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ThesisItem Open Access Studies on certain gastro-intestinal nematodes with special reference to those found in goats(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Sathianesan, V; KAU; Kalyanasundaram, RLife history of Triohuris globuloss has been elucidated for the fist time using kids !q as experimental animals. Both free living and parasitic stages have been described in detail with illustrations. The prepatent period of Triohuric globuloss was found to be 85-89 days. T.globulosa failed to develop in guniea pig on experimental transmission. Haematology and histopathology of trichuriacis in kids experimentally infected with T.globulosa have been studied for the first time and heavy infection with the worm was found to cause anaemia and pathology changes in cacum of the host. Prepatent period of Strongyloides napillosus in kids infected orally with 7000 larva has been determined for the first time to be 6-7 days. Neoasoais vitulorum of calves has been found to be not transferable to oat either transplacentally or orally. In gunia pigs M.Vitulorum failed to attain sexual maturity. Calves could not be infected with infected with M.vitulorum post-nataly. Oesphagostomum colubianum of goats has been found to be not transferable to calves. Prepatent period of O.colusbionum in kids infected experimentally was found to be 40 days. Comparative efficacy of 4 anthelminties viz., Oxibendazole, thiophanate, Albendazole and Parbendazole was increased by conducting treatment trials. Against monospecific infection of Trichuris globulosa in experimentally infected kids the efficacies of Oxibendazile, thiophanate, Albendazole and Parbendazole at the recommended doses of 10mg.50mg, 10mg and 30 mg/kg body weight respectively were found to be 48.6%, 41%,47.3% and 49.3% respectively. Even at double the recommended doses the anthelminations were found to be well tolerated by the kids. Oxibendazole and Albendazole at the rate of 10mg and 10mg/kg body weight respectively were found to be 100% found to be 100 % effective against colubriformise, effective against respectively were Haemonohus contortue, trichostrongylus .Thiopanate at the recommended dosage (59mg/kg body weight) was also 100% effective against all he nematodes mentioned above except Haemonous contortus against which it was only 97% effective at that dosage. Parbendazole at the recommended dosage shedule (30mg/kg body weight) was 100% effective against the nematodes with the exception of .against which only 86.6% efficacy was noticed. Against in nature nematodes in the gastro-intestinal tract Oxibendazole and Albendazole were 100% effective whereas the efficacies of P`arbendazole and thiophanate were 85% and 74% respectively at their recommended doses. In in vitro studies the anthelmintic efficacy of Oxibendasole against Haemonchus contortus was found to be superior to that of the remaining anthelminties. In ovicidal property also, Oxibendazole was better than all the others, since it was ovicidal in 6 hours after administration whereas albendazole, thiophanate and Parbendazole were ovicidal in 8 hours, 9 hour and 11 hours respectively. Albendazole was found to be superior to al the other 3 anthelmintion in their influences on the body weight gain of treated animals closely followed by Oxibendazole. Perbendazole and thiophanate were inferior to others Judging on the basis of over all efficacies Oxibendazole and Albendazole were found to be superior to thiophanate and Parbendasole. Treatment trials with Oxibendazole, Albendazole and thiophanate against gastro-intestinal nematodes of goats have not been attempted earlier than the present study.ThesisItem Open Access Postnatal development of testis and epididymis, semen characteristics and fertility of brown- swiss crossbred bulls(Department of Animal Reproduction and Gyneacology, College of Veterinary and Animal Sciences, Mannuthy, 1981) Surendran Varma Raja, C K; KAU; Ramachandran Rao, AA systematic investigation involving the use of 24 Brown Swiss crossbred bull calves and 7 adult bulls with 62.5% exotic inheritance varying in age from 0- 360 days and 22-40 months respectively, resred and maintained at Indo-Swiss Project, Mattupatty, Kerala, was carried out in order to (a) trace the postnatal growth and development of the testis and epididymis and (b) assess the semen characteristics such as volume, initial motility, sperm concentration, live sperm count and incidence of abnormal sperms; As a corrolary to and as an integral part of this comprehensive study, 6679 semen collection particulars from the records maintained at the Indo-Swiss Project, Mattupatty and 7586 insemination data at the Crossbreeding Research Centre, Muvathuphza werecritically