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ThesisItem Open Access STUDY ON GENITAL INFECTIONS IN POSTPARTUM AND REPEAT BREEDER CROSSBRED CATTLE BY RAVAL SAURABHKUMAR RAMESHCHANDRA (B.V.Sc. & A.H.) (Registration(DEPARTMENT OF GYNAECOLOGY AND OBSTETRICS COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) RAVAL SAURABHKUMAR RAMESHCHANDRA; Dr. M.T. PANCHALThe present study was conducted on postpartum (PP, n=30) and repeat breeder (RB, n=40) crossbred (CB) cows, presented at AI Centres of Chikhodra and Bedwa villages of Anand district, during August 2016 to February 2017, to evaluate genital infections. Based on bacterial isolates, the animals were subgrouped, which comprised Group I (PPT: PP Treatment, 19); Group II (PPC: PP Control, 11); Group III (RBT: RB Treatment, 28); and Group IV (RBC: RB Control, 12). The cows yielding bacterial growth were treated with sensitive antibiotics (i/m) and cows with no bacterial growth (control) were given 10 ml of normal saline (i/m) and were inseminated and followed for repeating to oestrus and/or pregnancy. The details of bacterial isolates, their antibiogram, PMN%, Whiteside test, pH, spinnbarkeit, inter estrus intervals and fertility results were recorded and analyzed.ThesisItem Open Access EVALUATION OF DOUBLESYNCH AND PRID + PMSG FOR OESTRUS SYNCHRONIZATION IN POSTPARTUM ANOESTRUS BUFFALOES(DEPARTMENT OF ANIMAL REPRODUCTION GYNAECOLOGY AND OBSTETRICS COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Patel Arpit J.; Dr. J.A. PatelThis investigation was aimed to evaluate the clinical response and monitor the peripheral plasma progesterone and biochemical profiles in postpartum true anoestrus buffaloes treated with different hormonal protocols, viz., Doublesynch, Estradoublesynch, Triu-B/PRID, PRID + PMSG and untreated control, n=11 each under field conditions. All anoestrus animals identified were initially dewormed using Inj. Ivectin, 10 ml s/c (100 mg Ivermectin), and were treated once initially with Inj. Tonophosphan, 10 ml and Inj. Intavita- H,10 ml i/m (Vit.AD3E) and bolus Minotas @ 1 bolus daily PO for 7 daysThesisItem Open Access COMPARATIVE EFFICACY OF VARIOUS PROTOCOLS OF ESTRUS SYNCHRONIZATION IN CYCLIC AND ACYCLIC BUFFALOES(DEPARTMENT OF ANIMAL REPRODUCTION GYNAECOLOGY AND OBSTETRICS COLLEGE OF VETERINARY SCIENCE & ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Jignesh P. Prajapati; Dr. D.M. PatelThis study was undertaken in field conditions during the year 2016-17 on 50 acyclic/anestrus and 50 cyclic repeat breeder buffaloes to evaluate the efficacy of four estrus induction/synchronization protocols, viz., Doublesynch, Estradoublesynch, Ovsynch, and Ovsynch Plus (10 buffaloes in each protocol, and in untreated cyclic and acyclic controls) in terms of estrus induction response, conception rates at induced estrus with fixed time AI (FTAI) and overall of 3 cycles, including monitoring plasma progesterone, protein and cholesterol profile at different time intervals during treatment and day 12 post-AI. All the animals selected were initially injected with 100 mg Inj. of Ivermectin s/c and Inj. inorganic phosphorus (Inj. Tonophosphan) and multivitamins AD3E (Inj. Intavita-H) 10 ml each i/m and bolus Minotas 1 bolus daily orally for 7 days. Repeat breeding buffaloes received additional single shot i/m injection of Enrofloxacin (Inj. Flobac SA, 40 ml) to rule out invisible infection, if any.ThesisItem Open Access EVALUATION OF ANIMAL PROTEIN FREE SEMEN EXTENDERS FOR CRYOPRESERVATION OF CATTLE AND BUFFALO SEMEN(DEPARTMENT OF ANIMAL REPRODUCTION GYNAECOLOGY AND OBSTETRICS COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Chaudhary Parth J.; Dr. A.J. DhamiThis investigation was undertaken during the favourable breeding season of the year 2016-17 on three mature Gir cattle and three Surti buffalo bulls at Central Sperm Station of the College. The study covered evaluation of seminal characteristics in neat semen and then comparative efficacy of egg yolk based standard TFYG (Tris-citric acid-fructose-egg yolk-glycerol) extender and soybean based commercially available extenders (Optixcell®, IMV, France, and Andromed®, Minitube, Germany) under split-sample technique through various morphological and functional attributes of spermatozoa including leakage of spermatozoa enzymes GOT-GPT in the semen extended/processed in these extenders for cryopreservation, and interrelationships of sperm quality parameters of fresh, pre-freeze and post-thawed semen.ThesisItem Open Access STUDIES ON PHYSICAL CHARACTERS AND PRESERVATION OF SURTI BUCK SEMEN UNDER REFRIGERATION AND DEEP FREEZING AND THEIR FERTILITY TRIALS(AAU, Anand, 1989) Deshpande, Satish Balkrishna; Mehta, V. M.In the present studies on Surti buck semen (i) Physical characters (ii) Effect of dilutors viz., Egg Yolk Citrate (EYC), Tris Egg Yolk Citric Acid Fructose (TEYCAF) and Goat Milk and dilution