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Cryosurgical treatment for experimentally induced cataract in dogs 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
(Department of Surgery, College of Veterinary and Animal Sciences,Mannuthy, 2000) Neelakanta Praveen, Pillai; KAU; Ravindran Nair, S
This study was conducted with the objective of comparing the efficacy of cryo-coagulation and intra-capsular cryo-extraction of experimentally induced cataract in dogs. Twelve nondescript dogs aged approximately one year were used for the study in two groups, A and B, each consisting of six animals. Two clinical cases of cataract were included under the study as Group C. In all the animals of Group A and B, cataract was experimentally induced by injection of 0.5 ml of a 25% solution of calcium borogluconate into the anterior chamber of the eye, in strict aseptic conditionsand under general anaesthesia. In group A. cataract was treated by cryo-coagulation and in Groups Band C intra-capsular cryo-extraction of the cataractous lens was performed after pre-medicating and anaesthetising the animals. Surgery for cryo-coagulation (Group A) or intra-capsular cryo-extraction (Groups B and C) was performed under general anaesthesia with thiopentone sodium after premedication with xylazinc hydrochloride. The anaesthesia was found to be satisfactory at the time of induction and during cataract surgery. Extensive lateral canthotomy was found to be necessary in all the animals at surgery in order to ensure adequate exposure of the globe. Rectal temperature and respiration rate dropped slightly post-operatively, but returned to normal values by the second day after surgery. Pulse rate dropped markedly following surgery, but returned to normal by the sixth day after surgery. Total leukocyte count increased slightly 24 hours after surgery. but then decreased and remained within normal ranges thereafter. There was increase in neutrophil. eosinophil and monocyte count had increased upto 24 hours after surgery. but was normal thereafter. Lymphocyte eount decreased upto 24 hours post-operatively. All the animals .remained in good condition throughout the observation period, except for one animal. Conjunctivitis persisted only in one upto the 11 th day. Corneal oedema persisted throughout the period of observation in four animals. One animal had complete corneal clarity by day seven. In the other animal the cornea cleared on day 23. Uveitis persisted for varymg periods in the animals Photophobia and blepharospasm resolved by day six in all animals. One animal had no posterior or anterior synechiae following surgery and its vitreous body was clear, allowing easy exam ination of the retina. Aqueous flare, indicative of increased protein in the aqueous humour, could not be determined in any animal. Functional vision was not returned in any animal except A4. In Group B, vitreous prolapse occurred during surgical removal of the lens by intra-capsular cryo-extraction. This prolapsed vitreous was excised and did not cause complications. Rectal temperature increased slightly 24 hours after surgery, but had returned to normal values by the 15th post-operative day. Pulse rate decreased slightly 24 hours after surgery, but attained normal values by the second post-operative day and stayed so thereafter. Respiration rate decreased markedly for 24 hours following surgery, but reached normal values two days post-operatively. There was no change in the colour of the mucous membrane of the contralateral eye (used as control) at any time during the period of : observation. The total leukocyte count increased slightly following surgery and continued to be so till 24 hours after surgery and thereafter it decreased and was within normal limits thereafter. The neutrophil count increased till 24 hours post-operatively and returned to normal range thereafter. Lymphocyte count decreased slightly 24 hours after surgery, but returned to normal range by the i s" post-operative day. Eosinophil count increased 24 hours after surgery, but had reached normal values by the i s" post-operative day. Monocyte count became zero after surgery, but then increased slightly and continued so thereafter. All the animals remained in good general condition until the end of the observation period, with no evidence of infection in the operated eye. Intra-ocular pressure decreased slightly following surgery, but had returned to normal ranges by the end of the observation period. Animals B 1, B2 and B4 had persistent conjunctivitis and corneal oedema throughout the period of observation and were unable to negotiate an obstacle course or locate and track mobile or stationary objects even in conditions ofbright ambient light. All other animals in this group were able to perform satisfactorily in the tests of visual function by the end of the observation period. In Group C, treatment of cataract was by intra-capsular cryo- extraction, as in Group B. The results obtained were similar to those for Group B. Animal C2, however. took 52 hours to recover from anaesthesia and died on the sixth day following surgery. The death could not be attributed to complications of cataract surgery. Rectal temperature decreased slightly following surgery, and then increased slightly but returned to normal values at the end of the observation period. Pulse rate decreased immediately after surgery and then returned to normal ranges by the eighth day after surgery. Respiration rate decreased markedly immediately after surgery. but returned to the normal range within 24 hours after surgery. Colour of mucous membrane of the contralateral eye did not show any change at any time during the period of observation. Total leukocytc count increased slightly upto 24 hours after surgery but returned to normal