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  • ThesisItemOpen Access
    Superovulation, Embryo Collection And Cytomorphology of Genital Organs In Prepubertal And Adult Goats
    (Birsa Agricultural University, Ranchi, 1990) Singh Balraj; B.K. Singh
    1.The experiment was conducted on a total of 48 healthy goats comprising of 16 prepubertal and 32 adult parous goats. 2. Prepubertal goats were divided into four groups comprising of four goats in each group. Pregnant sare serum gonadotrophin and human chorionic gonadotrophin either alone or in combination were used to induce superovulation in different groups. 3.Adult goats were divided into six groups comprising of five goats in each group. Two goats were kept as control. Two injections of prostaglandin 72 alpha vere administered intramuscularly in first three groupe and intra vulvo-submucosally in another three groups at 11 days interval. Pregnant mare serum gonadotrophin was administered in each group forty eight hours before the second injection of PG F2 alpha and forty eight hours after the second injection of PG F2 alpha human chorionic gonadotrophin was administered to each goat. 4. The goats were allowed mating with fertile bucks two times (48 and 72 hours post PG ₂ alpha) during heat. 5. Superovulatory response was judged on the basis of numbers of corpora lutea, corpora haemorrhagica and anovulatory follicles on the day of embryo collection. 6.Embryos were collected surgically on day 3 from the oviduota and between day 6 to 8 from the uterine horas. Cytomorphological and cytochemical studies were conducted. 7. Cytomor phological and cytochemical studies were conducted on the genital organs collected from two of the super ovulated goats. 8. Superovulatory response did not increase by increasing the dose of PMSG from 500 IU to 750 IU in prepubertal goats. 9. Ovarian response was significantly higher in right ovary as compared to the left in prepubertal goats. 10. Average number and percentage of ovulations increased significantly (P/ 0.01) with the incorporation of HOG alongwith PMSG and the best results were obtained with 750 IU PASO and 1000 IU HOG in prepubertal goats. 11. Highly significant effect (PL 0.01) of treatments on the number of anovulatory follicles in prepubertal goats was observed, thus, anovulatory follicles were maximum in the group in which highest dose (750 IU) of PMSG alone was administered. 12. A total of 19 embryos (35.18 percent) were recovered from prepubertal goats. Out of 57.89 percent fertilized embryos recovered, 36.84 percent embryos were normal. 13. Prepubertal goats can be superovulated and may be used as embryo donors.
  • ThesisItemOpen Access
    Enzyme Immunoassay of Progesterone In Milk of Bovines During Early Pregnancy And In Serum Of Superovulated Cows
    (Birsa Agricultural University, Ranchi, 1988) Gupta, jSharadendu Kumar; A. K. Sinha
    In super ovulated cows, maximum value of serum progesterone was recorded after PMSG injection, or on the day of second injection of PGF₂ alpha. The super ovulatory response was judged on the basis of palpable corpora lutea on the ovaries upon rectal palpation as good ( 12 corpora lutea), moderate (6-12 corpora lutea) and poor (1-5 corpora lutea). The serum progesterone values were observed to fluctuate consider ably. Whereas, the fluctuations were not so marked in one poorly responding cow.
  • ThesisItemOpen Access
    EVALUATION OF CHILLED AND FROZEN SEMEN FROM CROSS BRED BULLS WITH SPECIAL REFERENCE TO MICROBIAL STATUS
    (Birsa Agricultural University, 1987) Singh, NIMAI; Sinha, A.K.
    The overall mean bacterial load in semen (x 10³/ml) at graded time intervals pre and post freezing with conventional, specific and without antibiotics was 6.88 0.59, 5.38 + 0.16, 3.84 0.22, 2.73 0.14, 2.090.16: 11.56 1.07, 5.84 ± 0-19, 4.51 0.29, 3.32 0.18, 2.50+ 0.16 and 0.347 0.051, 0.273 0.042, 0.224 0.035, 0.163 0.018 and 0.1002 0.007, respectively. Significant effect (p < 0.05) between treatments and between periods of storage was observed.
  • ThesisItemOpen Access
    STUDIES ON GENITALLA OF SOWS
    (Birsa Agricultural University, 1982) DAS, PRAMOD KUMAR; Singh, B.K.
    1.The results obtained in this study was based on examinations of 301 genital organs of female pigs, procured from Government Bacon Factory, Ranchi, during the period from December 81 to July ¹82. 2. Only non-gravid genitalia were used for the study and they belong to four breeds of pigs - Landrace, Yorkahire, crossbred and deshi. 3. Four age groups were made 6 to 12 months, 12 to 18 months, 18 to 24 months and 24 to 48 months and the results obtained in different age group were compared. 4. Observations were made on these aspects biometry of genitalia, abnormalities in pig genitalia and histopathology of same diseases. 5. Two hundred and forty one normal genitalia were used for biometrical study. 6. Sixty (19.94%) genitalia were found to be grossly abnormal. 7. On the basis of palpable structures, left ovary was found to be more functional than the right. 8. Non-significant effect of breed was observed on all the measurements of ovary except thickness. 9. The oviduct of exotic and crossbred pigs was found to be longer than the deshipigo.
