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ThesisItem Open Access EFFECTS OF CINNAMON OIL AS AN ALTERNATIVE TO ANTIBIOTIC GROWTH PROMOTER IN BROILERS(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand, 2021) Solanki Devasibhai M.; Dr. C. J. DaveThe present investigation was planned to evaluate the effects of cinnamon oil as an alternative to antibiotic growth promoter in broilers. A total of 144 broiler chicks were randomly allocated to six experimental groups, each of which consisting of four replicates and each replicate consisting of six chicks. The group Ⅰ chicks were served as a control and given basal diet without administration of any treatments. Chicks of group Ⅱ were given chlortetracycline as an antibiotic growth promoter (1 g/Kg feed) as a standard control. Chicks of group Ⅲ were given cinnamon oil (400 mg/kg feed) as a treatment control. Chicks of group Ⅳ were served as a negative control and given basal diet and challenged with E. coli at the dose rate of 1.0 ml (1.5×108 CFU/ml) orally on 14th day. Chicks of groups Ⅴ were given chlortetracycline (1g/kg feed) with E. coli (1.5×108 cfu/ml) orally on 14th day. Chicks of group Ⅵ were given cinnamon oil (400 mg/kg feed) with E. coli (1.5×108 cfu/ml) orally on 14th day of experiment.ThesisItem Open Access ETIOPATHOLOGICAL STUDY ON EGG PERITONITIS IN COMMERCIAL LAYER(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2021) Sheth Aaftabhussain Liyakatali; Dr. D. J. GhodasaraThe present study was carried out to know the mortality pattern, etiology, gross and microscopic lesions of the egg peritonitis in commercial layer birds. The study comprised of flock wise mortality, gross and histopathological lesions, isolation, identification, biochemical characterization, antiobiogram of probable causative bacteria and molecular detection of isolated organisms.ThesisItem Open Access PATHO - EPIDEMIOLOGICAL AND MOLECULAR STUDIES OF CHICKEN INFECTIOUS ANEMIA IN COMMERCIAL LAYERS(Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 2021) Balaji M. Kondre; Dr. D. J. GhodasaraThe present research work was carried out on chicken infectious anaemia in commercial layers in an around Anand (Gujarat). The Patho - epidemiological study comprised of mortality pattern, gross and histopathological lesions along with haematological and biochemical study. Molecular study comprised of identification of viral DNA by targeting VP1, VP2 and VP3 gene of chicken infectious anaemia virus by PCR and further characterization of VP1 and VP2 gene by sequencing.ThesisItem Open Access ETIOPATHOLOGICAL STUDY OF COLISEPTICEMIA WITH COINFECTION OF LOW PATHOGENIC AVIAN INFLUENZA AND MYCOPLASMA IN BROILERS(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Pradhnya Choudhari; Dr. D. J. GhodasaraThe present study was carried out to know the mortality pattern, gross and microscopic lesions of the colisepticemia in broiler chicken along with molecular detection of LPAI and avian mycoplasma in colisepticemia affected flocks. The study comprised of farm wise and age wise outbreaks due to Escherichia coli infection, gross and histopathological lesions of different organs, isolation, identification, biochemical characterization, antibiogram profile and molecular detection of virulence associated genes of Escherichia coli. Further, molecular detection of LPAI (H9N2) by RT-PCR and avian mycoplasma (Mycoplasma gallisepticum and Mycoplasma synoviae) by PCR in broilers affected with colisepticemia. Studies on the farm wise and age wise outbreak of colisepticemia among thirty broiler farms having total population of 1,71,317 birds of second to fifth week of age showed an average mortality of 1.89 per cent ranging between 0.32 and 6.75 per cent. The age wise outbreaks of colisepticemia revealed mortality 1.65, 1.97, 2.21 and 1.71 per cent during second, third, fourth and fifth week of age respectively. The outbreaks were higher in number during third, fourth and fifth weeks being the susceptible age for colisepticemia.ThesisItem Open Access ETIOPATHOLOGICAL STUDY OF SWOLLEN HEAD SYNDROME IN BROILERS(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Momin Sohilabbas G.; Dr. D. J.GhodasaraThe present study was carried out to know the mortality pattern, etiology, gross and microscopic lesions of the swollen head syndrome in broiler birds. The study comprised of farm wise mortality, gross and histopathological lesions, isolation, identification, biochemical characterization