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ThesisItem Open Access APPLICATION AND EVALUATION OF MESENCHYMAL STEM CELLS ON WOUND HEALING IN RAT(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) MAHESH A, KUMARA DATTHA; Pradhan, S.; Mondal, S.; Das, K.; Bag, Sadhan; Dey, SamirThe primary goal of this study is to assess the wound healing effect of mesenchymal stem cells and condition media in xenogeneic transplantation on rat skin full thickness excisional wounds. MSCs derived from bovine umbilical cord were isolated and cultured using explant and enzymatic methods. The MSC isolation technique was standardised using enzymatic digestion, as was the explant method. Because of their migratory properties, MSCs emerged from explants by day 4 and reached 70-90% confluence by day 10. MSCs were digested with collagenase (1mg/ml) for 4 hours at 370C before being plated and cultured, reaching 90% confluence after 72 hours. MSCs exhibited strong plastic adherence and fibroblast-like morphology. MSCs were cultured in vitro and differentiated into osteocytes, chondrocytes, and adipocytes according to International Society for Cell Therapy and Mesenchymal Stem Cell Committee guidelines. Xenogeneic bovine UC-MSC and bovine UC-MSC derived condition media were transplanted into two treatment groups and compared to positive, negative (vehicle), and wound healing ointment groups. A full thickness (10mm) skin punch biopsy was performed on both sides of the shoulder region, and MSC and CM were injected at the wound's periphery. Wound healing was evaluated using wound contraction (up to day 14), gross morphological evaluation (up to day 7), cytological examination of the wound bed area (up to day 7), and histo-morphological evaluation of skin samples collected on days 7, 14, 21, and 28. Daily photographs were taken from the same height, and wound closure was accelerated in the MSC and CM treated groups. Gross morphological parameters were scored, and it was discovered that all wounds in all experimental groups were grossly healthy until day 7, with enhanced granulation tissue noted in MSC and CM treated groups. Cytological analysis revealed a significant increase in mononuclear infiltration after 72-96 hours in the CM and MSC groups. Overall, histomorphological observations revealed that MSC and CM treated groups had significantly accelerated wound closure, advanced re-epithelisation and neovascularisation, mature extra-cellular matrix deposition with better collagen arrangement, and reduced scarring. Every parameter was scored and statistically evaluated.ThesisItem Open Access ROLE OF CURCUMINOID AND SESAMOID OIL IN COLON CANCER DEVLOPMENT AND PROGRESSION(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) SENAPATI, SANJIB; Hansda, R.N.; Mukhopadhayay, S.K.; Pradhan, S.; Mondal, Samiran; Dey, SamirThesisItem Open Access MULTIPLE DOSE EXPOSURE STUDY OF Psidium guajava Linn. ON EXPERIMENTALLY INDUCED INFLAMMATORY BOWEL DISEASE IN ANIMAL MODEL(West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) MEHRA, SANDEEP; Hansda, R.N.; Kumar, Deepak; Mukhapadhayay, S.K.; Mondal, Samiran; Pradhan, Saktipada; Batabyal, KunalThe current study attempted to screen Psidium guajava leaves for their influence on experimentally produced inflammatory bowel illness (IBD). Inflammatory bowel disease (IBD) is a prevalent chronic inflammatory gastrointestinal condition. Crohn's disease (CD) and ulcerative colitis are the two most common kinds of IBD (UC). The anti inflammatory and antioxidant properties of Psidium guajava leaves were investigated in DSS-induced IBD. The experiment was performed using Dextran sulphate Sodium (DSS) 2 % DSS is added to the drinking water for 7 consecutive days except in negative controle. Aquous extract of P. guajava will be given for 28 days [from day 1 to day 28 with DSS(7days )] to know the antiinflammatory and antioxidant properties the rats will be divided into following namely group I: negative control, Group II: positive control, Group IlI: P. guajava leaf extract 30mg/kg, Group IV: P. guajava leaf extract 60 mg/kg and Group V: P guajava leaf extract 120mg/kg. Haemoglobin,TEC, TLC, DLC, Total Platelet Count, and other common haematological parameters and serum biochemistry such as ALT, AST, Kidney Function Test (crt.and urea) total protein and serum electrolyte sodium were assessed and found maximum of test shows significance difference. In cytology-based analysis, anal cytology for representative group of positive control(dss 2%) were taken every day for 7 consecutive days with using swab method in anal rectal region. In histomorphological of small intestine found that mucosal ulcer ,erosion,necrosis amd infiltration of inflammatory cell.In kidney they have been normal architecture with focal. In liver no histopthological changes except glycogen accumulation in liver. In gross pathology inflammatory nodule is seen on 1st and 2nd week of experiment. Histopathological changes of liver, kidney and intestine were significantly reduced by aqueous extract of Psidium guajava in last week of experiment. Therefore psidium guajava has a good protective potential in inflammatory bowel disease.ThesisItem Open Access Epidemiological, Pathological & Molecular Studies of Inclusion Bady Hepatitis in Broiler(West Bengal University of Animal & Fishery Sciences, Kolkata, 2021) Shankar, Karnam Shiv; Mukhopadhayay, S.KThesisItem Open Access Studies on prevalence, pathology haemato - biochemical changes of canine demodicosis in and arround kolkata(Kolkata, 2002) Das, Liton; Mukhopadhay, Dr. S .K.; Som, Dr. T. L.; Bhowmiki, Prof. M. K.; Dasgupta, Prof.C. K.; Deb, Prof. (Mrs.) P.; Roy, Prof. (Mrs.)ThesisItem Open Access Studies on pathology of spontaneously occuring renal disorders in garole sheep in west bengal(Kolkata, 2003) Pramanik, Dipak; Deb, Prof. (Mrs.) P.; Som, Dr. T. L.; Bhowmik, Prof. M. K.; Basak, Prof. D. K.; Mukhopadhyay, Dr. S. K.ThesisItem Open Access Pathological studies of Avian inflammation with special reference to Basophil hypersensitivity in the skin of chicken(Kolkata, 2005) Prasad, Ram Anuj; Mukhopadhayay, S.K.; Sinha, Prof. Binay Kumar; Basaak, Prof. D. K.; Deb, Prof. (Mrs.) Pratima; Chakrabarti, Dr. Amlendu; Nandy, Dr. P.R.ThesisItem Open Access STUDIES ON PATHOGENESIS OF Cryptococcus species ISOLATED FROM ENVIRONMENTAL SOURCES USING MOLECULAR TECHNIQUES(Kolkata, 2017) Banerjee, Aniruddha; Pradhan, Dr. S; Panda, Prof. S.K.; Mukhopadhayay, Dr. S. K.; Patra, Dr. N. C.; Debnath, Dr. C.; Baidya, Dr. S.ThesisItem Open Access Neuropatological studies of rats following multiple exposure to acetamiprid and associated stress(Kolkata, 2015-01) Mondal, Samiran; Mukhopadhyay, Dr. S. K.; Panda, Prof. S. K.; Batabyal, Dr. S.; Patra, Dr. N. C.; Pradhan, Dr. S.; Sar, Dr. T. K.
