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2022 - 20234
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Subject: Aquatic Animal Health Management ×

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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    EFFECT OF YAPAVA TRIPLINERVIS IN LABEO ROHITA AGAINST BACTERIAL INFECTION WITH SPECIAL EMPHASIS ON ULCER CAUSED BY AEROMONAS HYDROPHILA
    (West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) SAHA, ANKITA; Dash, Gadadhar; Pramanik, Koushik; Abraham, T.J.; Sau, S.K.; Karmakar, S.
    Motile Aeromonas Septicemia, caused mainly by Aeromonas sp. is an economically significant problem in freshwater water fish farming industry. The goal of the study is to determine the inhibitory effect of Ayapana triplinervis against Aeromonas hydrophila and determining the efficacy of A. triplinervis against ulcer disease of fish caused by A. hydrophila. In bacterial susceptibility test 2.5 μl of Ayapana triplinervis leaf chloroform extract gave zone of inhibition through disc diffusion assay and the minimum inhibitory concentration was determined through agar dilution method. The minimum inhibitory concebtration was 6% Ayapana triplinervis leaf powder (ALP) against A. hydrophila present in 0.1 ml of 108 CFU/ml through agar dilution method in petri plate. The efficacy of Ayapana triplinervis was evaluated by treating the Aeromonas hydrophila infected Labeo rohita with Ayapana triplinervis leaf powder (ALP) admixed with feed at different doses. The Ayapana triplinervis feed treatment at doses of 10 gm/kg feed and 100gm/kg feed gave better results and were effective in reducing mortalities in diseased Labeo rohita. Histopathological findings suggested marked improvement in the gill, spleen, skin, kidney and liver tissues of Labeo rohita infected with Aeromonas hydrophila upon Ayapana triplinervis treatment at higher doses for 14 consecutive days. Ayapana triplinervis also restored serum biomarkers like glucose, creatinine, calcium, ALT and AST to normal range after 14 days treatment with Ayapana triplinervis leaf powder at the doses of 10gm/kg feed and 100gm/kg feed. The hematological properties like total erythrocyte count, haemoglobin concentration, packed cell volume and total leucocyte count were also normal in Labeo rohita challenged with alpha haemolytic and antigenic Aeromonas hydrophila after 14 days Ayapana treatment at the doses of 10gm ALP/kg feed and 100 gm ALP/feed. This study has been suggested to treat ulcer disease in Labeo rohita caused by Aeromonas hydrophila by Ayapana triplinervis leaf (in powder form) as an effective herbal therapeutic agent.
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    ThesisItemOpen Access
    INFLUENCE OF CAGE AQUACULTURE ON WATER QUALITY PARAMETERS IN SUNDARBAN ESTUARY, WEST BENGAL
    (West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) TANDEL, MANTHANKUMAR AMRUTLAL; Das, B.K.; Saboo, Amiya Kumar; Rout, S. K.; Karmakar, S.; Chowdhury, S.; Sau, S.K.
    The present investigation was undertaken to assess the influences of cage culture on the water quality parameters at coastal estuarine areas of Sundarban, West Bengal, to investigate the influence of the cage on threw surrounding waterbody, to assess the length and weight of Asian Seabass and to study the plankton diversity. This study was carried out on cage site (S1), 50 m away (S2) and 100 m away from cages site (S3) for a period of six months from April, 2022 to September, 2022. The various physio-chemical parameters such as water temperature, air temperature, pH, dissolved oxygen (DO), salinity, total hardness, total alkalinity, and free CO2 were analyzed in the water samples. Water temperature values ranged from 29.2 to 33.1 ºC in cage culture and other sites. Air temperature values ranged from 25.7 to 36.7 ºC in cage culture and other sites. The total hardness values ranged from 1067 to 1749.3 mg/l as CaCO3. The total alkalinity values ranged from 113.7 to 178.3 mg/l at cage sites. The dissolved oxygen (DO) values ranged from 4.2 to 6.2 mg/l at cage and control sites. All the water quality parameters analysed were observed within the permissible range for fish culture and drinking purpose except hardness. The optimum water quality characteristics observed at cage culture site clearly showed that the small cage farming in the reservoir does not have major environmental influences on the water and sediment quality. The cage of Asian Seabass was of circular shape with diameter of 4.4 m, depth of 2.5 m having area of 15.2 m2 and volume of 38 m3. Total 380 numbers of Asian Seabass were stocked in the cages (stocking density 25 nos. / m2 by area and 10 nos. / m3 by volume). The feeding was not done because of natural feed availability. The length-weight relationship of Lates calcarifer was based on 60 samples. The length weight relationship for L. calcarifer was found as log log W = log 0.0307 + 2.7629 log L. The deviation of 'b' value from the ideal value of 3 (cube) was tested by the 't' test (-0.5106). It was observed that the fish exhibited isometric growth for L. calcarifer during the study period. The 'b' value of L. calcarifer (Asian Seabass) varied from 2.2003 to 3.0362. K values of L. calcarifer ranged from 0.7077 to 1.0218 during the study period. The different plankton from different phytoplankton division like Bacillariophyceae, Chlorophyceae, Cyanophyceae and zooplankton were found from the cage site and monthly variation can be seen during the study period.