ranges thereafter. The neutrophil and eosinophil and monocyte counts increased after surgery but returned to normal ranges thereafter. The lymphocyte count was markedly decreased at 24 hours after surgery, but then returned to normal ranges thereafter. The surviving animal in Group C showed low grade corneal oedema until day 31 following surgery, but it had blink reflexes and the iris was visible. Conjunctivitis had cleared by day six following surgery. The animals were monitored for visual capability following surgery. The tests were conducted by evaluating the animals' ability to negotiate an obstacle course under photopic and scotopic light conditions, after blind folding the left eye with an eye shield. The animals were also tested for their ability to locate a stationary object and to track a moving object under varying conditions of ambient lighting. Tests of ocular functional integrity were conducted by evaluating menace and photomotor pupillary reflexes. Animal Cl was able to locate or track stationary objects In dim light. It could track moving ohjects in all light conditions. From the results obtained in thc present study. it was found that only one out of the six animals showed restoration of functional vision following treatment of cataract by cryo-coagulation of the lens. In the case of the treatment by intra-capsular cryo-extraction. four out of eight animals showed restoration of functional vision thus showing a success rate of 50% for intra-capsular cryo-extraction of cataract. From the results it can be concluded that: 2. Cataract could be effectively induced using 0.5 ml of calcium borogluconate solution (25%) injected into the anterior chamber of the eye. 3. Pre-medication USing xylazine hydrochloride followed by general anaesthesia USing thiopentone sodium IV was satisfactory for the induction of cataract and the treatment of the cataractous lens. 4. Intra-capsular cryo-extraction IS a better method In treating cataractous lenses in dogs.
Open Access
Ascending coccygeal venography in evaluation of paraplega in dogs
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 2002) Joshi George; KAU; Sarada Amma, T
The study was undertaken with an objective of evaluating the comparative efficacy of ascending coccygeal venography and epidurography in locating the site and type oflesion in dogs suffering from paraplegia. The study was conducted in 12 clinical cases presented at the Surgery unit of Veterinary College Hospital, Mannuthy and were designated into two groups (Group A and B) of 6 animals each. Survey radiographs were taken in all the animals prior to the contrast radiographic procedures. Iohexol (300 mgIfrnl) was used as the contrast medium at a dose rate of 80 mgIlkg body weight for epidurography and 100 mgIlkg body weight for ascending coccygeal venography. In Group A, epidurography was conducted by introducing the contrast medium through the lumbosacral junction into the epidural space. The contrast medium was given as slow infusion and the zero minute radiograph was taken, as the last milliliter of the contrast medium remained to be injected. Subsequent radiographs were taken at the 3rd and the 5th minute. In Group B, ascending coccygeal venography was conducted by introducing the contrast medium intravenously as slow infusion into the superficial lateral coccygeal vein of anyone side near the base of the tail, after sufficient abdominal compression. Lateral/ventrodorsal radiographs were taken, as the last milliliter of the contrast remained to be injected. Subsequent radiographs were taken at the 3rd and the Sth minute. All the animals were subjected to a thorough clinical and neurological examination prior to radiography. Physiological and haematological parameters were evaluatedjust before and 24 hours after radiography. All the animals were observed for one week for the presence of any side effects/complications. The physiological and haematological parameters were within the normal range in all the animals both before and 24 hours after radiography. Iohexol was found safe for neuroradiological studies as none of the animals exhibited any side effects/complications during the period of observation for one week. In Group A, epidurography produced good quality radiographs and was consistent in all the six cases. Abnormalities/lesions could be located in four cases. The remaining two cases showed normal epidurographic pattern and could not be correlated with the neurological examination. In Group B, successful venograms were obtained in three cases in which lesions were located and correlated with the neurological examination. In the remaining three cases, the venous sinuses could not be opacified and hence failed in identification oflesion. The changes identifiable as lesions in Group A were attenuation of the contrast col W11l1 , considerable narrowing and elevation of the column, and failure of the opacification ofa specific region of the column. The changes identifiable with a lesion in venography were abrupt attenuation of the venous sinuses, considerable elevation of the venous sinuses and progressive thinning and attenuation of the veins. Thus epidurography showed consistency III all the SIX cases and diagnosis could be made in four out of six cases whereas diagnostic venograms were obtained only in three out of six cases. Thus ascending coccygeal venography was found inconsistent and required further studies before recommending this as a routine practice. Though epidurography possessed a distinct advan tage over ascending coccygeal venography in consistency and technical and diagnostic feasibility, epidurography couldnot be advocated as a sole diagnostic procedure and should be used as an adjunct with other .diagnostic aids after proper clinical and neurological examination.