of possible causative bacteria, molecular detection of Avian metapneumovirus (aMPV) by RT-PCR and detection of Avian mycoplasma (M. gallisepticum and M. synoviae). Studies on the farm incidence and mortality among twenty affected broiler flocks having total population of 84,050 birds of second to fifth week of age showed an average mortality rate of 1.63 per cent ranging from 0.34 per cent to 7.50 per cent. The week wise average mortality due to swollen head syndrome was 0.80, 1.62, 1.24 and 2.43 per cent respectively during second, third, fourth and fifth weeks of age.ThesisItem Open Access PATHO - EPIDEMIOLOGICAL AND MOLECULAR IDENTIFICATION OF CHICKEN ANAEMIA VIRUS IN BROILERS(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Kabariya Digjay Vasantbhai; Dr. C. J. DaveChicken infectious anemia (CIA) is known to be an emerging, immunosuppressive and highly contagious disease that affects young broiler chickens from 3 weeks of age. The disease's causative agent is Chicken anaemia virus (CAV), which belongs to the genus Gyrovirus of Circoviridae family. In commercial broilers, there is a better need to diagnose the sub-clinical type of CIA in the field because it interferes with the development of an immune system that makes affected birds more vulnerable to variety of infectious. It is very important to detect subclinical infection of CIA in flock as it is a risk factor for a clinical outbreak in the next flock and it can also cause immunosuppression which can lead to substantial economic loss. The present study was carried out in broiler birds belonging to 25 commercial farms suspected for CIA. The broiler birds having 10 per cent or above variation in size was selected. The pathological study comprised of information related to age, clinical signs, mortality pattern, hematobiochemical studies and gross as well as microscopic lesions in organs like thymus, bursa, bone marrow, liver, spleen and lung of affected broilers. The molecular study comprised extraction of viral DNA from different tissue by PCR for detection of CAV and also for phoA gene of Escherichia coli.ThesisItem Open Access PATHOLOGICAL STUDIES ON ESCHERICHIA COLI INFECTION IN BROILERS WITH SPECIAL REFERENCE TO ANTIBIOTIC RESISTANCE GENES(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) Sagar R. Surjagade; Dr. B. P. JoshiThe present study was carried out to know the incidence, mortality pattern, gross and microscopic lesions of the E. coli infection in broiler chicken as well as to know the antibiogram profile and antibiotic resistance gene in E. coli. The study comprised of epidemiological information in relation to autopsy incidence and farm wise mortality, gross and histopathological examination of different organs, isolation, identification, biochemical characterization and molecular detection of E. coli. Further antibiogram profile and detection of antibiotic resistance genes of E. coli isolates were carried out.ThesisItem Open Access STUDIES ON PREVALENCE OF SUBCLINICAL COCCIDIOSIS AND NECROTIC ENTERITIS IN BROILERS(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) Prajapati Ramdatt K.; Dr. D. J. GhodasaraThe present study was carried out to know the prevalence of subclinical coccidiosis and necrotic enteritis in broilers affected with different diseases / pathological conditions received for post-mortem examination. The study was carried out in 50 broiler flocks of 2-7 weeks age. The study comprised of direct smear examination, molecular detection and oocyst count (OPG) of subclinical coccidiosis and molecular detection of Clostridium perfringens.ThesisItem Open Access PATHOLOGICAL AND IMMUNOLOGICAL STUDIES ON IMMUNE COMPLEX VACCINE OF INFECTIOUS BURSAL DISEASE IN BROILER CHICKS(Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) Kapadiya Bhumikabahen; Dr. B. P. JoshiThe present study was carried out to know the protective efficacy of immune complex vaccine in comparison to commercially used intermediate plus vaccine in broiler chicks by experimental challenge with field IBD virus. The study comprised of epidemiological information in relation to autopsy incidence, pathological studies like clinical signs and mortality, gross and histopathological lesions in bursa of Fabricius (bursal score), bursa of Fabricius : body weight (BF : BW) and immunological studies like estimation of IBD and ND antibody titers were carried out. Further detection of IBD virus from bursa of Fabricius was also carried out.
