EFFECT OF YAPAVA TRIPLINERVIS IN LABEO ROHITA AGAINST BACTERIAL INFECTION WITH SPECIAL EMPHASIS ON ULCER CAUSED BY AEROMONAS HYDROPHILA
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Date
2022
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West Bengal University of Animal & Fishery Sciences, Kolkata
Abstract
Motile Aeromonas Septicemia, caused mainly by Aeromonas sp. is an economically significant problem in freshwater water fish farming industry. The goal of the study is to determine the inhibitory effect of Ayapana triplinervis against Aeromonas hydrophila and determining the efficacy of A. triplinervis against ulcer disease of fish caused by A. hydrophila. In bacterial susceptibility test 2.5 μl of Ayapana triplinervis leaf chloroform extract gave zone of inhibition through disc diffusion assay and the minimum inhibitory concentration was determined through agar dilution method. The minimum inhibitory concebtration was 6% Ayapana triplinervis leaf powder (ALP) against A. hydrophila present in 0.1 ml of 108 CFU/ml through agar dilution method in petri plate. The efficacy of Ayapana triplinervis was evaluated by treating the Aeromonas hydrophila infected Labeo rohita with Ayapana triplinervis leaf powder (ALP) admixed with feed at different doses. The Ayapana triplinervis feed treatment at doses of 10 gm/kg feed and 100gm/kg feed gave better results and were effective in reducing mortalities in diseased Labeo rohita. Histopathological findings suggested marked improvement in the gill, spleen, skin, kidney and liver tissues of Labeo rohita infected with Aeromonas hydrophila upon Ayapana triplinervis treatment at higher doses for 14 consecutive days. Ayapana triplinervis also restored serum biomarkers like glucose, creatinine, calcium, ALT and AST to normal range after 14 days treatment with Ayapana triplinervis leaf powder at the doses of 10gm/kg feed and 100gm/kg feed. The hematological properties like total erythrocyte count, haemoglobin concentration, packed cell volume and total leucocyte count were also normal in Labeo rohita challenged with alpha haemolytic and antigenic Aeromonas hydrophila after 14 days Ayapana treatment at the doses of 10gm ALP/kg feed and 100 gm ALP/feed. This study has been suggested to treat ulcer disease in Labeo rohita caused by Aeromonas hydrophila by Ayapana triplinervis leaf (in powder form) as an effective herbal therapeutic agent.