Open Access
Processed oesophageal allografts for hernioplasty in pigs
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 2000) Senthil Kumar, S; KAU; Muraleedharan, K N
The present study was conducted with the objectives of: 1. Evaluating the suitability of processed collagen based oesophageal allografts as a biological tissue substitute for hernioplasty in pigs and ii. Comparing the healing in herniorrhaphy and hernioplasty, in pigs. Twelve clinical cases of umbilical hernia in pigs of either sex, aged two to three months were used in the study. The animals were divided into two groups (Group I and Group Il) of six animals each based on the size of hernial ring. Group I animals were subjected to herniorrhaphy and Group Il animals were subjected to hernioplasty. Fresh pieces of oesophagus collected from slaughtered pigs were processed and cross-linked with gl u taraldehyde to prepare the graft. Triflupromazine hydrochloride was administered at the rate of 1.5 mgjkg body weight IjM to all the animals and local infiltration anaesthesia using lignocaine ii hydrochloride at the site was employed for the surgical procedures. In five animals of Group I where the hernial contents were reducible, simple reduction and overlapping mattress sutures with silk were employed for closing the hernial ring. In one animal where it was irreducible, enterotomy was performed to remove the intestinal contents before reduction and herniorrhaphy. In all the six animals of Group II, the hernial ring was large, and the contents were reducible. After reduction, the edges of hernial ring were sutured using silk by simple interrupted sutures, to reduce the size of hernial ring. Processed oesophageal allografts were placed as an only graft over the suture line and fixed using silk sutures. All the animals became active and alert within 24 hours postoperatively except the one, which underwent enterotomy and herniorrhaphy. The surgical site was dressed daily and skin sutures were removed on the seventh postoperative day. In one animal of Group I wound infection resulted in skin wound disruption, which was surgically treated as open wound. Recurrence of hernia was noticed in one animal of Group I, 24 days after herniorrhaphy. Marginal increase in rectal temperature, pulse rate and respiration rate were observed during early Hi postoperative period in all the animals and it became normal within seven days after surgery. Marginal decrease in haemoglobin concentration was noticed during postoperative period in all the animals but it reached near normal level by 21 st postoperative day. Marginal increase in packed cell volume and total leucocyte count was observed up to third postoperative day. Neutrophilia and lymphopenia was noticed during early postsurgical period but reached near normal level by 14th postoperative day. Marginal decrease in total serum protein was noticed during postoperative period but was normal by 21 st postoperative day. Serum sodium and potassium level did not show any significant variation and the changes were within the normal range. Tissue samples were collected from surgical site from three animals each of Group I and Group II on 180 and 195 days respectively for biomechanical studies, gross and histomorphological changes. The tensile strength was greater . in Group II animals than Group I and normal animals. There was no adhesion of viscera at the surgical site. Remnants of silk suture could be identified in all the animals. The histomorphological study indicated complete healing and replacement of the graft material without any untoward effect.