rates (1:5, 1:10, 1:20 and 1:40) on sperm motility and live sperm count at different hours of preservation (0, 12, 24, 48, 72 and 96) and (iii) effect of dilutors i.e. Egg Yolk Citrate Fructose Glycerol (EYCFG), Tris Egg Yolk Citric Acid Fructose Glycerol (TEYCAFG) and Goat milk glycerol (Goat-milk G) with three different levels (4, 5 or 6 per cent) of glycerol on pre-freeze (P1 and P2) and post-freeze (P3 and P4) sperm motility and live sperm count were studied.ThesisItem Open Access ASCORBIC ACID AND CHOLESTEROL IN THE ADRENAL GLANDS OF INTRA UTERINE CONTRACEPTIVE DEVICE INSERTED SURTI BUFFALO HEIFERS(AAU, Anand, 1970) ZALA, P. M.; MEMON, G. N.Abstract not AvailableThesisItem Open Access VARIATIONS IN THE GONADOTROPHIC HORMONE LEVELS IN THE ANTERIOR PITUITARY GLAND, DURING THE OESTROUS CYCLE IN BUFFALO(AAU, Anand, 1970) Agarwal, Shanti Prasad; Buch, N. C.Abstract not AvailableThesisItem Open Access STUDIES ON FACTORS AFFECTING ANOESTROUS (pubertal and Postpartum) STATE IN GIR CATTLE AND TRIALS FOR RESUMPTION OF OVARIAN FUNCTION(AAU, Anand, 1980) KODAGALI, SHRINIVAS BINDURAO; Deshpande, B. R.Abstract not AvailableThesisItem Open Access REPRODUCTION IN MARES WITH SPECIAL REFERENCE TO ENDOMETRIAL BIOPSY(AAU, Anand, 2012) KUMAR, NISHANT; Patel, D. M.The present study on "Reproduction in Mares with Special Reference to Endometrial Biopsy" was carried out on mares of different police head quarters like Anand and Kheda districts. The mare visiting Teaching Veterinary Clinical Service Complex, College of Veterinary Science and A.H., Anand, were also included in the study. The research work included study of normal and abnormal estrous cycle of mare. In this study special emphasis was given on collection of endometrial biopsy for histopathology and blood for different hemato-biochemical parameters. A detailed histopathological study was carried out on endometrial biopsies to know the reproductive status of mares. The research work was carried out on 18 police mares and privately owned mares of Anand and near by Anand area. Each of the three groups had 6 animals and were divided on the basis of reproductive status, viz.. Group 1- Mares with normal estrous cycle, Group 2- Mares with abnormal estrous cycle. Group 3- Mares with infertility. These mares were studied for normal and abnormal estrous cycle, histopathology of endometrial biopsy and hemato-biochemical studies. On the basis of study in 172 mares it was found that mare is a seasonal polyestrous animal with breeding seasons in the months of spring and summer. As day length increases in spring mares show signs of heat and as day length decreases in winter mare were found to be going into deep anestrous condition. The mean estrous cycle length of mares during breeding seasons were found to be 21 ±0.58 days with estrus period of 5-7 (6.07 ± 0.87) days. The signs of estrus include frequent urination, deviating tail away from the perineum, standing still with the hind limbs spread apart, clitoral winking (rhythmic eversion of the clitoris), squealing, kicking and sensitivity over the flanks, hindquarter, and abdomen. The recently parturited mares were showing foal heat between 7-13 (9.38 ± 2.57) days of parturition. The mean estrous cycle lengths of abnormal cyclic mares was 10 ± 2.32 days (Short) and 32 ± 3.6 days (Long) during breeding season of spring and summer months. The abnormal cyclic mare were also shown wide range of estrus period of 3- 10 (5.6 ±2.8) days. Endometrial biopsy and histopathology studies indicated normal endometrium exhibiting mild to moderate neutrophil infiltration, specially in case of estrus period. In estrus the epithelial cells were tall cuboidal to low columnar and progressing to high columnar during diestrus. Acute endometritis was characterized by accumulation of inflammatory cells (neutrophils), mild to moderates stromal fibrosis, stromal oedema, congestion of blood vessels and accumulation of inflammatory exudates which may be temporary in nature. Chronic endometritis was characterized by moderate to severe endometrial gland atrophy, extensive fibrosis (i.e., more than five layers of fibrocytes around endometrial glands) and mononuclear cell (specially lymphocytes) infiltration. In some cases diffiisely less density of endometrial glands were found The serum calcium levels of normal cyclic, abnormal cyclic and infertile mares were 12.09 ± 0.14, 11.04 ± 0.15 and 10.62 ± 0.10 mg/dl, respectively and was found to differ significantly to each other. The serum magnesium levels of normal cyclic, abnormal cyclic and infertile mares were 2.13 ± 0.06, 1.96 ± 0.03 and 1.85 ± 0.03 mg/dl. The serum phosphorus levels of normal cyclic, abnormal cyclic and infertile mares were 3.86 ± 0.07, 3.22 ± 0.10 and 2.63 ± 0.11 mg/dl. Both serum magnesium and serum phosphorus levels were significantly lower in infertile than normal and abnormal cyclic animals.