. Hematological parameters were evaluated as per standard procedures. Blood biochemical, endocrine (cortisol, T3 and T4) and total antioxidant status were evaluated by commercially available kits as per manufacturer’s protocol. In vitro phagocytic activity and lymphocyte proliferation response were evaluated by colorimetric NBT and MTT assay respectively. The supplementation of vitamin E and selenium significantly (P<0.01) increased plasma vitamin E and selenium concentration in grower Ghungroo pigs from 15th day of supplementation and persisted till the end of experiment. The supplementation of vitamin E and selenium did not have any significant effect on the growth related morphometric parameters in Ghungroo pigs. The supplementation of vitamin E increased haemoglobin and MCH concentration whereas, selenium supplemented pigs showed higher PCV, MCV, TEC and TLC. The supplementation of vitamin E significantly increased plasma glucose and decreased AST and ALT whereas, selenium supplementation resulted higher total protein in experimental pigs. Vitamin E-treated group had a considerably greater T3 and T4 level and lower cortisol level than the control and selenium-treated groups. Vitamin E supplementation improved the immune status of the growing Ghungroo pigs in terms of in vitro lymphocyte proliferation response, phagocytic activity of neutrophils along with increased total antioxidant status. In conclusions, the present investigation depicted that dietary supplementation of vitamin E had beneficial effect on the immunity and antioxidant status in grower Ghungroo pigs without affecting normal growth and physio-biochemical functions.ThesisItem Open Access Effects of dietary turmeric and lemon essential oil on weaning and post-weaning performances of Ghoongroo piglets(West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) DAS, ANIL KUMAR; Das, P.K.; Sanyal, Sagar; Mukherjee, J.; Batabyal, Subhasis; Patra, A.; Naskar, S.ThesisItem Open Access Assessment of Cellular and Biochemical Responses of Fish Collagen with Ion-Doped Bioactive Glass Electrospun Fibers/ Mats on Diabetic Wound Healing in Rabbit Model(West Bengal University of Animal & Fishery Sciences, Kolkata, 2021) Jana, Sonali; Ghosh, Prabal RanjanThesisItem Open Access Expression Profile of Rfamide-Related Peptide-3 (RFRP-3) During Different Stages of Reproductive Cycle in Rat(West Bengal University of Animal & Fishery Sciences, Kolkata, 2021) Mohapatra, Smruti Smita; Mukherjee, JoydipThesisItem Open Access Isolation and characterization of bovine mesenchymal stem cells(Kolkata, 2019) Debbarma, Parishma; Das, Dr. Kinsuk; Das, Dr. S. K.; Banerjee, Dr. Dipak; Mukherjee, Dr. Joydip; Mandal, Dr. Arun Kumar; Bag, Dr. SadhanThesisItem Open Access Pre pubertal physio - biochemical and endocrine profiles in relation to growth of black bengal goat ( capra hircus ) rearing under free range system .(Kolkata, 2017) Perveen, Shagufta; Das, Prof. P. K.; Ingole, Prof. S. D.; Ghosh, Dr. P. R.; Banerjee, Dr. D.; Mukherjee, Dr. J.; Batabyal, Prof. S.; Naskar, Dr. S.ThesisItem Open Access PHYSIO-BIOCHEMICAL PROFILE OF PIG MUSCLE IN RELATION TO MONOCARABOXYLATE TRANSPORTER (MCT) GENE(Kolkata, 2014) Th ulasiraman, P.; Banerjee, Dr. D.; Mondal, Dr. M.; Das, Dr. P. K.; Ghosh, Dr. P. R.; Mukherjee, Dr. J.; Das, Dr. B. C.; Batabayal, Dr. S.; Das, Dr. A. K.ThesisItem Open Access STUDIES ON CERTAIN PHYSIO·BIOCHEMICAL CHANGES ASSOCIATED WITH ADRENO·CORTICAL INSUFFICIENCY IN ADRENALECTOMIZED BLACK BENGAL GOAT (Capra hircus) WITH SPECIAL REFERENCES TO MINERAL STATUS(Kolkata, 2004) Das, Kinsuk; Roy, Prof. (Mrs.) S.; Ghosh, Dr. P. R.; Das, Dr. P. K.; Batabyal, Dr. S.; Samanta, Prof. G.; Ghosh, Dr. D.