scrutinised to deduce freezability/discard rate of the ejaculates and the fertility potenoy of the bulls, respectively. The materials used, the salient observations made and the valid inferences drawn aare given below: Twenty four pairs of testis and epididymis collected from the bull calves were processed to study the postnatal growth and development. The pattern of growth of testis and of the epididymis was found to be curvilinear. The testicularand epididymal weights were observed to be significantly (p<0.01) correlated with the age, and body weight of the animals. A highly significant (p<0.01) correlation between epididymal weight and testicular weight was also observed. The growth rate of the seminiferous tubules appeared to be of sigmoid type. Formation of lumen in the seminiferous cords was initiated at 150 days and completed, at 180 days of age. The transformation of gonocytes to spermatogonia occurred at 150 days of age. The formation of primary spermatocytes, spermatid and spermatozoa cocurred at the age of 150 days, 300 days and 300-360 days, respectively. The process of transformation of supporting cells was initiated at 120 days and completed at 180 days of age. The transformation of immature Leydig cells to mature cells was initiated at 150 days and completed, by 180 days of age. There were distinct regional differences between the caput, the corpus and the cauda epididymis in respect of the diameter of the tubules, height of the epithelium and the degree of transformation of Epithelial lining from simple columnar to pseudostratified type. The distal part of the ductus epididymis seemed to have attained the adult size at an earlier age than the proximal. Sperms were seen in all the three regions at about 300 days. The mean values of ejaculate volume, initial motility, sperm concentration, live sperm count, sperm head abnormalities, tail abnormalities and proximal protoplasmic droplets were found to be 2.65 ± 0.84 ml, 66.24 ± 0.87%, 1599.64 ± 55.371 milions per ml of semen, 72.55 ± 1.310%, 4.91 ± 0.439%, 0.65 ± 0.045%, 6.47 ± 0.775% and 1.20 ± 0.143%, respectively. Significant (p<0.01) variations between bulls were observed in respect of sperm head abnormalities, tail abnormalities and proximal protoplasmic droplets and between months, in respect of ejaculate volume, sperm head abnormalities, free loose head, tail abnormalities and proximal protoplasmic droplets. Seasonal variation (p<0.05), however, was evident only in the incidence of tail abnormalities. Variations due to age of bulls were found to be significant (p<0.01) in the casse of ejaculate volume, sperm concentration, free loose head, middle piece abnormalities and tail abnormalities. The freezability of sperms expressed in terms of per cent of post-thawing motility was found to be 36.86. There was no seasonal effect on the freezability of sperms. The freezability rate increased significantly (p<0.01) with advancement of age of bulls. The overall rejection rate of the ejaculates was noted to be 39.80%. Most of the rejection (71.75%) was done before freezing. The pre-freezing rejection was found to be 28.55% of the total ejaculate. The post-freezing discard to be 15.74% of the frozen samples. The rejection rates of the ejaculates differed significantly (p<0.01) between months/seasons/age of the bulls. The rate of rejection was found to decrease with advancing age. The overall fertility rate of the bulls was found to be 56.06%. There were significant (p<0.01) differences in the fertility rate between months/seasons of insemination and between age of the bulls. As between bulls also, there was a significant variation (p<0.01) in the fertility rate. The significance of the observations inferences indicated above and their relevance to the implementation of cross breeding programme in Kerala were discussed briefly.ThesisItem Open Access Studies on the common ticks affecting live-stock in Kerala(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Rajamohan, K; KAU; Kalyanasundaram, RAn investigation was conducted for a period of 5 years from 1974 to 1979 in to the incidence of ticks on domestic animals in Kerala, the biology of the most common species, and their susceptibility to four commonly available organo phosphorus compounds.. Nine different species of ticks belonging to 5 genera were collected from cattle, buffaloes, goats and dogs in different localities of the eleven districts of Kerala. The species of ticks identified were boophilus annulatus, rhipicephalus sanguineus, R.haemaphusaloides, R.turanicus, haemaphysalis bispinosa, H. turturis, H.spinigera, Hyalomma anatolicum and Amblyomma integrum. The commonest among them was boophilus annulatus, which was found mainly on cattle. The incidence of this tick was very high