  • ThesisItemOpen Access
    STUDIES ON SOME ASPECTS OF REPRODUCTION IN DIFFERENT BREEDS OF PIGS AND RELATIONSHIP BETWEEN REPRODUCTIVE PERFORMANCE AND BLOOD BIOCHEMICAL PROFILE
    (Birsa Agricultural University, 1987) Singh, BIRENDRA KUMAR; Singh, B.K.
    The present investigation was conducted to compare the reproductive performance of three breeds of pigs viz; Large White Yorkshire, Landrace and Hampshire during three seasons. The relationship between reproductive efficiency and blood biochemical profile during different stages of reproduction was also studied. Furthermore, the changes in vaginal epithelium during different stages of oestrous cycle and pregnancy were observed using biopsy technique. (1) The average age at puberty in Large White Yorkshire, Landrace and Hampshire was noted as 320.541.95, 314.68 ± 1.62 and 316.52 1.56 days. The influence of breed on attainment of puberty was significant (PL 0.05). The influence of season on puberty attainment was also significant (FL 0.01) and gilts born during rainy season attained puberty earlier as compared to gilts born during winter and summer season. (2) The mean duration of oestrous cycle and average duration of oestrus did not differ significantly between breeds during different seasons. A significantly (PL 0.01) higher occurrence of oestrus was recorded during winter (42.16± 0.47%) than summer (24.16± 0.51%) and rainy season (33.10 ± 0.49%).
  • ThesisItemOpen Access
    Rovulation and Transcervical collection of ova from repeat breeder cross- bred cows
    (Birsa Agricultural University, 1985) Bhattacharyya, Biswanath; Sinha, A.K.
    A high percentage of ova 66.23 % (51/77) were unfertilized and out of 26 fertilized ova, 16 were observed to be asynchronous (20.78 %) and only 10 were found to be normal fertilized (12. 98 %).
  • ThesisItemOpen Access
    Studies on Freezability, Certain characteristics and fertility of buck spermatozoa
    (Birsa Agricultural University, 1986) Sinha, S. N.; Singh, B. K.
    A total of 11 bucks comprising 4 Jamnapari, 4 Barbari and 3 Black Bengal bucks were used in this study for the assessment of free ability of their sperms. Tris egg yolk fructose citric acid glycerol dilutor was used for the freezing of semen samples. Samples were packaged in Landshut straws, frozen and were preserved in liquid nitrogen. Cellular and acrosomal morphology of the sperms were studied at different stages of freezing i.e. after dilution, after equilibration and after thawing. Altogether, 132 semen samples were frozen. Of these, 36 semen samples were collected from bucks of Black Bengal breed and 48 semen samples each were collected from bucks of Jamnapari and Barbari breed. The effect of different hours of equilibration was studied on the characters of sperms. After freezing post thaw characters with main emphasis on motility was studied and was compared statistically for different hours of equilibration and different breeds. Assay of activities of three important enzymes viz AST, ALT and LDH was made in unfrozen and frozen semen samples following different hours of equilibration. A total of 99 semen samples representing different stages viz neat, diluted and frozen were
  • ThesisItemOpen Access
    Anoestrum in crossbred cattle and its response to treatment with secrodyl
    (Birsa Agricultural University, 1982) Singh, Jaswant; Singh, B.K.
    On the basis of the results obtained in the present study, it can be safely concluded that the optimum dose of Secrodyl is, 2 ml intramuscularly on the first day followed by 1 ml intramuscularly daily for next 3 days for the treatment of true anoestrum in cattle.
  • ThesisItemOpen Access
    Studies on Certain factors influencing conception rate in cows
    (Birsa Agricultural University, 1982) Singh, Jaswant; Singh, B.K.
    On the basis of the results obtained in the present experiment it was concluded that conception rate in cows can be increased significantly if administration of 500 mg of ampicillin one hour before insemination or 50 I.U. of oxytocin intramuscularly, five minutes before insemination or udder massage for 2 minutes, five minutes before insemination is practiced. Similarly, in the case of heifers conception rate can be remarkably increased if 50 1.U. of oxytocin is given intramascularly, five minutes before insemination. Great improvement in conception rate can be brought about both in cows and heifers if clitoral message is done for 10 seconds, five minutes before insemination