Open Access
Clinical and serum biochemical evaluation in surgery for alimentary tract obstruction in dogs
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 2001) Shejo Jose; KAU; Rajankutty, K
The study was carried out in selected clinical cases of alimentary tract obstructions in twelve dogs of either sex and different breeds, presented to the clinics of the college. These animals were divided into two groups viz. Group I and Group II, each consisting of six animals. Group 1- Dogs with obstructions of upper alimentary tract (oesophagus, stomach, duodenum and jejunum). Group II - Dogs with obstructions of lower alimentary tract (ileum, caecum, colon and rectum). In Group I the obstructions were due to bone pieces, granite stone, cloth piece, partial torsion of stomach and pyloric stenosis. In Group II the obstructions were due to corkscrew, ileocolic intussusception, cloth pieces and faecoliths. The obstructions due to foreign bodies were 50 per cent, with faecoliths were 25 per cent and due to anatomical alterations and malpositions of gastrointestinal tract were 25 per cent. In upper alimentary tract obstruction (Group I), the predominant clinical sign was vomiting. Frequency of vomiting was more than four times a day and vomitus was either frothy or white in colour. Pain on abdominal palpation and constipation were the other symptoms noticed. Respiration rate, systolic and diastolic blood pressure, total leukocyte count, lymphocyte count and blood urea nitrogen, creatinine, total serum protein and serum albumin levels were higher than the normal range, preoperatively. The packed cell volume, total erythrocyte count, haemoglobin concentration and serum sodium, potassium and chloride levels were lower than the normal range, preoperatively. The pulse rate, rectal temperature, neutrophil count, monocyte count and alanine transferase, aspartate transferase levels were within the normal range, preoperatively. In lower alimentary tract obstruction (Group II), constipation was the prominent symptom observed. The faeces was either tarry or blood tinged. The frequency of vomiting was three times or less a day and vomitus was either frothy or yellowish in colour with presence of partially digested food materials. Pain on abdominal palpation was noticed in five animals. The systolic and diastolic blood pressure, neutrophil count and blood urea nitrogen, creatinine, serum albumin, aspartate transferase, alanine transferase levels were higher than the normal range, preoperatively. The packed cell volume, total erythrocyte count, monocyte count and serum sodium, potassium and chloride levels were lower than the normal range preoperatively. The respiration rate, pulse rate, rectal temperature, total leukocyte count, haemoglobin concentration, lymphocyte count and total serum protein level were within the normal range, preoperatively. Plain radiography was found useful for identifying the obstruction in the alimentary tract caused by radio opaque materials. Contrast radiography using barium meal revealed seats of obstructions due to radiolucent foreign bodies and anatomical alterations.
Open Access
Clinical evaluation of xylazine-propofol anaesthesia in dogs
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 2001) Sooryadas, S; KAU; Sarada Amma, T
The study was undertaken to evaluate the anaesthetic effect of propofol in xylazine premedicated canine surgical patients. Fifteen dogs of different breeds of either sex were divided into two groups. Group I consisted of eight apparently healthy dogs presented for elective surgery, and group 11 consisted of seven dogs presented for emergency surgery. All the animals were premedicated with atropine sulphate @ 0.04 mg/kg body weight IM and xylazine @ 1 mg/kg IM. Ten minutes later, general anaesthesia was effected with intravenous injection of 1 % w/v propofol and was maintained with incremental dose(s) ofpropofol as intermittent boli as and when required. Palpebral reflex was sluggish ill both groups during induction and throughout the period of maintenance. Eyeball rolled down during induction and remained in that position throughout the period of maintenance. The duration of anaesthesia after initial bolus was 14.03 ± 2.04 min. in group I and 18.54 ± 1.64 min. in group 11. The average incremental dose of propofol needed was 2.78 ± 0.45 mg/kg in group I and 2.98 ± 0.50 mg/kg in group H. The average duration of effect of incremental dose obtained was 10.04 ± 0.75 min. in group I and 16.85 ± 2.71 min. in group H. Animals of group I were able to stand by 14.72 ± 1.63 min. after recovery while it was 20.9 ± 1.25 min. in group Il. The gait of the animals of group I became apparently normal by 19.69 ± 1.55 minutes, while it was 27.2 ± 3.06 minutes in group II animals. Respiration rate showed significant decrease in both the groups after premedication and at 15 minutes after induction with propofol. Pulse rate and heart rate decreased after premedication and increased on induction with propofol. Electrocardiographic changes were transient. There was decrease in haemoglobin concentration after premedication, which increased on induction in both groups. Total erythrocyte count decreased on premedication and increased on induction. Total leukocyte count decreased after premedication followed by an increase after induction. Neutrophilia was observed both after premedication and on induction with propofol in group I, where as in group II neutrophil count showed a decrease on premedication which increased after induction. Lymphocyte count decreased after premedication and increased after induction in group 1. But in .group II lymphocyte count showed an increase after premedication, which decreased on induction of anaesthesia. Serum sodium and potassium concentrations, total serum protein content and albumin/globulin ratio did not show any significant change during the study .. Propofol under atropine-xylazine premedication was found to be an effective and safe anaesthetic for. induction and maintenance of anaesthesia for surgery in both healthy and compromised dogs with less side effects.