in the hilly areas and in the western ghat regions. The other common ticks found on cattle were Rhipicephalus haemaphysaloides and Haemaphysalis bispinosa. Tick infestation was found to be rare in buffaloes and goats and the common species found on them were Haemaphysalis bispinosa and B. annulatus. IN dogs the species recorded in the present study were Rhipicephalus sanguienus and R. turanicus. Variations in incidence was evidenced from the present study. The maximum incidence was found in the months of July, August, September, October and November. The life-cycle of B.annulatus was completed experimentally in a period 44 days. The maximum number of eggs produced by one tick was 1420 and minimum 680. The incubation period was dependent on the room temperature and variation in humidity had little influence. The larvae were found to prefer hairy areas of the body for attachment, and they did not attach at the hairless and exposed areas. The engorged females were found to drop off quickly when green grass was spread on the floor of the experimental animal’s shed. The life-cycle patter could be summarised as: Pre-oviposition : 1 to 6 days Oviposition : 5 to 9 days Incubation period : 16 to 30 days Larval fasting : 2 to 5 days Larval feeding : 4 to 7 days Larval moulting : 8th to 12th day of infection Nymphal feeding : 3 to 6 days Moulting of Nymph : 15 to 19th day of infection Adult feeding : 5 to 10 days Detatchment of female : From 20th day of infection onwards Four organophosphorous compounds viz. Malathion, Fenitrothion, Dichlorvos and Phosalone were tested for their action on ticks. The ovicidal and larvicidal actions and the lethal effects on nymphas and engorged females of B. annulatus, R sanguinius and H. bispinosa were studied in the laboratory. Cythion (malathion 50%) 0.5 to 1%, Sumithion (Fenitrothion 50 %) 0.5 to 1%, Dichlorvos (Nuvan) 0.1 to 0.2%, and Zolone (Phosalone 35 %) 0.2 to 0.3% were found effective at different degrees. The efficacy was directly proportional to the concentration of compound and the duration of treatment. Phosalone showed maximum efficacy followed by Dichlorvos. In field trials, all the four compounds gave satisfactory results. But phosalone showed maximum efficacy and least toxicity to animals. Nuvan was also effective but was the most toxic among the four compounds. Thus the acaricide of choice for routine use in Kerala was determined to be Phosalone (35%) at a concentration of 0.3% to be used as a spray or wash.ThesisItem Open Access Influence of particle size of feed and plane of feeding on growth,physiological reactions and carcass characteristics of growing-finishing pigs(Department of Livestock and Production Management, College of Veterinary and Animal sciences, Hissar, 1981) Kurien, Thomas; KAU; Singh, R AThirtysix Large White Yorkshire barrows averaging 70 days of age and 11.2 kg weight were assigned at random to six dietary treatments in order to study the influence of feed particle size and level of digestible energy on the performance, physiological reactions and carcass characteristics. They were individually housed and maintained on diets compounded mainly of dried sardines, dried tapioca, groundnut cake and rice bran till they were 8 months of age. The barrows were provided with the experimental diets having 100%, 90% or 85% of NRC standards of DE with feed particle size of less than one or two to three millimeter. It was observed that variations in feed particle size did not produce any significant effect on growth, physiological reactions and carcass characteristics of pigs under any of the treatments. At 8 months of age, the pigs under treatments 1 and 4 (100% DE of NRC; 2 and 5 (90% DE of NRC) and 3 and 6 (85% DE of NRC) had, (i) a final weight of 102.9; 99.4; 86.8; 92.3; 67.1 and 67.2; (ii) an average daily gain of 0.545; 0.524; 0.448; 0.473; 0.329 and 0.326 kg, and (iii) feed conversion ratio of 3.4; 3.5; 4.3; 4.2; 5.6 and 5.4, respectively. The pigs under treatments 1 and 4 reached 70 kg weight at an age of 197.5 days on an average, while pigs under treatments 2 and 5 reached the same weight by 208.5 days, and under 3 and 6 by 228 days. The digestibility of dry matter, organic matter, ether extract and crude carbohydrate in pigs under all the treatments was significantly (p <0.01) lower with decreasing levels of DE at 5 and 7 months of age. Although the digestibility of crude protein was significantly higher (p <0.01) in pigs that received 100% DE of NRC than 85% at 5 and 7 months of age, it was statistically of the same order with that of 90% at 5 months of age. Dietary treatments had no significant effect on the rectal temperature, heart rate, respiration rate and haemoglobin concentration of blood of the experimental pigs. The yield of