PATHO - EPIDEMIOLOGICAL AND MOLECULAR IDENTIFICATION OF CHICKEN ANAEMIA VIRUS IN BROILERS
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Date
2020
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Department of Veterinary Pathology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand
Abstract
Chicken infectious anemia (CIA) is known to be an emerging, immunosuppressive and highly contagious disease that affects young broiler chickens from 3 weeks of age. The disease's causative agent is Chicken anaemia virus (CAV), which belongs to the genus Gyrovirus of Circoviridae family. In commercial broilers, there is a better need to diagnose the sub-clinical type of CIA in the field because it interferes with the development of an immune system that makes affected birds more vulnerable to variety of infectious. It is very important to detect subclinical infection of CIA in flock as it is a risk factor for a clinical outbreak in the next flock and it can also cause immunosuppression which can lead to substantial economic loss.
The present study was carried out in broiler birds belonging to 25 commercial farms suspected for CIA. The broiler birds having 10 per cent or above variation in size was selected. The pathological study comprised of information related to age, clinical signs, mortality pattern, hematobiochemical studies and gross as well as microscopic lesions in organs like thymus, bursa, bone marrow, liver, spleen and lung of affected broilers. The molecular study comprised extraction of viral DNA from different tissue by PCR for detection of CAV and also for phoA gene of Escherichia coli.