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  • ThesisItemOpen Access
    STUDIES ON PRODUCTION AND ANTIMICROBIAL PROPERTIES OF Volvariella volvacea (Bull.Ex.Fr.) Sing
    (COLLEGE OF HORTICULTURE AND FORESTRY, DR Y S P UHF, NERI, HAMIRPUR, 2019-10-23) SHARMA, SHIVANI; JARIAL, R S
    The present investigations entitled “Studies on production and antimicrobial properties of Volvariella volvacea (Bull.Ex.Fr.) Sing’’ was undertaken to evaluate various cultural requirements for the vegetative growth of different strains of V. volvacea, to evaluate different locally available substrates for the production of different strains of V. volvacea and to study antimicrobial properties of different strains of V. volvacea against five human pathogenic bacteria viz., E. Coli, Proteus sp., Bacillus sp., Pseudomonas sp. and Streptococcus sp. Among five liquid media studied, malt extract was found to be the best medium for the growth of all the strains of V. volvacea both in solid (38.92 mm) as well as liquid (2625.42 mg) phase. Out of five different pH levels evaluated for the growth of different strains of V. volvacea, pH 7.0 (30.75 mm) was observed to be the best pH for the growth of all the strains of the test fungus. Out of five different temperature regimes evaluated, 30°C (41.78 mm) was observed to be the ideal temperature for the growth of V. volvacea. Out of four strains under study DMR-484 was observed to grow best in all the nutrient media (Solid; 41.38 mm and Liquid; 1780.67 mg), different pH levels (29.64 mm) and different temperature regimes (37.00 mm) studied. In production trials, among five different substrates, cotton waste was observed to be the best substrate in terms of spawn run period (8.25 days), time taken for first flush (13.91 days), number of fruit bodies (35.50/bed), yield (472.91 g/Kg) and biological efficiency (15.76%). The aqueous and methanolic extracts of the mycelium of strain DMR-484 were found to have antimicrobial activities against E. coli with average zone of inhibition 10.33 mm and 13.00 mm, respectively. However, mycelia of all other strains were unable to inhibit any of five human pathogenic bacteria under study. Further, it was also found that none of the four fruit body extracts of all the strains under study exhibited antimicrobial activity against any of the human pathogenic bacteria.
  • ThesisItemOpen Access
    STUDIES ON BIOECONOMIC APPRAISAL AND EFFECT OF ORGANIC MANURE ON BIOMASS PRODUCTION OF Ocimum sanctum UNDER STONE FRUIT BASED AGROFORESTRY SYSTEM
    (UHF,NAUNI, 2017-07) SHARMA, SHIVANI; PANT, K.S.
    ABSTRACT The current study entitled “Studies on bioeconomic appraisal and effect of organic manure on biomass production of Ocimum sanctum under stone fruit based agroforestry system” was carried out at experimental farm of Department of Silviculture and Agroforestry, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan- 173230 (HP), India. The experiment was laid out in RBD with three replications. The study aim at exploring the possibilities of successful cultivation of medicinal plant as intercrop under two different systems along with usage of different doses of organic manure. The experiment comprised of 7 treatments, which included three different doses of FYM (15t/ha, 20t/ha and 25t/ha), three different doses of Jeevamrut (180ml/plant, 300ml/plant and 420ml/plant) and one control (no manre). The observations were taken at three distances from the tree i.e. 1m, 2m and 3m. The results revealed that MAPs can be grown successfully under agroforestry system. All the growth and yield parameter of medicinal plant were found highest in 420ml/plant Jeevamrut at a distance of 3m away from trees. The use of organic manures further improves the production ability of intercrop by minimizing competition for critical resources. Soil physical and chemical properties were found to be better in intercropping as compared to control. The availability of nutrients like N, P, K and Organic carbon were high where, higher doses of organic manures were applied in agroforestry system at a distance of one meter away from trees. Soil pH and organic carbon were also improved in agroforestry system than sole crop. The findings of present study suggested that cultivation of M&APs in combination with fruit trees, especially peach may be recommended for better economic return from agroforestry system. It also suggested that the agroforestry system have higher potential of producing biomass. Thus integrating M&APs with stone fruits offers an excellent opportunity for diversification and high income generation than monoculture. It will also open new vistas for the research to evaluate the performance of M&APs under tree canopy.