dressed carcass was decreased from 72.3% to 68.8% or 64.7% with the decreasing body weight of pigs on 100%, 90% or 85% DE of NRC standards, respectively. The yield of ham was increased from 21.3% to 22.5% or 23.5% with the decreasing levels of DE, respectively. The length of carcass, eye muscle area and overall backfat thickness were significantly (p <0.01) lower as the level of DE was decreased. The fat percentage in dried eye muscle was almost uniform (22.3 to 24.9%) in pigs under different dietary treatments. The quality score for marbling, colour and firmness was significantly (p <0.01) in favour of diets that contained higher of DE. The feed cost per kg body weight gain of pigs under different dietary treatments or total cost of feed and labour at market weight of 70, 80 and 90 kg were determined. The cost of raising pigs from weaning to these weights on rations containing 90% DE of NRC standards and two to three millimeter feed particle size was found to be most economic. An equation W = 5.16 + LG2/11568 was evolved predicting live weight of barrows ranging from 8 to 144 kg, where W, is the weight in kg, L, is the length of body in cm, and G, is the girth in cm.ThesisItem Open Access Studies on heterosis and combining abilities with respect to important economic traits in Capsicum annuum, L.(Division of Agricultural Botany, College of Agriculture, Vellayani, 1981) Manikantan Nair, P; KAU; Mary, K GeorgeSixty three varieties of Capsicum annuum representing different agroclimatic regions of the country constituted the base material of the study. Based on yield potential , adaptability and tolerance to leaf curl complex disease, thirty varieties were selected for subsequent studies. Comprehensive genetic studies including estimation of genetic parameters, cause effect relationship by path coefficient analysis, genetic divergence by Mahalanoble D2 statistic and a nine parent diallel analysis to causes the combining ability, to unravel the pattern of inheritance and also to examine the level of heterosis manifested with respect to eighteen economic characters including four nutritiveand quality attributes namely Vitamin A, Vitamin C, Capsaicin and Oleoresin content were conducted during the year 1976-79. Analysis of plot means exhibited highly significant differences among varieties except for Capsaicin content. The high degres of variability in economic attributes offers scope for recombining desirable genes from different varieties.ThesisItem Open Access Pedologic studies on lateritic catenary sequences occuring in Kerala(Department of Soil Science and Agricultural Chemistry, College of Agriculture, Vellayani, 1980) Venugopal, V K; KAU; Koshy, M NThree toposequences were identified at Varkala, Poruvazhy and Kalliaserry in the midland laterite regions of Kerala using soil maps prepared by the Soil Survey Wing of the Department of Agriculture. A systematic study of these catenary sequences along transects extending from crest to valley was made. Profile pits were dug at the different physiographic positions of the transects, viz., crest, upper midslope, nearly level lower slope and valley at each of these sites are examined for their morphological features. Soil samples representing the different horizons were also collected for laboratory studies. The physicochemical charecteristics, mineral assemblage of fine sand fraction and the minerology of the clays in the profile samples were studied with a view to relating these properties to the genesis of the four diverse groups of soils formed in the transects . Patterns of similarity or otherwise between the three locations of widely different conditions and the four physiographic positions were brought out .ThesisItem Open Access Studies on the physiological and biochemical changes in relation to reproductive efficiency in Chickpea (Cicer arietinum L.)(Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 1981) Seshadrinath, S; KAU; Sabra AbbasThe average yield of chickpea in India is as low as 700 kg/ha. One of the major causes for this low yield can be attributed to the low reproductive efficiency. The cause for low reproductive efficiency is mainly due to heavy shedding of reproductive structures. The present investigations were, therefore, initiated with the following objectives: 1) To study the genetic variability and diversity with reference to the flowering behaviour and shedding of flowers during the reproductive stage in order to evaluate the peak periods of flowering, shedding percentage and reproductive efficiency.ThesisItem Open Access Use of insecticides as granules for protecting paddy crop against pests(Division of Agricultural Entomology, College of Agriculture, Vellayani, 1981) Sasidharan, Pillai K; KAU; Gopalakrishnan, Nair N R