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    ThesisItemOpen Access
    Toxicological Impact of 4-tert-butylphenol On Labeo rohita (Hamilton, 1822) Fingerling Through Biomarker Approaches
    (West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) Mandal, Sulav; Rout, S. K.; Tripathy, M.K.; Trivedi, R.K.; Karmakar, S.; Das, S.K.; Mali, P.
    The present study was carried out to investigate the impact of acute and chronic exposure of 4-tert-Butylphenol on behaviour, histopathology, haematology and biochemical parameters of Labeo rohita (Hamilton, 1822). The 96 h LC50 of 4-tert- Butylphenol for Labeo rohita was estimated to be 4.45 mg/l. Further, sub-lethal 4-tert- Butylphenol exposure to 1/25th (0.1816 mg/l), 1/50th (0.0908 mg/l) and 1/100th (0.0454 mg/l) of 96 h LC50 concentration for a period of 30 days lead to decrease in total erythrocyte count and haemoglobin content while total leucocyte count increased significantly (p<0.05) as compared to control. Among the biochemical parameters, blood glucose level, Serum Glutamate Oxaloacetic Transaminase, Serum Glutamate Pyruvate Transaminase level increased but, total serum protein, albumin and globulin level decreased significantly (p<0.05) compared to control. Significant alterations occurred in the gill and liver tissues of 4-tert-Butylphenol exposed groups. A significant (p<0.05) rise in the activity of catalase and super-oxide dismutase in gill and liver tissues and inhibition of acetyl cholinesterase activity was observed on 15th and 30th day of exposure which was dependent on both the concentration of 4-tert-Butylphenol and duration of exposure. A significant (p<0.05) high activity of glutathione-Stransferase in gill and liver tissue was also observed in 4-tert-Butylphenol exposed groups in comparison to control group during the experimental period. The study showed that the long-term sub-lethal exposure of 4-tert-Butylphenol to fish lead to several physiological alterations such as enzymatic scavenging of oxygen radicals and the normal neurological functions. With increasing anthropogenic activity, the study provides convincing evidence for the necessity of regulated use and safer disposal of 4- tert-Butylphenol to the environment.
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    ThesisItemOpen Access
    EFFECTS OF SELECTED CARBON AND NITROGEN RATIO IN BIOFLOC REARING SYSTEM FOR NILE TILAPIA (OREOCHROMIS NILOTICUS) AND ASIAN STINGING CATFISH (HETEROPNEUSTES FOSSILIS)
    (West Bengal University of Animal & Fishery Sciences, Kolkata, 2023) Mali, Prasenjit; Dash, Gadadhar; Abraham, T.J.; Das, Sudhir Kumar; Chowdhury, Supratim; Ghosh, Swagat
    The biofloc technology (BFT) has gained popularity in aquaculture sector during last decade due to its ability towards bio-security, growth, feed utilization, health and cost effectiveness. To understand the present scenario of BFT in West Bengal, a preliminary survey was conducted first with the help of randomly selected 110 biofloc farmers from 13 districts. Oreochromis niloticus (Nile tilapia) was found highest reared species followed by others including Heteropneustes fossilis (Singhi). Majorities were failed to achieve expected profit due to mortalities. Only, 13.6% farmers were found satisfied. Synchronizing carbon to nitrogen (C:N) ratios with microbial dynamics was found the major gap. In this study the effects of different C:N ratios in jaggery based BFT on water quality, growth, feed performances, welfare, body composition of both O. niloticus and H. fossilis were investigated in a separate 120 d and 180 d trial, respectively. A completely randomized design (312.5 g/m3 in 4000 l) was used in triplicates, where a control group without biofloc and three BFT Tilapia (0.5±0.05 g) groups were reared in different C:N ratios i.e., 15:1(CN15), 20:1(CN20) and 25:1(CN25), respectively. Similarly, Singhi (5±0.05 g) were reared in control and BFT with C:N ratios of 8:1(CN8), 12:1(CN12) and 16:1(CN16) in another randomized design (2.33 kg/m3 in 1500 l) in triplicate. The fish were fed daily with commercial diet at 5-2% of their body weight. BFT groups were found better in all aspects than control. Highest survivability was found in lower C:N ratio groups. Highest final body weight and biomass were recorded as 160.35±2.16 g and 366.31±3.54 kg in CN15 of Tilapia, and 57.65±1.53 g and 38.92±1.21 kg in CN12 of Singhi groups, respectively. Feed performances among BFT groups for both were found insignificantly difference. Biofloc proximate showed higher protein and ash content with increasing C:N ratios. The CN15 followed by CN20, and CN12 followed by CN8 showed better results on assessment of stress and liver biomarkers, hematology, innate immunity, antioxidant properties, histological status of liver and intestines. Decreased dissolve oxygen, pH, ammonia nitrogen, nitrite and nitrate, and increased total suspended solids, floc volume and total heterotrophic count were recorded with increasing C:N ratios. Considering all aspects the C:N ratios of 15:1 and 12:1 were found most suitable for biofloc culture of Tilapia and Singhi, respectively.