Open Access
Management of tibial fractures in dogs using plaster of paris cast and modified thomas splint
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 2001) Anoop, S; KAU; Balagopalan, T P
The study was undertaken with the objectives of 1. Assessing the healing process based on clinical symptoms, biochemical parameters and radiography of the affected part after immobilization of tibial fractures with plaster of Paris cast and modified Thomas splint. 2. Assessing the effect of electrodiagnostic procedures on healing of fracture of tibia in dogs. The study was conducted in twelve selected clinical cases of fracture of tibia in dogs of either sex presented to the Department of Surgery. These animals were randomly divided into two groups (group 1 and group 11) consisting of six animals each. All the animals were examined clinically and radiographically and observations were recorded. They were premedicated with Atropine sulphate (0.04 mg/kg body weight) intramuscular followed by xylazine hydrochloride (1 mg/kg body weight) intramuscular. The fracture was reduced by closed method in all the animals. The limb was immobilized by application of modified Thomas Splint in Group 1 and by application of plaster of Paris cast in group 11. History and observations on clinical and physiological parameters, haemogram, serum constituents, radiography of the limb and electrodiagnostic procedures were recorded in all the animals on the day of presentation and at the end of first week, second week, third week and sixth week after immobilization. All the animals were kept under observation for a period of six weeks. Though 30 per cent of the animals in each group started bearing weight on the affected limb by the end of first week, fracture instability and other associated symptoms persisted relatively more in animals of group 1. At the end of second week, the symptoms of fractures except oedema were found to be persisting in more number of animals belonging to group 1 due to mutilation of the Thomas splint. By third week all the animals in group 11 could bear weight on the affected limb but limb dysfunction still persisted in fifty per cent of the animals in group 1. By the end of sixth week all the animals in both the groups showed apparently normal use of the limb. Temperature, pulse rate and respiration rate showed variation within normal range during the period of observation in all the animals. Haemoglobin concentration, packed cell volume, total erythrocyte count and lymphocyte count showed marginal increase on first week post fracture followed by a decrease thereafter in all the animals of both the groups. But neutrophil count showed a fall on the first week post fracture followed by a gradual increase in group 1, but in group 11 it revealed a decrease in values throughout the period of observation. Eosinophil and monocyte count showed only marginal variations during the period of observation in both the groups. Serum total protein showed a decrease till second week followed by an increase in group 1 but the variation was marginal in group 11. Serum alkaline phosphatase showed an increase till second week followed by a decrease thereafter in both the groups. Serum aspartate amino transaminase values remained at a higher level in the post fracture period in group 1 but in group 11 it was relatively at a lower level. Periosteal callus started developing near the fracture site along with reduction in soft tissue swelling and opacity of fragment ends in four animals in group 1 and two animals in group 11 by the end of first week. Five animals in group 1 and four animals in group 11 showed presence of massive periosteal callus by the end of third week and three animals in group 11 and two animals in group 1 showed presence of smooth and opaque periosteal callus with complete disappearance of fracture line by the end of sixth week. Eventhough there was marginal increase in the strength required to produce minimal contractile response in the initial period after fracture reduction, by sixth week, the values were near normal at all the duration in both the groups.

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